Fusarium poae mitovirus 3
Average proteome isoelectric point is 7.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4ZA45|A0A1B4ZA45_9VIRU RNA-dependent RNA polymerase OS=Fusarium poae mitovirus 3 OX=1848152 PE=4 SV=1
MM1 pKa = 7.39 KK2 pKa = 10.24 AIKK5 pKa = 10.19 FILNFKK11 pKa = 9.63 LKK13 pKa = 10.5 QIIRR17 pKa = 11.84 DD18 pKa = 3.73 SILPVGKK25 pKa = 10.01 DD26 pKa = 3.49 FNKK29 pKa = 10.34 KK30 pKa = 9.79 CFPRR34 pKa = 11.84 LLNHH38 pKa = 6.11 LRR40 pKa = 11.84 KK41 pKa = 9.63 IYY43 pKa = 10.16 FIVFDD48 pKa = 4.34 DD49 pKa = 5.29 FKK51 pKa = 11.16 AIRR54 pKa = 11.84 IRR56 pKa = 11.84 DD57 pKa = 3.9 RR58 pKa = 11.84 LVMTNNFLQFVSKK71 pKa = 10.18 MMRR74 pKa = 11.84 NHH76 pKa = 5.4 GTVFTVKK83 pKa = 8.98 WLKK86 pKa = 10.87 ACTVCLQKK94 pKa = 10.48 WLGEE98 pKa = 4.1 DD99 pKa = 3.42 TVKK102 pKa = 10.43 SLRR105 pKa = 11.84 EE106 pKa = 3.98 IEE108 pKa = 4.7 PGLPLPRR115 pKa = 11.84 VINGCPAIINRR126 pKa = 11.84 HH127 pKa = 5.07 DD128 pKa = 4.01 RR129 pKa = 11.84 KK130 pKa = 10.72 LMRR133 pKa = 11.84 AGNKK137 pKa = 9.66 HH138 pKa = 6.96 IIRR141 pKa = 11.84 YY142 pKa = 4.64 WHH144 pKa = 6.45 SLFSIYY150 pKa = 10.01 RR151 pKa = 11.84 VLKK154 pKa = 10.2 IPGKK158 pKa = 10.19 IKK160 pKa = 10.86 LEE162 pKa = 4.52 TITAPYY168 pKa = 9.02 KK169 pKa = 10.95 GSVDD173 pKa = 3.71 YY174 pKa = 11.28 LNKK177 pKa = 10.42 CLEE180 pKa = 3.99 ISLNTKK186 pKa = 8.14 WQASIQKK193 pKa = 9.21 ICQNHH198 pKa = 5.6 NLAPTTFHH206 pKa = 6.64 MSGKK210 pKa = 10.18 ASPSNVNSSTGILSDD225 pKa = 3.42 IYY227 pKa = 11.32 YY228 pKa = 10.93 LLAHH232 pKa = 7.4 PDD234 pKa = 3.37 GAFVFSNILKK244 pKa = 9.56 HH245 pKa = 6.81 LEE247 pKa = 3.9 IVGSVWNTQSFLQRR261 pKa = 11.84 LNDD264 pKa = 3.45 GAEE267 pKa = 3.79 IVKK270 pKa = 10.26 RR271 pKa = 11.84 MSSIQTKK278 pKa = 10.24 CSMRR282 pKa = 11.84 NPFGQFAIKK291 pKa = 10.18 EE292 pKa = 4.03 EE293 pKa = 4.1 AAGKK297 pKa = 9.98 VRR299 pKa = 11.84 VFALVDD305 pKa = 4.18 SITQSVMKK313 pKa = 9.49 PIHH316 pKa = 6.42 LGLFKK321 pKa = 10.8 VLRR324 pKa = 11.84 HH325 pKa = 6.04 LPNDD329 pKa = 3.56 GTFDD333 pKa = 3.22 QDD335 pKa = 3.88 ASVTRR340 pKa = 11.84 CSEE343 pKa = 4.04 KK344 pKa = 10.68 ASEE347 pKa = 4.09 AGKK350 pKa = 10.59 AFSFDD355 pKa = 3.22 LSAATDD361 pKa = 3.88 RR362 pKa = 11.84 LPVSLTGNIIEE373 pKa = 4.88 SLFKK377 pKa = 10.68 IPGLSQSWQKK387 pKa = 11.36 VMVDD391 pKa = 3.05 RR392 pKa = 11.84 NFRR395 pKa = 11.84 FPLNIIEE402 pKa = 4.8 NYY404 pKa = 10.16 DD405 pKa = 3.51 IEE407 pKa = 6.97 DD408 pKa = 2.91 IDD410 pKa = 3.55 YY411 pKa = 9.69 RR412 pKa = 11.84 YY413 pKa = 10.74 SVGQPMGCLSSWAGLAITHH432 pKa = 6.13 HH433 pKa = 7.41 WIMQLCSYY441 pKa = 10.48 LVTDD445 pKa = 3.02 SWDD448 pKa = 3.03 WEE450 pKa = 3.91 EE451 pKa = 4.57 RR452 pKa = 11.84 YY453 pKa = 10.17 EE454 pKa = 4.8 ILGDD458 pKa = 4.15 DD459 pKa = 3.18 IVIFDD464 pKa = 4.29 EE465 pKa = 4.57 EE466 pKa = 4.32 LANTYY471 pKa = 9.71 LQVMEE476 pKa = 4.58 WLGLDD481 pKa = 3.17 INLSKK486 pKa = 11.01 SIISKK491 pKa = 9.99 NKK493 pKa = 7.78 PTFEE497 pKa = 3.8 FAKK500 pKa = 9.3 RR501 pKa = 11.84 TFSGGSLVSGITGSQINSCTSLSSRR526 pKa = 11.84 VNSVYY531 pKa = 10.9 SWIKK535 pKa = 10.42 LGYY538 pKa = 9.84 LNNLEE543 pKa = 4.61 TITIVLNKK551 pKa = 9.93 FNTKK555 pKa = 9.92 FSYY558 pKa = 11.04 KK559 pKa = 10.29 DD560 pKa = 3.56 FSLSASAFSLLGLCKK575 pKa = 10.51 NIEE578 pKa = 4.2 HH579 pKa = 6.98 KK580 pKa = 10.51 IIMSSLVNPSKK591 pKa = 11.03 GCLWDD596 pKa = 3.62 MEE598 pKa = 4.65 TEE600 pKa = 4.05 NFQVPTRR607 pKa = 11.84 TLLTMARR614 pKa = 11.84 DD615 pKa = 4.7 LITKK619 pKa = 10.11 GEE621 pKa = 4.33 SEE623 pKa = 4.18 VTLPRR628 pKa = 11.84 MEE630 pKa = 4.54 GRR632 pKa = 11.84 EE633 pKa = 3.84 EE634 pKa = 3.75 WFEE637 pKa = 3.86 EE638 pKa = 4.3 SEE640 pKa = 4.16 QLIVAGILQRR650 pKa = 11.84 ALYY653 pKa = 8.98 RR654 pKa = 11.84 SRR656 pKa = 11.84 MLSLKK661 pKa = 9.98 YY662 pKa = 10.13 EE663 pKa = 3.95 EE664 pKa = 5.39 SVRR667 pKa = 11.84 EE668 pKa = 3.72 WAIDD672 pKa = 3.57 LGHH675 pKa = 7.32 LKK677 pKa = 10.7 FKK679 pKa = 10.52 DD680 pKa = 3.63 DD681 pKa = 4.91 RR682 pKa = 11.84 EE683 pKa = 4.19 LLPLIEE689 pKa = 4.87 GWLLDD694 pKa = 3.69 ALIDD698 pKa = 3.96 NRR700 pKa = 11.84 SSAATDD706 pKa = 3.57 PFEE709 pKa = 6.83 LEE711 pKa = 4.4 DD712 pKa = 4.14 KK713 pKa = 11.03 VEE715 pKa = 4.2 KK716 pKa = 10.74 LLTYY720 pKa = 9.69 HH721 pKa = 7.33 AKK723 pKa = 8.69 TNNVILSKK731 pKa = 10.5 AYY733 pKa = 9.72 EE734 pKa = 3.94 LLQEE738 pKa = 4.29 VEE740 pKa = 4.18 NLEE743 pKa = 4.09 YY744 pKa = 10.53 LYY746 pKa = 10.58 KK747 pKa = 10.58 QPTKK751 pKa = 10.55 KK752 pKa = 10.22 SQQLYY757 pKa = 10.85 DD758 pKa = 3.44 RR759 pKa = 11.84 LSNKK763 pKa = 7.23 TLRR766 pKa = 11.84 DD767 pKa = 2.87 ITTPFFMSGPEE778 pKa = 4.07 YY779 pKa = 10.24 WSVPSPNNFNN789 pKa = 3.55
Molecular weight: 90.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.785
IPC2_protein 8.024
IPC_protein 7.849
Toseland 7.863
ProMoST 8.448
Dawson 8.712
Bjellqvist 8.858
Wikipedia 8.712
Rodwell 8.799
Grimsley 7.878
Solomon 8.77
Lehninger 8.77
Nozaki 9.048
DTASelect 8.668
Thurlkill 8.756
EMBOSS 8.902
Sillero 9.019
Patrickios 4.584
IPC_peptide 8.77
IPC2_peptide 7.585
IPC2.peptide.svr19 7.823
Protein with the highest isoelectric point:
>tr|A0A1B4ZA45|A0A1B4ZA45_9VIRU RNA-dependent RNA polymerase OS=Fusarium poae mitovirus 3 OX=1848152 PE=4 SV=1
MM1 pKa = 7.39 KK2 pKa = 10.24 AIKK5 pKa = 10.19 FILNFKK11 pKa = 9.63 LKK13 pKa = 10.5 QIIRR17 pKa = 11.84 DD18 pKa = 3.73 SILPVGKK25 pKa = 10.01 DD26 pKa = 3.49 FNKK29 pKa = 10.34 KK30 pKa = 9.79 CFPRR34 pKa = 11.84 LLNHH38 pKa = 6.11 LRR40 pKa = 11.84 KK41 pKa = 9.63 IYY43 pKa = 10.16 FIVFDD48 pKa = 4.34 DD49 pKa = 5.29 FKK51 pKa = 11.16 AIRR54 pKa = 11.84 IRR56 pKa = 11.84 DD57 pKa = 3.9 RR58 pKa = 11.84 LVMTNNFLQFVSKK71 pKa = 10.18 MMRR74 pKa = 11.84 NHH76 pKa = 5.4 GTVFTVKK83 pKa = 8.98 WLKK86 pKa = 10.87 ACTVCLQKK94 pKa = 10.48 WLGEE98 pKa = 4.1 DD99 pKa = 3.42 TVKK102 pKa = 10.43 SLRR105 pKa = 11.84 EE106 pKa = 3.98 IEE108 pKa = 4.7 PGLPLPRR115 pKa = 11.84 VINGCPAIINRR126 pKa = 11.84 HH127 pKa = 5.07 DD128 pKa = 4.01 RR129 pKa = 11.84 KK130 pKa = 10.72 LMRR133 pKa = 11.84 AGNKK137 pKa = 9.66 HH138 pKa = 6.96 IIRR141 pKa = 11.84 YY142 pKa = 4.64 WHH144 pKa = 6.45 SLFSIYY150 pKa = 10.01 RR151 pKa = 11.84 VLKK154 pKa = 10.2 IPGKK158 pKa = 10.19 IKK160 pKa = 10.86 LEE162 pKa = 4.52 TITAPYY168 pKa = 9.02 KK169 pKa = 10.95 GSVDD173 pKa = 3.71 YY174 pKa = 11.28 LNKK177 pKa = 10.42 CLEE180 pKa = 3.99 ISLNTKK186 pKa = 8.14 WQASIQKK193 pKa = 9.21 ICQNHH198 pKa = 5.6 NLAPTTFHH206 pKa = 6.64 MSGKK210 pKa = 10.18 ASPSNVNSSTGILSDD225 pKa = 3.42 IYY227 pKa = 11.32 YY228 pKa = 10.93 LLAHH232 pKa = 7.4 PDD234 pKa = 3.37 GAFVFSNILKK244 pKa = 9.56 HH245 pKa = 6.81 LEE247 pKa = 3.9 IVGSVWNTQSFLQRR261 pKa = 11.84 LNDD264 pKa = 3.45 GAEE267 pKa = 3.79 IVKK270 pKa = 10.26 RR271 pKa = 11.84 MSSIQTKK278 pKa = 10.24 CSMRR282 pKa = 11.84 NPFGQFAIKK291 pKa = 10.18 EE292 pKa = 4.03 EE293 pKa = 4.1 AAGKK297 pKa = 9.98 VRR299 pKa = 11.84 VFALVDD305 pKa = 4.18 SITQSVMKK313 pKa = 9.49 PIHH316 pKa = 6.42 LGLFKK321 pKa = 10.8 VLRR324 pKa = 11.84 HH325 pKa = 6.04 LPNDD329 pKa = 3.56 GTFDD333 pKa = 3.22 QDD335 pKa = 3.88 ASVTRR340 pKa = 11.84 CSEE343 pKa = 4.04 KK344 pKa = 10.68 ASEE347 pKa = 4.09 AGKK350 pKa = 10.59 AFSFDD355 pKa = 3.22 LSAATDD361 pKa = 3.88 RR362 pKa = 11.84 LPVSLTGNIIEE373 pKa = 4.88 SLFKK377 pKa = 10.68 IPGLSQSWQKK387 pKa = 11.36 VMVDD391 pKa = 3.05 RR392 pKa = 11.84 NFRR395 pKa = 11.84 FPLNIIEE402 pKa = 4.8 NYY404 pKa = 10.16 DD405 pKa = 3.51 IEE407 pKa = 6.97 DD408 pKa = 2.91 IDD410 pKa = 3.55 YY411 pKa = 9.69 RR412 pKa = 11.84 YY413 pKa = 10.74 SVGQPMGCLSSWAGLAITHH432 pKa = 6.13 HH433 pKa = 7.41 WIMQLCSYY441 pKa = 10.48 LVTDD445 pKa = 3.02 SWDD448 pKa = 3.03 WEE450 pKa = 3.91 EE451 pKa = 4.57 RR452 pKa = 11.84 YY453 pKa = 10.17 EE454 pKa = 4.8 ILGDD458 pKa = 4.15 DD459 pKa = 3.18 IVIFDD464 pKa = 4.29 EE465 pKa = 4.57 EE466 pKa = 4.32 LANTYY471 pKa = 9.71 LQVMEE476 pKa = 4.58 WLGLDD481 pKa = 3.17 INLSKK486 pKa = 11.01 SIISKK491 pKa = 9.99 NKK493 pKa = 7.78 PTFEE497 pKa = 3.8 FAKK500 pKa = 9.3 RR501 pKa = 11.84 TFSGGSLVSGITGSQINSCTSLSSRR526 pKa = 11.84 VNSVYY531 pKa = 10.9 SWIKK535 pKa = 10.42 LGYY538 pKa = 9.84 LNNLEE543 pKa = 4.61 TITIVLNKK551 pKa = 9.93 FNTKK555 pKa = 9.92 FSYY558 pKa = 11.04 KK559 pKa = 10.29 DD560 pKa = 3.56 FSLSASAFSLLGLCKK575 pKa = 10.51 NIEE578 pKa = 4.2 HH579 pKa = 6.98 KK580 pKa = 10.51 IIMSSLVNPSKK591 pKa = 11.03 GCLWDD596 pKa = 3.62 MEE598 pKa = 4.65 TEE600 pKa = 4.05 NFQVPTRR607 pKa = 11.84 TLLTMARR614 pKa = 11.84 DD615 pKa = 4.7 LITKK619 pKa = 10.11 GEE621 pKa = 4.33 SEE623 pKa = 4.18 VTLPRR628 pKa = 11.84 MEE630 pKa = 4.54 GRR632 pKa = 11.84 EE633 pKa = 3.84 EE634 pKa = 3.75 WFEE637 pKa = 3.86 EE638 pKa = 4.3 SEE640 pKa = 4.16 QLIVAGILQRR650 pKa = 11.84 ALYY653 pKa = 8.98 RR654 pKa = 11.84 SRR656 pKa = 11.84 MLSLKK661 pKa = 9.98 YY662 pKa = 10.13 EE663 pKa = 3.95 EE664 pKa = 5.39 SVRR667 pKa = 11.84 EE668 pKa = 3.72 WAIDD672 pKa = 3.57 LGHH675 pKa = 7.32 LKK677 pKa = 10.7 FKK679 pKa = 10.52 DD680 pKa = 3.63 DD681 pKa = 4.91 RR682 pKa = 11.84 EE683 pKa = 4.19 LLPLIEE689 pKa = 4.87 GWLLDD694 pKa = 3.69 ALIDD698 pKa = 3.96 NRR700 pKa = 11.84 SSAATDD706 pKa = 3.57 PFEE709 pKa = 6.83 LEE711 pKa = 4.4 DD712 pKa = 4.14 KK713 pKa = 11.03 VEE715 pKa = 4.2 KK716 pKa = 10.74 LLTYY720 pKa = 9.69 HH721 pKa = 7.33 AKK723 pKa = 8.69 TNNVILSKK731 pKa = 10.5 AYY733 pKa = 9.72 EE734 pKa = 3.94 LLQEE738 pKa = 4.29 VEE740 pKa = 4.18 NLEE743 pKa = 4.09 YY744 pKa = 10.53 LYY746 pKa = 10.58 KK747 pKa = 10.58 QPTKK751 pKa = 10.55 KK752 pKa = 10.22 SQQLYY757 pKa = 10.85 DD758 pKa = 3.44 RR759 pKa = 11.84 LSNKK763 pKa = 7.23 TLRR766 pKa = 11.84 DD767 pKa = 2.87 ITTPFFMSGPEE778 pKa = 4.07 YY779 pKa = 10.24 WSVPSPNNFNN789 pKa = 3.55
Molecular weight: 90.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.785
IPC2_protein 8.024
IPC_protein 7.849
Toseland 7.863
ProMoST 8.448
Dawson 8.712
Bjellqvist 8.858
Wikipedia 8.712
Rodwell 8.799
Grimsley 7.878
Solomon 8.77
Lehninger 8.77
Nozaki 9.048
DTASelect 8.668
Thurlkill 8.756
EMBOSS 8.902
Sillero 9.019
Patrickios 4.584
IPC_peptide 8.77
IPC2_peptide 7.585
IPC2.peptide.svr19 7.823
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
789
789
789
789.0
90.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.689 ± 0.0
1.648 ± 0.0
4.943 ± 0.0
5.83 ± 0.0
5.07 ± 0.0
4.816 ± 0.0
2.028 ± 0.0
7.858 ± 0.0
7.351 ± 0.0
11.407 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.408 ± 0.0
5.577 ± 0.0
3.676 ± 0.0
3.169 ± 0.0
4.943 ± 0.0
8.872 ± 0.0
5.196 ± 0.0
5.323 ± 0.0
2.155 ± 0.0
3.042 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here