Streptococcus phage Javan221

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A3E0|A0A4D6A3E0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan221 OX=2548053 GN=Javan221_0027 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 5.82KK3 pKa = 10.09SQKK6 pKa = 7.37TWEE9 pKa = 4.06IDD11 pKa = 3.17GEE13 pKa = 4.25LWLNCPVCGAEE24 pKa = 3.91VRR26 pKa = 11.84DD27 pKa = 3.55YY28 pKa = 10.9DD29 pKa = 3.95ICDD32 pKa = 3.14RR33 pKa = 11.84CGWQNTGEE41 pKa = 4.19TNIDD45 pKa = 3.37GGPNKK50 pKa = 8.17MTLAEE55 pKa = 4.27AKK57 pKa = 9.34EE58 pKa = 4.24AYY60 pKa = 10.11AKK62 pKa = 10.7GLEE65 pKa = 4.34IYY67 pKa = 10.5

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A505|A0A4D6A505_9CAUD Putative repressor protein OS=Streptococcus phage Javan221 OX=2548053 GN=Javan221_0048 PE=4 SV=1
MM1 pKa = 7.65ISLADD6 pKa = 3.76EE7 pKa = 5.23LEE9 pKa = 4.16QQVKK13 pKa = 8.78GWRR16 pKa = 11.84ARR18 pKa = 11.84YY19 pKa = 8.87FGLSHH24 pKa = 6.82EE25 pKa = 4.64FGEE28 pKa = 5.7IINQQQDD35 pKa = 3.77KK36 pKa = 10.59IISLQQEE43 pKa = 4.12NKK45 pKa = 9.49RR46 pKa = 11.84LKK48 pKa = 10.61RR49 pKa = 11.84EE50 pKa = 3.18IWNLKK55 pKa = 8.6KK56 pKa = 10.64IKK58 pKa = 10.15GKK60 pKa = 10.35RR61 pKa = 11.84RR62 pKa = 3.29

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10637

46

1419

204.6

22.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.944 ± 0.564

0.517 ± 0.092

5.932 ± 0.281

7.418 ± 0.692

3.742 ± 0.284

6.759 ± 0.461

1.222 ± 0.136

6.261 ± 0.297

7.69 ± 0.527

8.076 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.04 ± 0.194

6.083 ± 0.34

2.773 ± 0.179

4.419 ± 0.238

3.648 ± 0.342

7.164 ± 0.544

6.571 ± 0.561

6.675 ± 0.252

1.241 ± 0.093

3.826 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski