Cronobacter phage CR3
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 265 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I1TRU3|I1TRU3_9CAUD Uncharacterized protein OS=Cronobacter phage CR3 OX=1162295 GN=CR3_251 PE=4 SV=1
MM1 pKa = 7.95 LIMVDD6 pKa = 4.51 EE7 pKa = 4.61 EE8 pKa = 4.42 PEE10 pKa = 3.87 YY11 pKa = 10.84 DD12 pKa = 3.86 SPHH15 pKa = 5.92 GSVWIVAVDD24 pKa = 3.21 YY25 pKa = 10.87 AGRR28 pKa = 11.84 VTVLDD33 pKa = 4.51 PPNLHH38 pKa = 6.96 PGIMDD43 pKa = 4.49 NGPEE47 pKa = 3.92 AEE49 pKa = 4.33 YY50 pKa = 10.86 LGLPPDD56 pKa = 4.36 VDD58 pKa = 4.74 DD59 pKa = 4.97 MDD61 pKa = 4.96 PGVYY65 pKa = 9.04 RR66 pKa = 11.84 WTCSYY71 pKa = 8.49 HH72 pKa = 3.88 THH74 pKa = 6.75 IDD76 pKa = 3.65 RR77 pKa = 11.84 EE78 pKa = 4.4 SGHH81 pKa = 6.88 VDD83 pKa = 3.32 DD84 pKa = 5.82 YY85 pKa = 11.87 DD86 pKa = 4.28 FEE88 pKa = 5.34 IEE90 pKa = 4.2 EE91 pKa = 4.79 SVLLWSPDD99 pKa = 3.28 NAA101 pKa = 4.28
Molecular weight: 11.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.579
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.49
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.126
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.91
Patrickios 0.477
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|I1TRG9|I1TRG9_9CAUD Uncharacterized protein OS=Cronobacter phage CR3 OX=1162295 GN=CR3_127 PE=4 SV=1
MM1 pKa = 6.83 EE2 pKa = 6.31 HH3 pKa = 6.95 IYY5 pKa = 10.42 FYY7 pKa = 10.95 LLWVLFSVVGIWRR20 pKa = 11.84 MLSLMSVSGRR30 pKa = 11.84 VGNDD34 pKa = 2.92 DD35 pKa = 3.61 RR36 pKa = 11.84 VMLLFMLTPACFLMTPVLLFVEE58 pKa = 4.38 LMFWVGRR65 pKa = 11.84 KK66 pKa = 8.26 IDD68 pKa = 3.83 KK69 pKa = 10.2 FRR71 pKa = 11.84 EE72 pKa = 3.99 SPRR75 pKa = 11.84 YY76 pKa = 7.51 VTWKK80 pKa = 8.9 IQRR83 pKa = 11.84 QIRR86 pKa = 11.84 KK87 pKa = 8.73 RR88 pKa = 11.84 RR89 pKa = 11.84 RR90 pKa = 11.84 GPLVRR95 pKa = 11.84 VVEE98 pKa = 4.14 HH99 pKa = 6.02 LHH101 pKa = 5.8 NKK103 pKa = 9.66 RR104 pKa = 11.84 KK105 pKa = 9.97 EE106 pKa = 3.83 KK107 pKa = 9.98 TRR109 pKa = 11.84 EE110 pKa = 3.83 EE111 pKa = 4.17 NVPEE115 pKa = 4.17 VV116 pKa = 3.45
Molecular weight: 14.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.648
IPC_protein 10.423
Toseland 10.716
ProMoST 10.394
Dawson 10.804
Bjellqvist 10.496
Wikipedia 11.008
Rodwell 11.023
Grimsley 10.847
Solomon 10.921
Lehninger 10.891
Nozaki 10.687
DTASelect 10.496
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.73
Patrickios 10.774
IPC_peptide 10.935
IPC2_peptide 9.385
IPC2.peptide.svr19 8.676
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
265
0
265
43819
57
1093
165.4
18.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.54 ± 0.187
1.196 ± 0.076
6.52 ± 0.149
6.881 ± 0.219
4.238 ± 0.12
8.038 ± 0.358
1.874 ± 0.1
5.543 ± 0.133
6.513 ± 0.187
7.793 ± 0.169
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.088 ± 0.107
4.322 ± 0.13
4.142 ± 0.137
3.619 ± 0.133
5.144 ± 0.161
5.317 ± 0.162
5.714 ± 0.251
7.095 ± 0.181
1.655 ± 0.077
3.768 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here