Pseudobacter ginsenosidimutans

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Pseudobacter

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6020 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7N1C8|A0A4Q7N1C8_9BACT Mannose-6-phosphate isomerase class I OS=Pseudobacter ginsenosidimutans OX=661488 GN=EV199_1275 PE=4 SV=1
MM1 pKa = 7.48AFDD4 pKa = 4.54GPTRR8 pKa = 11.84PYY10 pKa = 10.7QEE12 pKa = 5.94DD13 pKa = 3.61DD14 pKa = 3.56TDD16 pKa = 3.62VLTSVEE22 pKa = 4.73EE23 pKa = 4.16PCSLIVWNDD32 pKa = 3.29EE33 pKa = 4.05VNTFEE38 pKa = 4.46WVIEE42 pKa = 3.97TLMAVCGHH50 pKa = 5.82SAEE53 pKa = 4.28QAEE56 pKa = 4.12QCAYY60 pKa = 9.82IIHH63 pKa = 6.14FQGKK67 pKa = 8.37YY68 pKa = 9.19AVKK71 pKa = 10.58NGTFDD76 pKa = 3.59EE77 pKa = 5.24LKK79 pKa = 9.33PQCDD83 pKa = 4.15AITDD87 pKa = 3.78RR88 pKa = 11.84GIGATIEE95 pKa = 4.17VVAAA99 pKa = 4.21

Molecular weight: 10.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7MY70|A0A4Q7MY70_9BACT Peptidase M28-like protein OS=Pseudobacter ginsenosidimutans OX=661488 GN=EV199_3995 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 9.56RR3 pKa = 11.84TFQPHH8 pKa = 4.58RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 6.91TVHH16 pKa = 6.14GFRR19 pKa = 11.84KK20 pKa = 10.11RR21 pKa = 11.84MQTANGRR28 pKa = 11.84KK29 pKa = 8.71VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.04GRR39 pKa = 11.84KK40 pKa = 8.77KK41 pKa = 10.08LTVSDD46 pKa = 3.83EE47 pKa = 4.55RR48 pKa = 11.84KK49 pKa = 10.22LKK51 pKa = 10.73

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6020

0

6020

2375140

29

3773

394.5

44.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.723 ± 0.032

0.738 ± 0.009

5.145 ± 0.02

5.476 ± 0.028

4.826 ± 0.021

7.059 ± 0.034

1.772 ± 0.016

6.679 ± 0.024

6.277 ± 0.03

9.455 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.443 ± 0.014

5.648 ± 0.032

4.104 ± 0.019

4.002 ± 0.019

4.563 ± 0.017

6.497 ± 0.024

5.906 ± 0.03

6.291 ± 0.024

1.346 ± 0.011

4.05 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski