Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13500 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B8N153|B8N153_ASPFN Chitinase OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=AFLA_028280 PE=3 SV=1
MM1 pKa = 7.7 ADD3 pKa = 3.51 EE4 pKa = 5.26 AGSIYY9 pKa = 10.71 DD10 pKa = 3.85 EE11 pKa = 4.95 IEE13 pKa = 4.34 IEE15 pKa = 5.79 DD16 pKa = 3.79 MTFDD20 pKa = 6.24 PITQLYY26 pKa = 9.73 HH27 pKa = 5.63 YY28 pKa = 7.55 PCPCGDD34 pKa = 3.67 RR35 pKa = 11.84 FEE37 pKa = 5.7 IMIDD41 pKa = 3.49 DD42 pKa = 4.19 LRR44 pKa = 11.84 DD45 pKa = 3.58 GEE47 pKa = 4.73 EE48 pKa = 3.84 IAVCPSCSLRR58 pKa = 11.84 IRR60 pKa = 11.84 VIFDD64 pKa = 4.07 LDD66 pKa = 4.0 DD67 pKa = 3.49 LHH69 pKa = 9.1 KK70 pKa = 10.81 DD71 pKa = 3.74 DD72 pKa = 4.55 QQQGPSAVAVQAA84 pKa = 4.09
Molecular weight: 9.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.897
Patrickios 0.896
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|B8NXI9|B8NXI9_ASPFN Uncharacterized protein OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=AFLA_008080 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 10.37 LRR4 pKa = 11.84 WRR6 pKa = 11.84 WNRR9 pKa = 11.84 RR10 pKa = 11.84 VMHH13 pKa = 6.18 SFRR16 pKa = 11.84 AGFRR20 pKa = 11.84 LLINFPIHH28 pKa = 5.62 TLPPHH33 pKa = 6.21 GAFHH37 pKa = 7.41 LISAALVLSSLPILRR52 pKa = 11.84 KK53 pKa = 9.8 LL54 pKa = 3.5
Molecular weight: 6.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.468
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.398
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.149
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.12
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13500
0
13500
5679895
48
7763
420.7
46.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.266 ± 0.016
1.323 ± 0.008
5.585 ± 0.016
6.078 ± 0.019
3.826 ± 0.014
6.874 ± 0.019
2.42 ± 0.009
5.128 ± 0.015
4.627 ± 0.017
9.172 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.205 ± 0.008
3.724 ± 0.012
5.839 ± 0.022
3.995 ± 0.015
5.978 ± 0.02
8.199 ± 0.024
5.956 ± 0.013
6.313 ± 0.016
1.537 ± 0.008
2.954 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here