Macellibacteroides sp. HH-ZS

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Porphyromonadaceae; Macellibacteroides; unclassified Macellibacteroides

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3239 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C2BTL3|A0A1C2BTL3_9PORP Transcriptional regulator OS=Macellibacteroides sp. HH-ZS OX=1857568 GN=A9168_10550 PE=4 SV=1
MM1 pKa = 7.39SIKK4 pKa = 10.34RR5 pKa = 11.84VWVEE9 pKa = 4.58DD10 pKa = 3.58DD11 pKa = 4.65CISCGSCEE19 pKa = 4.44SICPEE24 pKa = 3.84VFEE27 pKa = 5.53VIDD30 pKa = 3.54ISQVKK35 pKa = 9.51EE36 pKa = 3.84GVVFADD42 pKa = 4.88FEE44 pKa = 4.74AGITDD49 pKa = 3.79AADD52 pKa = 3.51NCPVSVIKK60 pKa = 10.5FEE62 pKa = 4.12

Molecular weight: 6.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C2BYG0|A0A1C2BYG0_9PORP Choloylglycine hydrolase OS=Macellibacteroides sp. HH-ZS OX=1857568 GN=A9168_03900 PE=3 SV=1
MM1 pKa = 7.87IITIKK6 pKa = 10.3IRR8 pKa = 11.84MKK10 pKa = 8.97NTEE13 pKa = 4.42EE14 pKa = 4.35IPVKK18 pKa = 10.45SYY20 pKa = 11.4SLSEE24 pKa = 4.0LSQLFSPSLSLSASTKK40 pKa = 10.25QLLRR44 pKa = 11.84WMLRR48 pKa = 11.84HH49 pKa = 6.25PNLVLRR55 pKa = 11.84LQEE58 pKa = 4.16TRR60 pKa = 11.84WQKK63 pKa = 9.85GGRR66 pKa = 11.84RR67 pKa = 11.84FSPKK71 pKa = 10.02QISLLFDD78 pKa = 4.88CIGPPP83 pKa = 3.62

Molecular weight: 9.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3239

0

3239

1208148

56

2479

373.0

41.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.991 ± 0.035

1.089 ± 0.016

5.414 ± 0.027

6.327 ± 0.036

4.773 ± 0.026

6.781 ± 0.041

1.725 ± 0.018

7.118 ± 0.042

6.888 ± 0.036

9.351 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.018

5.315 ± 0.036

3.779 ± 0.021

3.413 ± 0.024

4.273 ± 0.026

6.574 ± 0.032

5.523 ± 0.027

6.534 ± 0.031

1.221 ± 0.017

4.294 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski