Streptococcus satellite phage Javan109

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZFN7|A0A4D5ZFN7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan109 OX=2558526 GN=JavanS109_0008 PE=4 SV=1
MM1 pKa = 7.49TLTEE5 pKa = 5.24FIKK8 pKa = 10.89SHH10 pKa = 6.98DD11 pKa = 3.89KK12 pKa = 11.13AILAIDD18 pKa = 3.93RR19 pKa = 11.84LRR21 pKa = 11.84NLLGDD26 pKa = 3.63EE27 pKa = 4.41GFIGALDD34 pKa = 3.96FVADD38 pKa = 4.31IEE40 pKa = 4.37NHH42 pKa = 5.11MKK44 pKa = 10.25QADD47 pKa = 3.96FEE49 pKa = 4.57YY50 pKa = 10.28LDD52 pKa = 3.34WMQYY56 pKa = 10.61LATEE60 pKa = 4.51EE61 pKa = 4.07EE62 pKa = 4.52NEE64 pKa = 4.03

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZDM4|A0A4D5ZDM4_9VIRU Terminase small subunit OS=Streptococcus satellite phage Javan109 OX=2558526 GN=JavanS109_0005 PE=4 SV=1
MM1 pKa = 7.07AQAYY5 pKa = 6.51MTTGRR10 pKa = 11.84PGKK13 pKa = 9.93FGAVIRR19 pKa = 11.84WGQSRR24 pKa = 11.84LSKK27 pKa = 10.54RR28 pKa = 11.84SIVQGSRR35 pKa = 11.84QEE37 pKa = 3.88KK38 pKa = 10.42SLLITDD44 pKa = 4.36KK45 pKa = 10.88AHH47 pKa = 6.69KK48 pKa = 10.06GHH50 pKa = 5.62YY51 pKa = 7.46TEE53 pKa = 5.66KK54 pKa = 10.88NN55 pKa = 3.19

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23

0

23

3016

39

401

131.1

15.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.57 ± 0.522

0.431 ± 0.111

5.603 ± 0.325

8.123 ± 0.52

3.68 ± 0.345

4.874 ± 0.415

1.525 ± 0.252

8.886 ± 0.658

10.345 ± 0.498

9.98 ± 0.505

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.159

5.637 ± 0.344

2.42 ± 0.315

4.874 ± 0.269

4.31 ± 0.408

6.698 ± 0.53

5.04 ± 0.501

4.377 ± 0.452

0.995 ± 0.169

4.211 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski