Ilyobacter polytropus (strain ATCC 51220 / DSM 2926 / LMG 16218 / CuHBu1)
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2859 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E3H6Z4|E3H6Z4_ILYPC Binding-protein-dependent transport systems inner membrane component OS=Ilyobacter polytropus (strain ATCC 51220 / DSM 2926 / LMG 16218 / CuHBu1) OX=572544 GN=Ilyop_0726 PE=3 SV=1
MM1 pKa = 7.0 KK2 pKa = 10.14 RR3 pKa = 11.84 VMIFLMFLILVSGMAYY19 pKa = 10.08 SEE21 pKa = 4.06 TRR23 pKa = 11.84 TVYY26 pKa = 10.95 LIVPADD32 pKa = 3.91 AGTLSYY38 pKa = 11.05 YY39 pKa = 10.98 AVDD42 pKa = 3.76 EE43 pKa = 4.62 YY44 pKa = 11.01 EE45 pKa = 5.0 AEE47 pKa = 4.29 VTITASTEE55 pKa = 3.91 TTSYY59 pKa = 10.91 LGNSNLKK66 pKa = 10.4 LVTLDD71 pKa = 4.03 ASTSDD76 pKa = 3.49 TFKK79 pKa = 11.42 LMAKK83 pKa = 10.15 YY84 pKa = 10.21 KK85 pKa = 10.98 LNDD88 pKa = 3.03 KK89 pKa = 10.85 YY90 pKa = 11.73 YY91 pKa = 10.94 FLISSNTDD99 pKa = 3.0 TEE101 pKa = 4.45 SDD103 pKa = 3.77 DD104 pKa = 3.6 NLEE107 pKa = 3.86 ILEE110 pKa = 4.46 TNFVSTNSPSEE121 pKa = 4.11 VYY123 pKa = 10.67 NVFGSFDD130 pKa = 4.45 DD131 pKa = 3.99 YY132 pKa = 11.52 PGIKK136 pKa = 9.61 IQDD139 pKa = 3.82 DD140 pKa = 3.81 VRR142 pKa = 11.84 LSMIPVTDD150 pKa = 3.69 EE151 pKa = 3.72 FTEE154 pKa = 4.54 FTDD157 pKa = 5.24 GIVDD161 pKa = 3.8 EE162 pKa = 5.58 VYY164 pKa = 10.92 LSDD167 pKa = 6.13 DD168 pKa = 3.75 DD169 pKa = 3.73 EE170 pKa = 4.9 TYY172 pKa = 11.77 NNVAVNGYY180 pKa = 10.8 AGISPNNSTTFTIKK194 pKa = 10.62 EE195 pKa = 4.12 KK196 pKa = 10.75 DD197 pKa = 3.6 SEE199 pKa = 4.6 TEE201 pKa = 3.93 TVYY204 pKa = 11.13 TDD206 pKa = 4.14 LKK208 pKa = 10.0 TISDD212 pKa = 4.58 PLTYY216 pKa = 10.36 SYY218 pKa = 11.43 TDD220 pKa = 3.72 DD221 pKa = 5.48 DD222 pKa = 5.51 GNDD225 pKa = 3.43 HH226 pKa = 6.08 YY227 pKa = 10.12 TVTVTYY233 pKa = 10.58 AQFRR237 pKa = 11.84 AKK239 pKa = 8.9 NTNSSTFTYY248 pKa = 10.33 DD249 pKa = 3.0 GKK251 pKa = 10.72 NDD253 pKa = 3.52 GNEE256 pKa = 4.28 TYY258 pKa = 10.12 TLTGEE263 pKa = 4.32 MVLDD267 pKa = 4.12 LYY269 pKa = 11.12 AYY271 pKa = 10.74 NSGQGSYY278 pKa = 7.47 WMEE281 pKa = 4.77 IIPEE285 pKa = 4.36 GDD287 pKa = 3.47 TVVLIDD293 pKa = 5.43 DD294 pKa = 3.99 IGVTAEE300 pKa = 4.15 RR301 pKa = 11.84 EE302 pKa = 3.46 IDD304 pKa = 3.6 ATSKK308 pKa = 11.15 ASDD311 pKa = 3.28 KK312 pKa = 11.26 SKK314 pKa = 11.13 SRR316 pKa = 11.84 DD317 pKa = 3.65 TNIKK321 pKa = 9.44 TSLIDD326 pKa = 3.31 SDD328 pKa = 3.98 NFIIKK333 pKa = 10.36 NIEE336 pKa = 3.75 EE337 pKa = 4.09
Molecular weight: 37.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.872
IPC_protein 3.897
Toseland 3.681
ProMoST 4.037
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.719
Grimsley 3.579
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.228
Thurlkill 3.719
EMBOSS 3.808
Sillero 4.012
Patrickios 1.901
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.91
Protein with the highest isoelectric point:
>tr|E3HBK3|E3HBK3_ILYPC Uncharacterized protein OS=Ilyobacter polytropus (strain ATCC 51220 / DSM 2926 / LMG 16218 / CuHBu1) OX=572544 GN=Ilyop_1928 PE=4 SV=1
MM1 pKa = 7.74 KK2 pKa = 10.3 KK3 pKa = 10.24 RR4 pKa = 11.84 LVLLLTLILIVSSVSFAATSSRR26 pKa = 11.84 RR27 pKa = 11.84 ATSGGWAVSSRR38 pKa = 11.84 RR39 pKa = 11.84 ATSGGWAVSSRR50 pKa = 11.84 RR51 pKa = 11.84 ATSGGWAVSSRR62 pKa = 11.84 RR63 pKa = 11.84 ATSGGWAVSSRR74 pKa = 11.84 RR75 pKa = 11.84 ATSGGWAVSSRR86 pKa = 11.84 RR87 pKa = 11.84 ATSGGWAVSSRR98 pKa = 11.84 RR99 pKa = 11.84 ATSGGWW105 pKa = 2.96
Molecular weight: 10.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.524
IPC2_protein 11.374
IPC_protein 12.983
Toseland 13.144
ProMoST 13.642
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.705
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.427
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.289
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2859
0
2859
873442
30
1489
305.5
34.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.802 ± 0.052
0.943 ± 0.019
5.464 ± 0.038
8.167 ± 0.049
4.631 ± 0.037
7.092 ± 0.042
1.473 ± 0.017
9.146 ± 0.046
9.398 ± 0.051
9.104 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.822 ± 0.021
5.088 ± 0.032
2.923 ± 0.025
2.102 ± 0.018
3.656 ± 0.031
6.222 ± 0.035
4.79 ± 0.03
6.542 ± 0.035
0.703 ± 0.014
3.936 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here