Human rotavirus G9P[8]

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A; Rotavirus G9

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E7D6Q2|E7D6Q2_9REOV Outer capsid glycoprotein VP7 (Fragment) OS=Human rotavirus G9P[8] OX=408599 PE=3 SV=1
MM1 pKa = 7.26YY2 pKa = 10.17GIEE5 pKa = 4.25YY6 pKa = 6.55TTVLIFLISIVLLNYY21 pKa = 9.52ILKK24 pKa = 10.22SLTSAMDD31 pKa = 3.66FIIYY35 pKa = 10.15RR36 pKa = 11.84FLLLVVIVSPFVKK49 pKa = 8.86TQNYY53 pKa = 9.32GINLPITGSMDD64 pKa = 3.06TAYY67 pKa = 10.6ANSSQQEE74 pKa = 4.24TFLTSTLCLYY84 pKa = 10.82YY85 pKa = 8.95PTEE88 pKa = 4.46ASTQIGDD95 pKa = 4.25TEE97 pKa = 4.35WKK99 pKa = 8.5DD100 pKa = 3.45TLSQLFLTKK109 pKa = 10.05GWPTGSVYY117 pKa = 10.43FKK119 pKa = 10.07EE120 pKa = 4.35YY121 pKa = 11.07ADD123 pKa = 3.5IASFSIDD130 pKa = 3.27PQLYY134 pKa = 8.88CDD136 pKa = 3.92YY137 pKa = 11.0NVVLMKK143 pKa = 10.51YY144 pKa = 10.55DD145 pKa = 3.53STLEE149 pKa = 3.89LDD151 pKa = 3.63MSEE154 pKa = 4.63LADD157 pKa = 5.13LILNEE162 pKa = 4.24WLCNPMDD169 pKa = 3.29ITLYY173 pKa = 10.67YY174 pKa = 9.25YY175 pKa = 10.55QQTDD179 pKa = 3.57EE180 pKa = 4.17ANKK183 pKa = 8.45WISMGQSCTIKK194 pKa = 10.31VCPLNTQTLGIGCTTTNTATFEE216 pKa = 3.92EE217 pKa = 5.14VAASEE222 pKa = 4.37KK223 pKa = 10.87LVITDD228 pKa = 3.23VVDD231 pKa = 4.31GVNHH235 pKa = 6.68KK236 pKa = 10.91LDD238 pKa = 3.67VTTNTCTIRR247 pKa = 11.84NCRR250 pKa = 11.84KK251 pKa = 9.67LGPRR255 pKa = 11.84EE256 pKa = 3.78NVAIIQVGGSEE267 pKa = 4.29VLDD270 pKa = 3.39ITADD274 pKa = 3.59PTTAPQTEE282 pKa = 3.66RR283 pKa = 11.84MMRR286 pKa = 11.84INWKK290 pKa = 9.54KK291 pKa = 7.26WWQVFYY297 pKa = 10.65TVVDD301 pKa = 3.96YY302 pKa = 11.12INQIVQVMSKK312 pKa = 10.17RR313 pKa = 11.84SRR315 pKa = 11.84SLNSAAFYY323 pKa = 11.0YY324 pKa = 10.27RR325 pKa = 11.84VV326 pKa = 3.19

Molecular weight: 37.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E7D6K4|E7D6K4_9REOV Non-structural protein 3 OS=Human rotavirus G9P[8] OX=408599 PE=3 SV=1
MM1 pKa = 7.81AEE3 pKa = 4.64LACFCYY9 pKa = 9.75PHH11 pKa = 7.6LEE13 pKa = 3.86NDD15 pKa = 3.51SYY17 pKa = 11.97KK18 pKa = 10.64FIPFNSLAIKK28 pKa = 10.62CMLTAKK34 pKa = 9.91VDD36 pKa = 3.91KK37 pKa = 10.63KK38 pKa = 11.12DD39 pKa = 3.04QDD41 pKa = 3.13KK42 pKa = 10.47FYY44 pKa = 11.27NSIVYY49 pKa = 9.72GIAPPPQFKK58 pKa = 10.26KK59 pKa = 10.53RR60 pKa = 11.84YY61 pKa = 5.69NTNDD65 pKa = 2.8NSRR68 pKa = 11.84GMNYY72 pKa = 6.95EE73 pKa = 3.62TPMFNKK79 pKa = 9.66VAILICEE86 pKa = 4.24ALNSIKK92 pKa = 9.45VTQSDD97 pKa = 4.06VANVLSRR104 pKa = 11.84VVSVRR109 pKa = 11.84HH110 pKa = 5.93LEE112 pKa = 3.89NLVLRR117 pKa = 11.84KK118 pKa = 9.59EE119 pKa = 4.02NHH121 pKa = 5.63QDD123 pKa = 3.26VLFHH127 pKa = 7.13SKK129 pKa = 10.37EE130 pKa = 4.08LLLKK134 pKa = 10.57AVLIAIGQSKK144 pKa = 10.17EE145 pKa = 3.74IEE147 pKa = 4.2TTATAEE153 pKa = 4.08GGEE156 pKa = 4.08ITFQNAAFTMWKK168 pKa = 8.49LTYY171 pKa = 9.82LDD173 pKa = 4.31HH174 pKa = 8.04KK175 pKa = 10.88LMPILDD181 pKa = 3.78QNFIEE186 pKa = 5.0YY187 pKa = 10.32KK188 pKa = 8.98ITLNEE193 pKa = 4.63DD194 pKa = 3.2KK195 pKa = 10.04PLSDD199 pKa = 3.09ICVKK203 pKa = 10.58EE204 pKa = 4.01LVAEE208 pKa = 4.68LRR210 pKa = 11.84WQYY213 pKa = 11.68NRR215 pKa = 11.84FAVITHH221 pKa = 6.3GKK223 pKa = 7.43GHH225 pKa = 5.73YY226 pKa = 9.48RR227 pKa = 11.84VVKK230 pKa = 8.49YY231 pKa = 10.96SSVANHH237 pKa = 7.18ADD239 pKa = 3.4RR240 pKa = 11.84VFATYY245 pKa = 10.71KK246 pKa = 10.95NNVKK250 pKa = 10.32SGNVTDD256 pKa = 6.56FNLLDD261 pKa = 5.15QRR263 pKa = 11.84IIWQNWYY270 pKa = 10.72AFTSSMKK277 pKa = 10.28QGNTLDD283 pKa = 3.59VCKK286 pKa = 10.66KK287 pKa = 10.71LLFQKK292 pKa = 9.76MKK294 pKa = 10.47QEE296 pKa = 4.09KK297 pKa = 10.31NPFKK301 pKa = 10.91GLSTDD306 pKa = 2.99RR307 pKa = 11.84KK308 pKa = 8.05MDD310 pKa = 3.64EE311 pKa = 4.1VSHH314 pKa = 6.83VGII317 pKa = 4.83

Molecular weight: 36.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

3728

174

568

338.9

39.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.614 ± 0.585

1.583 ± 0.41

6.357 ± 0.426

5.231 ± 0.517

4.426 ± 0.39

3.353 ± 0.378

1.77 ± 0.307

7.806 ± 0.315

6.733 ± 0.885

9.067 ± 0.447

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.843 ± 0.328

7.323 ± 0.522

3.273 ± 0.364

4.024 ± 0.352

4.265 ± 0.427

7.645 ± 0.88

6.786 ± 0.633

6.411 ± 0.295

1.395 ± 0.163

5.097 ± 0.483
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski