Beihai partiti-like virus 2
Average proteome isoelectric point is 7.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLI2|A0A1L3KLI2_9VIRU RdRp OS=Beihai partiti-like virus 2 OX=1922504 PE=4 SV=1
MM1 pKa = 7.25 SLKK4 pKa = 10.3 RR5 pKa = 11.84 LPPSSSFPVDD15 pKa = 3.33 TRR17 pKa = 11.84 PPPVDD22 pKa = 3.74 QIALKK27 pKa = 10.08 YY28 pKa = 8.33 ARR30 pKa = 11.84 KK31 pKa = 8.89 WFGDD35 pKa = 3.79 GPVDD39 pKa = 3.46 KK40 pKa = 11.07 AIGSKK45 pKa = 10.01 HH46 pKa = 5.81 RR47 pKa = 11.84 AVASNEE53 pKa = 3.92 AIMDD57 pKa = 4.64 NIRR60 pKa = 11.84 GFDD63 pKa = 3.02 RR64 pKa = 11.84 RR65 pKa = 11.84 YY66 pKa = 10.31 APRR69 pKa = 11.84 ITDD72 pKa = 3.92 PVWEE76 pKa = 4.95 SILQRR81 pKa = 11.84 VYY83 pKa = 11.38 EE84 pKa = 4.22 EE85 pKa = 4.09 IKK87 pKa = 10.48 PNEE90 pKa = 4.27 LIIPYY95 pKa = 7.06 TTGGALKK102 pKa = 10.87 DD103 pKa = 3.65 PDD105 pKa = 4.16 FPMKK109 pKa = 10.36 KK110 pKa = 10.08 SPGLPWKK117 pKa = 10.83 LEE119 pKa = 4.22 GYY121 pKa = 7.14 GTKK124 pKa = 10.55 GDD126 pKa = 3.4 VCAIPEE132 pKa = 4.58 NINKK136 pKa = 9.46 IKK138 pKa = 10.34 EE139 pKa = 3.77 PWYY142 pKa = 9.52 KK143 pKa = 10.33 VGRR146 pKa = 11.84 GYY148 pKa = 8.46 HH149 pKa = 5.65 TSMPDD154 pKa = 2.88 CMVYY158 pKa = 10.23 YY159 pKa = 10.41 RR160 pKa = 11.84 SHH162 pKa = 6.98 ICDD165 pKa = 3.24 TEE167 pKa = 4.12 EE168 pKa = 3.76 NKK170 pKa = 9.61 IRR172 pKa = 11.84 AVWGYY177 pKa = 8.22 PLEE180 pKa = 4.44 VFTEE184 pKa = 4.46 EE185 pKa = 4.26 ARR187 pKa = 11.84 FVYY190 pKa = 10.07 PFLQHH195 pKa = 7.1 LKK197 pKa = 7.55 TTPEE201 pKa = 4.82 DD202 pKa = 3.59 YY203 pKa = 10.72 PIAYY207 pKa = 8.8 GLEE210 pKa = 4.19 MINGGMAYY218 pKa = 10.24 LEE220 pKa = 4.62 EE221 pKa = 4.65 AWQQTRR227 pKa = 11.84 AKK229 pKa = 9.46 FAIMLDD235 pKa = 3.19 WSSFDD240 pKa = 3.51 QSIPPWLIRR249 pKa = 11.84 DD250 pKa = 3.58 AFSILEE256 pKa = 3.96 RR257 pKa = 11.84 CFDD260 pKa = 4.16 FSHH263 pKa = 6.59 VLASDD268 pKa = 3.79 GKK270 pKa = 10.06 IFPVDD275 pKa = 3.11 PDD277 pKa = 3.2 KK278 pKa = 10.83 THH280 pKa = 6.81 RR281 pKa = 11.84 RR282 pKa = 11.84 WKK284 pKa = 10.38 RR285 pKa = 11.84 LISYY289 pKa = 8.04 FINTPFRR296 pKa = 11.84 MPTGEE301 pKa = 4.47 RR302 pKa = 11.84 FLKK305 pKa = 10.83 SGGVPSGSGFTNLIDD320 pKa = 4.24 SIVNILVVRR329 pKa = 11.84 YY330 pKa = 8.17 ITYY333 pKa = 10.43 HH334 pKa = 5.96 NLGEE338 pKa = 4.56 LPLHH342 pKa = 7.05 DD343 pKa = 4.66 MALGDD348 pKa = 4.33 DD349 pKa = 3.9 SVVYY353 pKa = 10.17 TNGHH357 pKa = 6.39 PSLEE361 pKa = 4.19 RR362 pKa = 11.84 MSEE365 pKa = 4.11 LAQEE369 pKa = 4.27 WFGMTINMRR378 pKa = 11.84 KK379 pKa = 9.87 SYY381 pKa = 7.92 VTRR384 pKa = 11.84 CARR387 pKa = 11.84 NIQFLGYY394 pKa = 8.45 YY395 pKa = 9.37 NEE397 pKa = 4.21 YY398 pKa = 10.78 GMPFRR403 pKa = 11.84 DD404 pKa = 3.78 VQFLIASWIFPEE416 pKa = 5.33 RR417 pKa = 11.84 FQTPDD422 pKa = 3.21 PAFTAIRR429 pKa = 11.84 GLGQLWSTMNSTAAVFWHH447 pKa = 6.05 YY448 pKa = 10.88 AVSDD452 pKa = 4.18 IISDD456 pKa = 3.98 YY457 pKa = 10.97 NLPSNWIEE465 pKa = 4.72 DD466 pKa = 3.46 IRR468 pKa = 11.84 EE469 pKa = 4.03 KK470 pKa = 10.94 YY471 pKa = 10.25 PNVLKK476 pKa = 10.55 FLSLYY481 pKa = 10.64 GITKK485 pKa = 10.13 ISLPEE490 pKa = 3.94 IDD492 pKa = 4.13 ARR494 pKa = 11.84 ASVFEE499 pKa = 3.93 VTPRR503 pKa = 11.84 IKK505 pKa = 10.35 PLRR508 pKa = 11.84 YY509 pKa = 8.9 PKK511 pKa = 10.44 RR512 pKa = 11.84 RR513 pKa = 11.84 DD514 pKa = 2.97 LDD516 pKa = 3.25 IRR518 pKa = 11.84 SCYY521 pKa = 9.18 QRR523 pKa = 11.84 GRR525 pKa = 11.84 DD526 pKa = 3.74 VVPLRR531 pKa = 11.84 DD532 pKa = 3.28
Molecular weight: 61.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.076
IPC2_protein 7.483
IPC_protein 7.424
Toseland 7.205
ProMoST 7.849
Dawson 8.083
Bjellqvist 8.302
Wikipedia 8.039
Rodwell 8.097
Grimsley 7.263
Solomon 8.17
Lehninger 8.185
Nozaki 8.361
DTASelect 8.156
Thurlkill 8.185
EMBOSS 8.302
Sillero 8.463
Patrickios 4.228
IPC_peptide 8.17
IPC2_peptide 7.337
IPC2.peptide.svr19 7.555
Protein with the highest isoelectric point:
>tr|A0A1L3KLI2|A0A1L3KLI2_9VIRU RdRp OS=Beihai partiti-like virus 2 OX=1922504 PE=4 SV=1
MM1 pKa = 7.57 EE2 pKa = 6.22 SIPEE6 pKa = 4.09 NSNPAAPEE14 pKa = 4.14 VPASAPRR21 pKa = 11.84 GGGYY25 pKa = 9.74 RR26 pKa = 11.84 GRR28 pKa = 11.84 GRR30 pKa = 11.84 SGPRR34 pKa = 11.84 GRR36 pKa = 11.84 RR37 pKa = 11.84 GRR39 pKa = 11.84 GGGGRR44 pKa = 11.84 GGQKK48 pKa = 9.79 PQTPPPPPAPAGGDD62 pKa = 3.31 PFAPRR67 pKa = 11.84 GKK69 pKa = 9.94 GGNPAIPKK77 pKa = 9.57 DD78 pKa = 3.9 DD79 pKa = 4.33 PAADD83 pKa = 3.7 SAIMIDD89 pKa = 4.38 GIAGFVEE96 pKa = 4.31 KK97 pKa = 10.08 TKK99 pKa = 11.05 NQLFQLDD106 pKa = 4.15 LSQYY110 pKa = 7.81 ITLVTEE116 pKa = 4.23 SYY118 pKa = 10.37 IAQTTCDD125 pKa = 3.11 RR126 pKa = 11.84 GLAKK130 pKa = 10.18 FVSASAYY137 pKa = 8.71 QYY139 pKa = 11.83 YY140 pKa = 8.77 MVILLWRR147 pKa = 11.84 RR148 pKa = 11.84 LFQLTSARR156 pKa = 11.84 RR157 pKa = 11.84 GDD159 pKa = 3.49 SCFDD163 pKa = 3.48 EE164 pKa = 5.24 LSRR167 pKa = 11.84 ALPHH171 pKa = 6.2 SLPVPKK177 pKa = 10.06 DD178 pKa = 2.96 IKK180 pKa = 10.67 IYY182 pKa = 10.66 LDD184 pKa = 3.29 SLGNIKK190 pKa = 10.61 DD191 pKa = 3.82 GEE193 pKa = 4.35 GQDD196 pKa = 3.22 WVFDD200 pKa = 5.07 FIADD204 pKa = 4.32 FSDD207 pKa = 3.19 TKK209 pKa = 11.2 VFGISGSYY217 pKa = 10.68 GKK219 pKa = 10.26 ISTEE223 pKa = 3.21 NHH225 pKa = 5.15 LQYY228 pKa = 10.2 EE229 pKa = 4.56 TVPCPVIPLLTMRR242 pKa = 11.84 ADD244 pKa = 3.27 ILRR247 pKa = 11.84 TKK249 pKa = 10.58 FAGTRR254 pKa = 11.84 SWSLPTALTPADD266 pKa = 4.12 VEE268 pKa = 4.45 DD269 pKa = 4.59 TSPGLPTLDD278 pKa = 3.32 LLGYY282 pKa = 10.13 QKK284 pKa = 11.22 AKK286 pKa = 10.91 NLTDD290 pKa = 3.36 DD291 pKa = 3.88 QYY293 pKa = 12.19 NVLIGNGVDD302 pKa = 3.43 VSGPAEE308 pKa = 4.47 DD309 pKa = 3.38 EE310 pKa = 4.49 TFFNARR316 pKa = 11.84 MVANVPVIQSLMGFLVGQFEE336 pKa = 4.66 STKK339 pKa = 10.56 CPMEE343 pKa = 5.55 RR344 pKa = 11.84 YY345 pKa = 7.72 DD346 pKa = 3.46 TTAITGSLAQVPFTVRR362 pKa = 11.84 SSFSFSPNEE371 pKa = 4.17 RR372 pKa = 11.84 ISQKK376 pKa = 10.84 VGITQTYY383 pKa = 7.93 RR384 pKa = 11.84 QSPPYY389 pKa = 8.86 VACASMMFRR398 pKa = 11.84 FRR400 pKa = 11.84 IMRR403 pKa = 11.84 AKK405 pKa = 10.45 KK406 pKa = 10.38 SGIQTDD412 pKa = 4.64 GFCYY416 pKa = 9.9 PWWNDD421 pKa = 3.0 TKK423 pKa = 10.59 KK424 pKa = 10.75 KK425 pKa = 10.81 YY426 pKa = 10.06 IIPPEE431 pKa = 4.4 WIANANDD438 pKa = 3.47 IFNKK442 pKa = 8.54 PEE444 pKa = 3.44 KK445 pKa = 10.04 WNISDD450 pKa = 4.2 FTAGTADD457 pKa = 3.64 GQALLSDD464 pKa = 4.78 LARR467 pKa = 11.84 KK468 pKa = 7.03 TKK470 pKa = 10.21 KK471 pKa = 10.05 KK472 pKa = 10.59 QEE474 pKa = 3.91
Molecular weight: 51.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.748
IPC2_protein 8.009
IPC_protein 7.907
Toseland 7.922
ProMoST 8.404
Dawson 8.682
Bjellqvist 8.814
Wikipedia 8.697
Rodwell 8.741
Grimsley 7.805
Solomon 8.799
Lehninger 8.785
Nozaki 8.931
DTASelect 8.624
Thurlkill 8.712
EMBOSS 8.858
Sillero 8.96
Patrickios 4.533
IPC_peptide 8.799
IPC2_peptide 7.556
IPC2.peptide.svr19 7.794
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1006
474
532
503.0
56.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.66 ± 0.9
1.193 ± 0.048
6.461 ± 0.052
4.672 ± 0.58
4.97 ± 0.078
7.455 ± 1.072
1.193 ± 0.511
6.859 ± 0.631
5.368 ± 0.218
7.058 ± 0.203
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.485 ± 0.249
3.579 ± 0.005
8.052 ± 0.117
3.28 ± 0.762
6.66 ± 0.499
6.958 ± 0.282
5.567 ± 0.645
5.07 ± 0.284
2.087 ± 0.405
4.374 ± 0.662
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here