Amino acid dipepetide frequency for Massilia sp. Root351

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.555AlaAla: 21.555 ± 0.198	1.32AlaCys: 1.32 ± 0.03	6.756AlaAsp: 6.756 ± 0.068	6.752AlaGlu: 6.752 ± 0.074	3.906AlaPhe: 3.906 ± 0.046	12.141AlaGly: 12.141 ± 0.096	2.556AlaHis: 2.556 ± 0.04	5.606AlaIle: 5.606 ± 0.059	4.055AlaLys: 4.055 ± 0.061	14.821AlaLeu: 14.821 ± 0.137	3.686AlaMet: 3.686 ± 0.047	3.375AlaAsn: 3.375 ± 0.049	6.925AlaPro: 6.925 ± 0.085	5.902AlaGln: 5.902 ± 0.062	8.624AlaArg: 8.624 ± 0.074	7.311AlaSer: 7.311 ± 0.058	6.034AlaThr: 6.034 ± 0.06	8.891AlaVal: 8.891 ± 0.068	1.823AlaTrp: 1.823 ± 0.034	2.963AlaTyr: 2.963 ± 0.037	0.002AlaXaa: 0.002 ± 0.001
Cys	1.202CysAla: 1.202 ± 0.024	0.126CysCys: 0.126 ± 0.009	0.465CysAsp: 0.465 ± 0.017	0.426CysGlu: 0.426 ± 0.016	0.289CysPhe: 0.289 ± 0.013	0.944CysGly: 0.944 ± 0.023	0.249CysHis: 0.249 ± 0.012	0.383CysIle: 0.383 ± 0.014	0.26CysLys: 0.26 ± 0.012	0.8CysLeu: 0.8 ± 0.019	0.195CysMet: 0.195 ± 0.01	0.237CysAsn: 0.237 ± 0.012	0.405CysPro: 0.405 ± 0.014	0.269CysGln: 0.269 ± 0.012	0.521CysArg: 0.521 ± 0.017	0.515CysSer: 0.515 ± 0.017	0.432CysThr: 0.432 ± 0.016	0.643CysVal: 0.643 ± 0.019	0.125CysTrp: 0.125 ± 0.007	0.237CysTyr: 0.237 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.859AspAla: 6.859 ± 0.061	0.448AspCys: 0.448 ± 0.016	2.637AspAsp: 2.637 ± 0.039	2.901AspGlu: 2.901 ± 0.046	2.045AspPhe: 2.045 ± 0.033	5.048AspGly: 5.048 ± 0.06	0.954AspHis: 0.954 ± 0.022	2.59AspIle: 2.59 ± 0.041	2.064AspLys: 2.064 ± 0.034	4.926AspLeu: 4.926 ± 0.054	1.335AspMet: 1.335 ± 0.025	1.451AspAsn: 1.451 ± 0.027	2.679AspPro: 2.679 ± 0.038	1.821AspGln: 1.821 ± 0.034	2.835AspArg: 2.835 ± 0.041	2.598AspSer: 2.598 ± 0.039	2.613AspThr: 2.613 ± 0.036	3.634AspVal: 3.634 ± 0.044	0.891AspTrp: 0.891 ± 0.023	1.605AspTyr: 1.605 ± 0.029	0.0AspXaa: 0.0 ± 0.0
Glu	6.28GluAla: 6.28 ± 0.072	0.356GluCys: 0.356 ± 0.014	2.158GluAsp: 2.158 ± 0.035	2.567GluGlu: 2.567 ± 0.039	1.806GluPhe: 1.806 ± 0.033	3.432GluGly: 3.432 ± 0.038	1.283GluHis: 1.283 ± 0.028	2.531GluIle: 2.531 ± 0.04	1.908GluLys: 1.908 ± 0.035	6.122GluLeu: 6.122 ± 0.055	1.252GluMet: 1.252 ± 0.029	1.34GluAsn: 1.34 ± 0.026	2.166GluPro: 2.166 ± 0.034	3.121GluGln: 3.121 ± 0.051	4.324GluArg: 4.324 ± 0.061	2.316GluSer: 2.316 ± 0.035	2.411GluThr: 2.411 ± 0.036	3.525GluVal: 3.525 ± 0.043	0.699GluTrp: 0.699 ± 0.018	1.194GluTyr: 1.194 ± 0.024	0.001GluXaa: 0.001 ± 0.001
Phe	4.043PheAla: 4.043 ± 0.046	0.353PheCys: 0.353 ± 0.015	2.359PheAsp: 2.359 ± 0.038	1.877PheGlu: 1.877 ± 0.03	1.284PhePhe: 1.284 ± 0.025	3.331PheGly: 3.331 ± 0.045	0.731PheHis: 0.731 ± 0.022	1.563PheIle: 1.563 ± 0.033	1.301PheLys: 1.301 ± 0.027	2.913PheLeu: 2.913 ± 0.037	0.846PheMet: 0.846 ± 0.022	1.285PheAsn: 1.285 ± 0.027	1.487PhePro: 1.487 ± 0.031	1.152PheGln: 1.152 ± 0.024	1.871PheArg: 1.871 ± 0.031	2.328PheSer: 2.328 ± 0.036	1.976PheThr: 1.976 ± 0.031	2.37PheVal: 2.37 ± 0.036	0.474PheTrp: 0.474 ± 0.016	0.976PheTyr: 0.976 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	10.649GlyAla: 10.649 ± 0.095	0.815GlyCys: 0.815 ± 0.022	3.98GlyAsp: 3.98 ± 0.042	4.174GlyGlu: 4.174 ± 0.048	3.109GlyPhe: 3.109 ± 0.039	7.542GlyGly: 7.542 ± 0.091	1.861GlyHis: 1.861 ± 0.034	3.946GlyIle: 3.946 ± 0.044	4.057GlyLys: 4.057 ± 0.05	8.427GlyLeu: 8.427 ± 0.076	2.542GlyMet: 2.542 ± 0.04	2.558GlyAsn: 2.558 ± 0.037	2.899GlyPro: 2.899 ± 0.04	3.469GlyGln: 3.469 ± 0.042	5.003GlyArg: 5.003 ± 0.054	4.922GlySer: 4.922 ± 0.066	4.326GlyThr: 4.326 ± 0.051	6.327GlyVal: 6.327 ± 0.055	1.365GlyTrp: 1.365 ± 0.029	2.67GlyTyr: 2.67 ± 0.038	0.001GlyXaa: 0.001 ± 0.001
His	2.85HisAla: 2.85 ± 0.04	0.257HisCys: 0.257 ± 0.011	1.127HisAsp: 1.127 ± 0.025	1.047HisGlu: 1.047 ± 0.02	0.892HisPhe: 0.892 ± 0.019	2.143HisGly: 2.143 ± 0.036	0.568HisHis: 0.568 ± 0.016	0.952HisIle: 0.952 ± 0.021	0.633HisLys: 0.633 ± 0.017	2.067HisLeu: 2.067 ± 0.036	0.499HisMet: 0.499 ± 0.017	0.569HisAsn: 0.569 ± 0.017	1.292HisPro: 1.292 ± 0.025	0.777HisGln: 0.777 ± 0.018	1.212HisArg: 1.212 ± 0.023	1.116HisSer: 1.116 ± 0.023	1.01HisThr: 1.01 ± 0.024	1.395HisVal: 1.395 ± 0.027	0.357HisTrp: 0.357 ± 0.014	0.698HisTyr: 0.698 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	6.231IleAla: 6.231 ± 0.067	0.42IleCys: 0.42 ± 0.015	2.989IleAsp: 2.989 ± 0.041	2.71IleGlu: 2.71 ± 0.038	1.315IlePhe: 1.315 ± 0.031	4.176IleGly: 4.176 ± 0.049	0.796IleHis: 0.796 ± 0.023	1.814IleIle: 1.814 ± 0.034	1.687IleLys: 1.687 ± 0.033	3.541IleLeu: 3.541 ± 0.044	0.865IleMet: 0.865 ± 0.021	1.573IleAsn: 1.573 ± 0.027	1.971IlePro: 1.971 ± 0.033	1.231IleGln: 1.231 ± 0.023	2.371IleArg: 2.371 ± 0.039	2.619IleSer: 2.619 ± 0.036	2.415IleThr: 2.415 ± 0.035	3.357IleVal: 3.357 ± 0.045	0.447IleTrp: 0.447 ± 0.016	1.053IleTyr: 1.053 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	4.284LysAla: 4.284 ± 0.062	0.17LysCys: 0.17 ± 0.011	1.82LysAsp: 1.82 ± 0.033	1.811LysGlu: 1.811 ± 0.037	1.094LysPhe: 1.094 ± 0.021	2.567LysGly: 2.567 ± 0.036	0.738LysHis: 0.738 ± 0.018	1.637LysIle: 1.637 ± 0.031	1.516LysLys: 1.516 ± 0.035	4.143LysLeu: 4.143 ± 0.048	1.015LysMet: 1.015 ± 0.022	1.209LysAsn: 1.209 ± 0.031	2.144LysPro: 2.144 ± 0.034	1.537LysGln: 1.537 ± 0.031	2.246LysArg: 2.246 ± 0.035	1.815LysSer: 1.815 ± 0.034	1.96LysThr: 1.96 ± 0.033	2.666LysVal: 2.666 ± 0.043	0.378LysTrp: 0.378 ± 0.014	0.822LysTyr: 0.822 ± 0.023	0.001LysXaa: 0.001 ± 0.001
Leu	15.621LeuAla: 15.621 ± 0.121	0.975LeuCys: 0.975 ± 0.02	5.789LeuAsp: 5.789 ± 0.058	5.271LeuGlu: 5.271 ± 0.06	3.443LeuPhe: 3.443 ± 0.048	8.065LeuGly: 8.065 ± 0.074	2.315LeuHis: 2.315 ± 0.031	4.007LeuIle: 4.007 ± 0.051	3.842LeuLys: 3.842 ± 0.046	11.316LeuLeu: 11.316 ± 0.131	2.429LeuMet: 2.429 ± 0.042	3.137LeuAsn: 3.137 ± 0.043	6.026LeuPro: 6.026 ± 0.056	4.172LeuGln: 4.172 ± 0.047	7.458LeuArg: 7.458 ± 0.073	6.289LeuSer: 6.289 ± 0.068	5.215LeuThr: 5.215 ± 0.059	6.879LeuVal: 6.879 ± 0.063	1.194LeuTrp: 1.194 ± 0.03	2.32LeuTyr: 2.32 ± 0.033	0.003LeuXaa: 0.003 ± 0.001
Met	3.246MetAla: 3.246 ± 0.043	0.165MetCys: 0.165 ± 0.009	1.244MetAsp: 1.244 ± 0.023	1.226MetGlu: 1.226 ± 0.025	0.757MetPhe: 0.757 ± 0.021	1.789MetGly: 1.789 ± 0.03	0.557MetHis: 0.557 ± 0.019	0.979MetIle: 0.979 ± 0.024	1.107MetLys: 1.107 ± 0.023	2.952MetLeu: 2.952 ± 0.039	0.689MetMet: 0.689 ± 0.02	0.913MetAsn: 0.913 ± 0.021	1.519MetPro: 1.519 ± 0.028	1.215MetGln: 1.215 ± 0.027	1.723MetArg: 1.723 ± 0.032	1.526MetSer: 1.526 ± 0.034	1.434MetThr: 1.434 ± 0.029	1.734MetVal: 1.734 ± 0.031	0.226MetTrp: 0.226 ± 0.011	0.469MetTyr: 0.469 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.717AsnAla: 3.717 ± 0.045	0.276AsnCys: 0.276 ± 0.012	1.469AsnAsp: 1.469 ± 0.03	1.291AsnGlu: 1.291 ± 0.027	1.085AsnPhe: 1.085 ± 0.026	2.842AsnGly: 2.842 ± 0.049	0.577AsnHis: 0.577 ± 0.017	1.446AsnIle: 1.446 ± 0.027	0.998AsnLys: 0.998 ± 0.028	2.956AsnLeu: 2.956 ± 0.04	0.729AsnMet: 0.729 ± 0.018	1.017AsnAsn: 1.017 ± 0.026	1.846AsnPro: 1.846 ± 0.03	1.059AsnGln: 1.059 ± 0.023	1.681AsnArg: 1.681 ± 0.025	1.56AsnSer: 1.56 ± 0.03	1.619AsnThr: 1.619 ± 0.029	2.211AsnVal: 2.211 ± 0.043	0.456AsnTrp: 0.456 ± 0.015	0.901AsnTyr: 0.901 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	7.88ProAla: 7.88 ± 0.085	0.331ProCys: 0.331 ± 0.012	3.064ProAsp: 3.064 ± 0.043	3.067ProGlu: 3.067 ± 0.039	1.7ProPhe: 1.7 ± 0.029	4.668ProGly: 4.668 ± 0.048	1.059ProHis: 1.059 ± 0.022	1.667ProIle: 1.667 ± 0.027	1.507ProLys: 1.507 ± 0.032	5.101ProLeu: 5.101 ± 0.057	1.192ProMet: 1.192 ± 0.025	1.348ProAsn: 1.348 ± 0.027	2.573ProPro: 2.573 ± 0.052	2.208ProGln: 2.208 ± 0.037	2.593ProArg: 2.593 ± 0.04	2.604ProSer: 2.604 ± 0.032	2.012ProThr: 2.012 ± 0.032	4.021ProVal: 4.021 ± 0.05	0.658ProTrp: 0.658 ± 0.018	1.228ProTyr: 1.228 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	5.946GlnAla: 5.946 ± 0.069	0.285GlnCys: 0.285 ± 0.012	1.778GlnAsp: 1.778 ± 0.032	1.843GlnGlu: 1.843 ± 0.034	1.44GlnPhe: 1.44 ± 0.031	3.089GlnGly: 3.089 ± 0.04	1.053GlnHis: 1.053 ± 0.02	1.768GlnIle: 1.768 ± 0.03	1.235GlnLys: 1.235 ± 0.024	4.681GlnLeu: 4.681 ± 0.05	1.049GlnMet: 1.049 ± 0.021	1.019GlnAsn: 1.019 ± 0.021	2.367GlnPro: 2.367 ± 0.037	2.367GlnGln: 2.367 ± 0.042	3.395GlnArg: 3.395 ± 0.046	1.989GlnSer: 1.989 ± 0.029	1.822GlnThr: 1.822 ± 0.03	3.023GlnVal: 3.023 ± 0.046	0.577GlnTrp: 0.577 ± 0.017	0.995GlnTyr: 0.995 ± 0.023	0.001GlnXaa: 0.001 ± 0.001
Arg	7.627ArgAla: 7.627 ± 0.073	0.542ArgCys: 0.542 ± 0.016	3.317ArgAsp: 3.317 ± 0.041	3.582ArgGlu: 3.582 ± 0.046	2.468ArgPhe: 2.468 ± 0.036	4.306ArgGly: 4.306 ± 0.039	1.724ArgHis: 1.724 ± 0.035	3.219ArgIle: 3.219 ± 0.033	2.302ArgLys: 2.302 ± 0.031	7.067ArgLeu: 7.067 ± 0.074	1.894ArgMet: 1.894 ± 0.029	2.031ArgAsn: 2.031 ± 0.032	2.841ArgPro: 2.841 ± 0.045	2.977ArgGln: 2.977 ± 0.046	4.432ArgArg: 4.432 ± 0.054	3.524ArgSer: 3.524 ± 0.042	2.978ArgThr: 2.978 ± 0.043	4.391ArgVal: 4.391 ± 0.049	0.977ArgTrp: 0.977 ± 0.027	2.116ArgTyr: 2.116 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	7.292SerAla: 7.292 ± 0.061	0.444SerCys: 0.444 ± 0.014	2.72SerAsp: 2.72 ± 0.037	2.528SerGlu: 2.528 ± 0.037	2.116SerPhe: 2.116 ± 0.034	5.736SerGly: 5.736 ± 0.072	1.163SerHis: 1.163 ± 0.024	2.512SerIle: 2.512 ± 0.041	1.858SerLys: 1.858 ± 0.034	5.612SerLeu: 5.612 ± 0.055	1.398SerMet: 1.398 ± 0.025	1.657SerAsn: 1.657 ± 0.029	2.672SerPro: 2.672 ± 0.037	1.938SerGln: 1.938 ± 0.036	3.263SerArg: 3.263 ± 0.047	3.275SerSer: 3.275 ± 0.05	2.913SerThr: 2.913 ± 0.052	4.068SerVal: 4.068 ± 0.056	0.802SerTrp: 0.802 ± 0.021	1.665SerTyr: 1.665 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	6.263ThrAla: 6.263 ± 0.072	0.378ThrCys: 0.378 ± 0.012	2.36ThrAsp: 2.36 ± 0.031	2.241ThrGlu: 2.241 ± 0.033	1.681ThrPhe: 1.681 ± 0.027	4.625ThrGly: 4.625 ± 0.054	0.982ThrHis: 0.982 ± 0.021	2.257ThrIle: 2.257 ± 0.035	1.296ThrLys: 1.296 ± 0.028	5.998ThrLeu: 5.998 ± 0.06	1.16ThrMet: 1.16 ± 0.025	1.298ThrAsn: 1.298 ± 0.029	3.181ThrPro: 3.181 ± 0.042	1.745ThrGln: 1.745 ± 0.027	2.974ThrArg: 2.974 ± 0.043	2.7ThrSer: 2.7 ± 0.049	2.512ThrThr: 2.512 ± 0.052	4.292ThrVal: 4.292 ± 0.052	0.6ThrTrp: 0.6 ± 0.021	1.237ThrTyr: 1.237 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	9.192ValAla: 9.192 ± 0.081	0.653ValCys: 0.653 ± 0.017	3.749ValAsp: 3.749 ± 0.045	3.69ValGlu: 3.69 ± 0.042	2.521ValPhe: 2.521 ± 0.033	5.02ValGly: 5.02 ± 0.053	1.451ValHis: 1.451 ± 0.025	3.132ValIle: 3.132 ± 0.04	2.573ValLys: 2.573 ± 0.044	7.869ValLeu: 7.869 ± 0.07	1.757ValMet: 1.757 ± 0.033	2.379ValAsn: 2.379 ± 0.041	3.636ValPro: 3.636 ± 0.045	2.852ValGln: 2.852 ± 0.035	4.742ValArg: 4.742 ± 0.045	4.234ValSer: 4.234 ± 0.054	3.941ValThr: 3.941 ± 0.053	5.356ValVal: 5.356 ± 0.06	0.838ValTrp: 0.838 ± 0.02	1.72ValTyr: 1.72 ± 0.027	0.001ValXaa: 0.001 ± 0.001
Trp	1.18TrpAla: 1.18 ± 0.027	0.133TrpCys: 0.133 ± 0.009	0.668TrpAsp: 0.668 ± 0.017	0.552TrpGlu: 0.552 ± 0.016	0.512TrpPhe: 0.512 ± 0.017	0.878TrpGly: 0.878 ± 0.021	0.323TrpHis: 0.323 ± 0.012	0.596TrpIle: 0.596 ± 0.017	0.492TrpLys: 0.492 ± 0.017	1.823TrpLeu: 1.823 ± 0.039	0.412TrpMet: 0.412 ± 0.016	0.512TrpAsn: 0.512 ± 0.016	0.594TrpPro: 0.594 ± 0.019	0.782TrpGln: 0.782 ± 0.021	1.143TrpArg: 1.143 ± 0.024	0.808TrpSer: 0.808 ± 0.025	0.709TrpThr: 0.709 ± 0.02	0.815TrpVal: 0.815 ± 0.021	0.228TrpTrp: 0.228 ± 0.011	0.361TrpTyr: 0.361 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.087TyrAla: 3.087 ± 0.048	0.275TyrCys: 0.275 ± 0.011	1.485TyrAsp: 1.485 ± 0.028	1.233TyrGlu: 1.233 ± 0.026	1.06TyrPhe: 1.06 ± 0.022	2.298TyrGly: 2.298 ± 0.034	0.52TyrHis: 0.52 ± 0.015	0.977TyrIle: 0.977 ± 0.025	0.879TyrLys: 0.879 ± 0.021	2.628TyrLeu: 2.628 ± 0.038	0.524TyrMet: 0.524 ± 0.014	0.828TyrAsn: 0.828 ± 0.022	1.257TyrPro: 1.257 ± 0.028	1.127TyrGln: 1.127 ± 0.021	1.904TyrArg: 1.904 ± 0.03	1.585TyrSer: 1.585 ± 0.032	1.449TyrThr: 1.449 ± 0.03	1.705TyrVal: 1.705 ± 0.031	0.405TyrTrp: 0.405 ± 0.013	0.802TyrTyr: 0.802 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6059 proteins (2134414 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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