Athtab bunya-like virus
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G1WQW8|A0A3G1WQW8_9VIRU Glycoprotein C OS=Athtab bunya-like virus OX=2315724 PE=4 SV=1
MM1 pKa = 7.58 LKK3 pKa = 9.25 TVFLLLTSTTLLVDD17 pKa = 3.41 NAVVMTSMGGRR28 pKa = 11.84 YY29 pKa = 9.01 RR30 pKa = 11.84 LCFGSFCYY38 pKa = 10.58 YY39 pKa = 10.71 SEE41 pKa = 3.83 FAAYY45 pKa = 9.44 KK46 pKa = 9.97 EE47 pKa = 4.05 QFEE50 pKa = 4.23 AQAAFQCAHH59 pKa = 7.1 LLANIVAIDD68 pKa = 4.01 TTTSTCSVYY77 pKa = 10.58 KK78 pKa = 10.46 PRR80 pKa = 11.84 ACFAQVEE87 pKa = 4.52 QGEE90 pKa = 4.74 GPYY93 pKa = 10.29 LVPFDD98 pKa = 3.91 YY99 pKa = 10.93 TILQSTNFIPAHH111 pKa = 6.39 LGCITYY117 pKa = 9.42 TSSQNTFAYY126 pKa = 6.92 KK127 pKa = 9.6 TCSTPSALKK136 pKa = 10.45 DD137 pKa = 3.47 PSVVVPITTTTTTFSFTVYY156 pKa = 10.71 KK157 pKa = 10.52 DD158 pKa = 3.74 YY159 pKa = 10.88 TCEE162 pKa = 3.94 AVLSPTWTTTVWTDD176 pKa = 2.9 TKK178 pKa = 10.84 IVITSTIVTNLITSITVVNCTITNTHH204 pKa = 6.86 LSIQGYY210 pKa = 6.75 TLTTTEE216 pKa = 4.69 LLLTTALAKK225 pKa = 10.19 PAVTNITSVTFTTTQLNIVNHH246 pKa = 5.93 VLTTTSVLFTEE257 pKa = 4.56 TTYY260 pKa = 10.89 TITEE264 pKa = 3.94 YY265 pKa = 10.81 HH266 pKa = 6.14 HH267 pKa = 6.61 TVTHH271 pKa = 5.94 ADD273 pKa = 3.5 EE274 pKa = 4.77 YY275 pKa = 10.44 KK276 pKa = 10.15 TLEE279 pKa = 4.15 PVLDD283 pKa = 3.82 RR284 pKa = 11.84 KK285 pKa = 8.23 TCNLLGMHH293 pKa = 6.87 FCDD296 pKa = 5.24 KK297 pKa = 10.57 CLPPNKK303 pKa = 9.13 ICNGIKK309 pKa = 10.32 DD310 pKa = 4.22 CADD313 pKa = 3.47 GTDD316 pKa = 3.83 EE317 pKa = 5.48 PSNCC321 pKa = 4.38
Molecular weight: 35.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.431
IPC2_protein 5.347
IPC_protein 5.321
Toseland 5.537
ProMoST 5.626
Dawson 5.512
Bjellqvist 5.563
Wikipedia 5.486
Rodwell 5.474
Grimsley 5.588
Solomon 5.512
Lehninger 5.499
Nozaki 5.741
DTASelect 5.919
Thurlkill 5.842
EMBOSS 5.804
Sillero 5.83
Patrickios 0.337
IPC_peptide 5.525
IPC2_peptide 5.842
IPC2.peptide.svr19 5.738
Protein with the highest isoelectric point:
>tr|A0A3G1WQW8|A0A3G1WQW8_9VIRU Glycoprotein C OS=Athtab bunya-like virus OX=2315724 PE=4 SV=1
MM1 pKa = 7.72 RR2 pKa = 11.84 NILEE6 pKa = 4.24 KK7 pKa = 10.87 SSMYY11 pKa = 10.17 MLILLFVEE19 pKa = 5.02 LVVSSGVFVKK29 pKa = 10.05 TNNCTSLEE37 pKa = 4.03 KK38 pKa = 10.59 PQDD41 pKa = 3.6 CEE43 pKa = 3.99 EE44 pKa = 4.11 FGEE47 pKa = 4.35 LGGVHH52 pKa = 6.06 WARR55 pKa = 11.84 LKK57 pKa = 10.86 DD58 pKa = 3.64 RR59 pKa = 11.84 QDD61 pKa = 3.06 KK62 pKa = 11.45 YY63 pKa = 11.13 EE64 pKa = 4.05 IQAKK68 pKa = 10.38 LSGPVCTGFSDD79 pKa = 5.29 PILIGDD85 pKa = 3.7 SRR87 pKa = 11.84 FSYY90 pKa = 10.82 SVDD93 pKa = 3.03 GQFRR97 pKa = 11.84 SLSGEE102 pKa = 4.04 VVDD105 pKa = 5.43 RR106 pKa = 11.84 CSQVEE111 pKa = 3.83 NDD113 pKa = 4.52 HH114 pKa = 6.9 FPEE117 pKa = 4.98 CGTAVVSVCHH127 pKa = 7.08 LIPIGNCEE135 pKa = 4.2 LKK137 pKa = 10.73 VDD139 pKa = 4.39 LEE141 pKa = 4.32 SSKK144 pKa = 10.26 VHH146 pKa = 5.84 VSGKK150 pKa = 8.94 TSGPLQYY157 pKa = 10.51 VIQINEE163 pKa = 3.99 EE164 pKa = 3.59 VDD166 pKa = 3.73 MEE168 pKa = 4.35 GHH170 pKa = 6.69 CLNCEE175 pKa = 4.1 GFSRR179 pKa = 11.84 SYY181 pKa = 10.55 PLQEE185 pKa = 4.42 GLTTYY190 pKa = 10.94 HH191 pKa = 6.28 LTCNRR196 pKa = 11.84 NSLSKK201 pKa = 9.58 TLSLSKK207 pKa = 10.91 SEE209 pKa = 4.0 VCARR213 pKa = 11.84 NAQDD217 pKa = 4.13 FYY219 pKa = 11.73 LGFHH223 pKa = 6.41 YY224 pKa = 9.54 KK225 pKa = 8.93 WCMNAFWYY233 pKa = 7.7 KK234 pKa = 9.74 TLTILFFLVMLTACVPMINWPIHH257 pKa = 5.46 MILNKK262 pKa = 10.4 LIRR265 pKa = 11.84 CCSRR269 pKa = 11.84 SEE271 pKa = 4.08 AKK273 pKa = 10.74 VKK275 pKa = 8.59 VTKK278 pKa = 10.36 AVPSFLFLIWWVVLLSHH295 pKa = 6.93 IVYY298 pKa = 10.71 AEE300 pKa = 3.92 ASLADD305 pKa = 3.63 SLKK308 pKa = 9.92 EE309 pKa = 3.77 QGFKK313 pKa = 10.97 PEE315 pKa = 4.01 TVDD318 pKa = 4.49 FKK320 pKa = 11.37 GLYY323 pKa = 7.78 RR324 pKa = 11.84 TTVPEE329 pKa = 4.24 ADD331 pKa = 3.99 FEE333 pKa = 5.22 LISTGTGEE341 pKa = 4.23 ALRR344 pKa = 11.84 VEE346 pKa = 4.36 SFDD349 pKa = 3.64 IKK351 pKa = 10.84 LSPGYY356 pKa = 10.79 ALGLVVDD363 pKa = 4.4 NVEE366 pKa = 4.56 LDD368 pKa = 3.96 LKK370 pKa = 10.18 VKK372 pKa = 9.63 SVRR375 pKa = 11.84 AQMSYY380 pKa = 10.74 KK381 pKa = 10.34 DD382 pKa = 4.23 CYY384 pKa = 9.81 STGDD388 pKa = 3.26 TKK390 pKa = 10.69 IISKK394 pKa = 8.5 STDD397 pKa = 2.9 TCTEE401 pKa = 3.84 SCDD404 pKa = 3.44 KK405 pKa = 10.85 CVEE408 pKa = 4.09 EE409 pKa = 4.87 LVVPEE414 pKa = 4.05 QVLPEE419 pKa = 4.12 NVVKK423 pKa = 10.56 VAKK426 pKa = 10.02 SSSWACDD433 pKa = 3.21 GAGCLSMATGCTCGYY448 pKa = 10.73 CYY450 pKa = 7.56 MTLTGPVWKK459 pKa = 10.78 VCDD462 pKa = 3.88 LEE464 pKa = 4.3 LEE466 pKa = 4.15 KK467 pKa = 11.32 VEE469 pKa = 5.64 INLCFNIGGKK479 pKa = 9.32 GMCKK483 pKa = 9.06 WLEE486 pKa = 4.15 SVEE489 pKa = 4.23 TFADD493 pKa = 3.58 GLYY496 pKa = 10.36 QINLKK501 pKa = 5.61 TTKK504 pKa = 9.56 IVLPEE509 pKa = 3.85 RR510 pKa = 11.84 VAEE513 pKa = 4.12 VMEE516 pKa = 4.38 EE517 pKa = 3.98 GKK519 pKa = 10.51 KK520 pKa = 9.42 IRR522 pKa = 11.84 KK523 pKa = 8.2 IGLMNKK529 pKa = 10.02 LGEE532 pKa = 4.22 FDD534 pKa = 4.82 SEE536 pKa = 4.41 FGDD539 pKa = 3.9 FQVVDD544 pKa = 3.61 AGSNYY549 pKa = 10.12 EE550 pKa = 3.99 KK551 pKa = 10.9 DD552 pKa = 3.05 LDD554 pKa = 4.45 YY555 pKa = 11.23 IHH557 pKa = 7.07 EE558 pKa = 4.37 CHH560 pKa = 5.76 FTQHH564 pKa = 5.8 RR565 pKa = 11.84 RR566 pKa = 11.84 ITFKK570 pKa = 10.99 SCGQNHH576 pKa = 6.5 FYY578 pKa = 10.41 KK579 pKa = 10.56 HH580 pKa = 6.36 KK581 pKa = 10.37 NLHH584 pKa = 5.9 PCQTCFDD591 pKa = 4.22 GAKK594 pKa = 10.75 GMDD597 pKa = 3.85 FVDD600 pKa = 4.44 RR601 pKa = 11.84 PIGEE605 pKa = 4.37 AQLKK609 pKa = 10.61 LNLPLLGLSLKK620 pKa = 9.76 EE621 pKa = 4.29 DD622 pKa = 3.43 KK623 pKa = 11.32 VDD625 pKa = 3.4 VSKK628 pKa = 11.12 FKK630 pKa = 10.7 ISSCKK635 pKa = 9.99 GCRR638 pKa = 11.84 NCQEE642 pKa = 3.87 GMACKK647 pKa = 10.21 ISFEE651 pKa = 4.44 TKK653 pKa = 9.3 QSGRR657 pKa = 11.84 LPIKK661 pKa = 10.3 CDD663 pKa = 3.12 SAQSRR668 pKa = 11.84 NYY670 pKa = 10.62 LLAKK674 pKa = 9.79 IGKK677 pKa = 6.57 NTLMMTFFTDD687 pKa = 3.69 LKK689 pKa = 10.81 EE690 pKa = 4.34 GVLKK694 pKa = 11.14 CEE696 pKa = 4.21 LGMLKK701 pKa = 10.07 SQVNFKK707 pKa = 9.75 TEE709 pKa = 3.99 PGKK712 pKa = 10.86 LLLEE716 pKa = 4.42 DD717 pKa = 3.93 FRR719 pKa = 11.84 RR720 pKa = 11.84 AVDD723 pKa = 3.47 RR724 pKa = 11.84 SLEE727 pKa = 4.02 EE728 pKa = 5.71 DD729 pKa = 3.78 YY730 pKa = 11.13 HH731 pKa = 7.11 CQLFSCTMGRR741 pKa = 11.84 FLYY744 pKa = 10.77 NVFGLKK750 pKa = 10.42 GLWHH754 pKa = 5.98 YY755 pKa = 10.97 LWIIFIFVILLVVVVKK771 pKa = 10.19 LVKK774 pKa = 10.63 LILNRR779 pKa = 11.84 ATKK782 pKa = 10.49 RR783 pKa = 11.84 SMPIFKK789 pKa = 10.12 DD790 pKa = 3.16 SRR792 pKa = 11.84 FFKK795 pKa = 9.72 PQSVNVRR802 pKa = 3.49
Molecular weight: 90.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.417
IPC2_protein 6.605
IPC_protein 6.517
Toseland 6.376
ProMoST 7.102
Dawson 7.088
Bjellqvist 7.351
Wikipedia 7.029
Rodwell 7.102
Grimsley 6.427
Solomon 7.102
Lehninger 7.117
Nozaki 7.702
DTASelect 7.366
Thurlkill 7.41
EMBOSS 7.395
Sillero 7.629
Patrickios 3.935
IPC_peptide 7.102
IPC2_peptide 7.015
IPC2.peptide.svr19 6.86
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3358
321
2235
1119.3
128.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.318 ± 0.322
2.74 ± 1.053
5.956 ± 0.669
6.82 ± 0.55
5.092 ± 0.102
4.795 ± 0.658
2.144 ± 0.059
6.015 ± 0.5
7.683 ± 0.595
10.125 ± 0.271
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.008 ± 0.411
5.152 ± 0.643
3.008 ± 0.186
3.127 ± 0.095
4.586 ± 0.811
7.385 ± 0.194
5.926 ± 2.335
6.968 ± 0.636
1.37 ± 0.135
3.782 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here