Proteome-pI 2.0 - Proteome Isoelectric Point Database

Ciona savignyi (Pacific transparent sea squirt)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Tunicata; Ascidiacea; Phlebobranchia; Cionidae; Ciona

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 20004 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|H2ZMH7|H2ZMH7_CIOSA Peptidase S1 domain-containing protein OS=Ciona savignyi OX=51511 PE=4 SV=1

MM1 pKa = 8.02CIRR4 pKa = 11.84LDD6 pKa = 3.77SDD8 pKa = 5.1DD9 pKa = 4.63DD10 pKa = 4.04TTSLSGLHH18 pKa = 6.54INLKK22 pKa = 8.35TLQAVSSMLAHH33 pKa = 6.02YY34 pKa = 10.14HH35 pKa = 6.82PIIQSDD41 pKa = 4.21CNGNPIFVAPPPPEE55 pKa = 5.44DD56 pKa = 4.3EE57 pKa = 4.38YY58 pKa = 11.47QSPEE62 pKa = 3.92GAPQSTSGRR71 pKa = 11.84AILDD75 pKa = 3.48STASRR80 pKa = 11.84DD81 pKa = 3.52STMTGDD87 pKa = 5.76DD88 pKa = 4.73DD89 pKa = 4.3YY90 pKa = 12.06QCALQEE96 pKa = 4.32LDD98 pKa = 5.39DD99 pKa = 4.71VIISQDD105 pKa = 3.09HH106 pKa = 5.97TLLSDD111 pKa = 4.29ALCEE115 pKa = 4.15LTDD118 pKa = 3.5VMEE121 pKa = 4.23KK122 pKa = 7.9MTYY125 pKa = 10.45FSAEE129 pKa = 4.07SVDD132 pKa = 5.12DD133 pKa = 3.69II134 pKa = 5.25

Molecular weight: 14.61 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|H2YR41|H2YR41_CIOSA CDI domain-containing protein OS=Ciona savignyi OX=51511 PE=3 SV=1

MM1 pKa = 7.69LNPPGPLGLAGSVGFGPVRR20 pKa = 11.84PPRR23 pKa = 11.84LMVNPPGPLGLVGSVGFGPVRR44 pKa = 11.84PPRR47 pKa = 11.84LKK49 pKa = 10.91LNPGPLGLAGSVGFGPVRR67 pKa = 11.84PPRR70 pKa = 11.84LMLNPPGPLGLAGSVGFGPVRR91 pKa = 11.84PPKK94 pKa = 10.58LKK96 pKa = 10.75LNPPGPLGLAGSVGFGPLRR115 pKa = 11.84PPRR118 pKa = 11.84LMLNPPGPLGLAGSVGFGPVRR139 pKa = 11.84PPRR142 pKa = 11.84LMLNPPGPLGLAGSVGFGPVRR163 pKa = 11.84PPRR166 pKa = 11.84LMLNPPGPLGLAGSVGFGPVRR187 pKa = 11.84PPRR190 pKa = 11.84LMLNPPGPLGLAGSVV205 pKa = 3.15

Molecular weight: 20.27 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
11592	8412	20004	9644551	22	18399	482.1	54.21

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
6.135 ± 0.014	2.424 ± 0.025	5.39 ± 0.014	6.076 ± 0.02	4.272 ± 0.015	6.114 ± 0.029	2.541 ± 0.011	5.733 ± 0.013	6.148 ± 0.021	9.079 ± 0.027

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.527 ± 0.009	4.878 ± 0.014	4.752 ± 0.019	4.047 ± 0.013	5.094 ± 0.015	7.724 ± 0.018	5.901 ± 0.018	6.633 ± 0.019	1.258 ± 0.007	3.272 ± 0.01

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski