Mycobacterium phage Duke13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Omegavirus; unclassified Omegavirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 239 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9UB58|A0A3S9UB58_9CAUD Uncharacterized protein OS=Mycobacterium phage Duke13 OX=2499038 GN=184 PE=4 SV=1
MM1 pKa = 7.45SATADD6 pKa = 3.69TLVSAFADD14 pKa = 3.47LGEE17 pKa = 4.64PLDD20 pKa = 5.63DD21 pKa = 4.35EE22 pKa = 5.07ANCYY26 pKa = 10.75LEE28 pKa = 4.47VDD30 pKa = 3.71TEE32 pKa = 4.05EE33 pKa = 4.1RR34 pKa = 11.84QAIIVWVEE42 pKa = 3.24PLAFHH47 pKa = 7.63AEE49 pKa = 4.41FNGPRR54 pKa = 11.84QYY56 pKa = 11.42VATSSYY62 pKa = 10.41FVDD65 pKa = 4.18RR66 pKa = 11.84AMPDD70 pKa = 2.63HH71 pKa = 7.1WSTHH75 pKa = 4.29VDD77 pKa = 2.85ADD79 pKa = 3.39GWLTRR84 pKa = 11.84AVADD88 pKa = 4.69AIVARR93 pKa = 11.84LQGCIGARR101 pKa = 11.84GEE103 pKa = 4.29VYY105 pKa = 10.02EE106 pKa = 4.57LEE108 pKa = 4.42YY109 pKa = 11.32GGDD112 pKa = 3.55EE113 pKa = 4.04PNITFEE119 pKa = 4.3IVTSYY124 pKa = 11.56EE125 pKa = 3.87DD126 pKa = 4.98GEE128 pKa = 4.77TFDD131 pKa = 3.78HH132 pKa = 6.5WFDD135 pKa = 3.49RR136 pKa = 11.84VGWPIVATLRR146 pKa = 11.84NVTDD150 pKa = 4.35PGTFMSPYY158 pKa = 8.77LFAMASLEE166 pKa = 4.2VEE168 pKa = 4.43SS169 pKa = 4.53

Molecular weight: 18.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9UAY8|A0A3S9UAY8_9CAUD HNHc domain-containing protein OS=Mycobacterium phage Duke13 OX=2499038 GN=133 PE=4 SV=1
MM1 pKa = 6.49THH3 pKa = 7.07RR4 pKa = 11.84GLEE7 pKa = 3.97RR8 pKa = 11.84AEE10 pKa = 3.92EE11 pKa = 3.95RR12 pKa = 11.84RR13 pKa = 11.84VQIAKK18 pKa = 10.48LIRR21 pKa = 11.84QGLTNKK27 pKa = 9.43EE28 pKa = 3.79VAQIVGVTDD37 pKa = 3.29RR38 pKa = 11.84TVNRR42 pKa = 11.84ARR44 pKa = 11.84EE45 pKa = 4.02RR46 pKa = 11.84KK47 pKa = 9.19GLKK50 pKa = 9.77KK51 pKa = 9.73PQPPRR56 pKa = 11.84ISEE59 pKa = 4.07EE60 pKa = 4.17TLSAGYY66 pKa = 10.86QMLLDD71 pKa = 4.0GCSYY75 pKa = 11.69AEE77 pKa = 3.99VARR80 pKa = 11.84TLGHH84 pKa = 6.73SRR86 pKa = 11.84GAWRR90 pKa = 11.84KK91 pKa = 9.21KK92 pKa = 10.09FPGFSWSHH100 pKa = 4.68VEE102 pKa = 3.73CGRR105 pKa = 11.84FRR107 pKa = 11.84KK108 pKa = 9.42LQEE111 pKa = 4.09RR112 pKa = 11.84YY113 pKa = 9.93EE114 pKa = 4.26GLLL117 pKa = 3.55

Molecular weight: 13.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

239

0

239

34928

22

1636

146.1

16.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.737 ± 0.255

1.46 ± 0.14

6.399 ± 0.153

6.831 ± 0.237

3.238 ± 0.113

8.741 ± 0.416

2.316 ± 0.123

4.75 ± 0.137

4.031 ± 0.168

8.102 ± 0.163

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.422 ± 0.088

3.37 ± 0.117

5.471 ± 0.14

3.172 ± 0.139

6.585 ± 0.243

5.483 ± 0.149

5.646 ± 0.186

6.908 ± 0.221

2.313 ± 0.109

3.023 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski