Potato yellow vein virus
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q70PI3|Q70PI3_9CLOS Uncharacterized protein OS=Potato yellow vein virus OX=103881 PE=4 SV=1
MM1 pKa = 7.42 NSISEE6 pKa = 4.89 LITEE10 pKa = 4.71 FGEE13 pKa = 4.43 DD14 pKa = 3.08 RR15 pKa = 11.84 VDD17 pKa = 4.31 EE18 pKa = 4.32 IFALHH23 pKa = 6.91 RR24 pKa = 11.84 SFGQSNPPLCDD35 pKa = 3.03 VLLNLISSKK44 pKa = 10.57 LFSHH48 pKa = 7.48 DD49 pKa = 3.8 DD50 pKa = 3.5 VFEE53 pKa = 3.94 EE54 pKa = 4.9 SIIEE58 pKa = 4.17 FGDD61 pKa = 3.53 LKK63 pKa = 11.3 SFLLVTKK70 pKa = 8.56 FIRR73 pKa = 11.84 GVYY76 pKa = 8.84 NSRR79 pKa = 11.84 YY80 pKa = 7.51 NLRR83 pKa = 11.84 YY84 pKa = 9.72
Molecular weight: 9.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.795
IPC2_protein 4.863
IPC_protein 4.698
Toseland 4.571
ProMoST 4.813
Dawson 4.647
Bjellqvist 4.8
Wikipedia 4.533
Rodwell 4.558
Grimsley 4.482
Solomon 4.647
Lehninger 4.596
Nozaki 4.774
DTASelect 4.914
Thurlkill 4.571
EMBOSS 4.546
Sillero 4.825
Patrickios 3.948
IPC_peptide 4.66
IPC2_peptide 4.813
IPC2.peptide.svr19 4.828
Protein with the highest isoelectric point:
>tr|Q70PI4|Q70PI4_9CLOS p7 OS=Potato yellow vein virus OX=103881 PE=4 SV=1
MM1 pKa = 7.54 VSIFRR6 pKa = 11.84 SPVVRR11 pKa = 11.84 DD12 pKa = 3.65 FLSMVFKK19 pKa = 10.81 TNDD22 pKa = 3.26 PSEE25 pKa = 4.78 KK26 pKa = 9.28 IGEE29 pKa = 4.08 INKK32 pKa = 9.48 YY33 pKa = 8.84 LVKK36 pKa = 10.63 NFHH39 pKa = 7.02 IEE41 pKa = 3.92 NQNKK45 pKa = 9.06 YY46 pKa = 7.97 RR47 pKa = 11.84 TNIKK51 pKa = 10.31 GRR53 pKa = 11.84 LTEE56 pKa = 3.89 LTSNYY61 pKa = 9.01 FVRR64 pKa = 11.84 NNEE67 pKa = 3.53 IFVNDD72 pKa = 3.79 GNFLEE77 pKa = 4.32 ILKK80 pKa = 10.61 LPVIFMYY87 pKa = 10.66 RR88 pKa = 11.84 IRR90 pKa = 11.84 PEE92 pKa = 3.81 LTKK95 pKa = 10.96 NSAYY99 pKa = 10.84 LPVNIFASYY108 pKa = 10.83 NYY110 pKa = 9.87 EE111 pKa = 3.83 FYY113 pKa = 11.01 KK114 pKa = 10.99 NSFSPHH120 pKa = 5.54 YY121 pKa = 10.81 DD122 pKa = 3.0 KK123 pKa = 11.42 DD124 pKa = 4.14 LNSFLSSNKK133 pKa = 10.07 EE134 pKa = 3.48 IGCLYY139 pKa = 9.47 TEE141 pKa = 4.73 RR142 pKa = 11.84 DD143 pKa = 3.52 IQEE146 pKa = 4.86 HH147 pKa = 5.9 YY148 pKa = 10.51 PSVHH152 pKa = 5.33 GVRR155 pKa = 11.84 LLTLYY160 pKa = 10.08 RR161 pKa = 11.84 VCNSLGRR168 pKa = 11.84 YY169 pKa = 8.81 VDD171 pKa = 5.25 LNEE174 pKa = 5.15 LDD176 pKa = 3.48 NGNIKK181 pKa = 10.42 GFSIDD186 pKa = 3.88 PDD188 pKa = 3.8 SKK190 pKa = 11.5 AKK192 pKa = 10.39 IIGEE196 pKa = 4.23 GLSSNPLFNSCVEE209 pKa = 3.91 IFRR212 pKa = 11.84 SYY214 pKa = 11.63 LEE216 pKa = 4.12 LSATKK221 pKa = 10.42 AGNAKK226 pKa = 9.93 VEE228 pKa = 4.19 ANKK231 pKa = 10.64 KK232 pKa = 10.03 IFTTYY237 pKa = 10.09 LNCMSLGEE245 pKa = 4.21 SYY247 pKa = 11.03 EE248 pKa = 4.3 RR249 pKa = 11.84 IRR251 pKa = 11.84 KK252 pKa = 8.68 NPLVIAKK259 pKa = 8.84 FIKK262 pKa = 10.38 LYY264 pKa = 11.12 EE265 pKa = 4.24 EE266 pKa = 4.13 FTKK269 pKa = 10.52 EE270 pKa = 3.86 SRR272 pKa = 11.84 GFKK275 pKa = 10.51 DD276 pKa = 3.2 DD277 pKa = 4.09 FEE279 pKa = 6.81 AIKK282 pKa = 10.87 LLTPRR287 pKa = 11.84 FKK289 pKa = 11.24 VFLKK293 pKa = 10.68 KK294 pKa = 10.9 VFDD297 pKa = 4.31 VNSSLNDD304 pKa = 4.36 DD305 pKa = 3.68 ILFIEE310 pKa = 4.63 MPKK313 pKa = 10.56 SSVVDD318 pKa = 4.03 LLSEE322 pKa = 3.98 PVSVGEE328 pKa = 3.99 YY329 pKa = 10.28 LRR331 pKa = 11.84 SKK333 pKa = 10.66 DD334 pKa = 3.7 AISVVSTSNTLSEE347 pKa = 4.43 KK348 pKa = 10.86 ADD350 pKa = 3.87 LLATHH355 pKa = 7.4 IILRR359 pKa = 11.84 FLQDD363 pKa = 3.82 KK364 pKa = 10.66 LPAPEE369 pKa = 6.08 DD370 pKa = 3.4 ILIDD374 pKa = 3.96 AFLMILGRR382 pKa = 11.84 TTTNSKK388 pKa = 10.61 RR389 pKa = 11.84 FGKK392 pKa = 9.66 PVLVTLKK399 pKa = 10.27 YY400 pKa = 10.76 EE401 pKa = 4.15 NMSKK405 pKa = 10.51 EE406 pKa = 3.47 IDD408 pKa = 3.13 ISVVVNALNNKK419 pKa = 9.07 VSGAHH424 pKa = 6.02 NEE426 pKa = 3.9 YY427 pKa = 10.9 LGMNVLRR434 pKa = 11.84 LWANSRR440 pKa = 11.84 SSRR443 pKa = 11.84 AMRR446 pKa = 11.84 LFRR449 pKa = 11.84 SLNFNPGLFSYY460 pKa = 10.92 CPGILDD466 pKa = 3.34 YY467 pKa = 11.03 MRR469 pKa = 11.84 FDD471 pKa = 4.51 FYY473 pKa = 11.53 KK474 pKa = 10.77 AIPLKK479 pKa = 11.08 DD480 pKa = 3.41 MTDD483 pKa = 3.48 EE484 pKa = 4.22 EE485 pKa = 4.69 VQSFRR490 pKa = 11.84 TLRR493 pKa = 11.84 LHH495 pKa = 5.23 TEE497 pKa = 3.73 KK498 pKa = 10.34 RR499 pKa = 11.84 TDD501 pKa = 3.42 PQGSYY506 pKa = 10.35 SAEE509 pKa = 3.79 CDD511 pKa = 3.01 NWILNLKK518 pKa = 9.13 NRR520 pKa = 11.84 SS521 pKa = 3.4
Molecular weight: 60.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.033
IPC2_protein 8.141
IPC_protein 8.024
Toseland 8.434
ProMoST 8.536
Dawson 8.887
Bjellqvist 8.873
Wikipedia 9.004
Rodwell 9.004
Grimsley 8.697
Solomon 8.975
Lehninger 8.946
Nozaki 8.946
DTASelect 8.741
Thurlkill 8.829
EMBOSS 9.048
Sillero 9.033
Patrickios 4.469
IPC_peptide 8.975
IPC2_peptide 7.541
IPC2.peptide.svr19 7.617
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
4944
35
2475
494.4
56.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.236 ± 0.32
1.82 ± 0.197
6.614 ± 0.333
5.643 ± 0.311
5.542 ± 0.348
4.632 ± 0.228
1.517 ± 0.089
7.201 ± 0.254
8.212 ± 0.287
9.79 ± 0.461
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.488 ± 0.311
6.472 ± 0.508
3.196 ± 0.249
2.447 ± 0.457
5.34 ± 0.386
9.244 ± 0.25
4.693 ± 0.37
7.019 ± 0.225
0.445 ± 0.089
4.45 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here