Malassezia vespertilionis
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3791 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N1JBD8|A0A2N1JBD8_9BASI Uncharacterized protein OS=Malassezia vespertilionis OX=2020962 GN=MVES_002227 PE=4 SV=1
MM1 pKa = 7.6 SKK3 pKa = 8.94 TFYY6 pKa = 11.14 DD7 pKa = 4.27 EE8 pKa = 5.38 IEE10 pKa = 4.47 LEE12 pKa = 4.2 DD13 pKa = 3.74 MEE15 pKa = 4.93 FDD17 pKa = 3.34 EE18 pKa = 4.81 VNQVFHH24 pKa = 6.16 YY25 pKa = 8.44 PCPCGDD31 pKa = 3.45 RR32 pKa = 11.84 FEE34 pKa = 4.29 ITPLQLKK41 pKa = 10.14 NEE43 pKa = 4.15 EE44 pKa = 4.76 DD45 pKa = 3.19 IGRR48 pKa = 11.84 CPSCTLMIRR57 pKa = 11.84 IIFDD61 pKa = 3.92 PLDD64 pKa = 3.55 FTDD67 pKa = 5.3 IDD69 pKa = 4.23 DD70 pKa = 4.77 APHH73 pKa = 6.61 ATTQVAAAAA82 pKa = 4.2
Molecular weight: 9.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.773
IPC2_protein 3.961
IPC_protein 3.897
Toseland 3.706
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.732
Grimsley 3.617
Solomon 3.872
Lehninger 3.834
Nozaki 4.012
DTASelect 4.215
Thurlkill 3.757
EMBOSS 3.834
Sillero 4.024
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.91
Protein with the highest isoelectric point:
>tr|A0A2N1JDP7|A0A2N1JDP7_9BASI DLH domain-containing protein OS=Malassezia vespertilionis OX=2020962 GN=MVES_001315 PE=4 SV=1
MM1 pKa = 7.84 PFFGALTRR9 pKa = 11.84 GALFRR14 pKa = 11.84 APLRR18 pKa = 11.84 TFSSSAALGVAPRR31 pKa = 11.84 PAKK34 pKa = 10.94 VNTKK38 pKa = 10.44 LKK40 pKa = 8.17 THH42 pKa = 6.57 SGAKK46 pKa = 9.62 KK47 pKa = 10.06 RR48 pKa = 11.84 FFPMTGTGKK57 pKa = 10.4 GAAPLTKK64 pKa = 10.12 FKK66 pKa = 10.58 HH67 pKa = 4.42 GHH69 pKa = 5.86 ANKK72 pKa = 9.52 QHH74 pKa = 6.54 LNSAMSRR81 pKa = 11.84 VRR83 pKa = 11.84 LNRR86 pKa = 11.84 LEE88 pKa = 4.07 KK89 pKa = 10.5 SAVVSGGRR97 pKa = 11.84 TARR100 pKa = 11.84 MLRR103 pKa = 11.84 RR104 pKa = 11.84 LLAPRR109 pKa = 11.84 MM110 pKa = 3.85
Molecular weight: 11.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.461
IPC2_protein 11.008
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.232
Rodwell 12.515
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.237
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.078
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3791
0
3791
2125182
50
4829
560.6
61.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.911 ± 0.055
1.208 ± 0.014
5.707 ± 0.022
6.11 ± 0.034
3.428 ± 0.022
6.097 ± 0.029
2.932 ± 0.015
4.032 ± 0.026
4.013 ± 0.032
9.779 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.013
3.042 ± 0.021
5.934 ± 0.038
4.32 ± 0.023
6.676 ± 0.029
7.085 ± 0.037
5.652 ± 0.02
6.565 ± 0.024
1.301 ± 0.012
2.653 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here