Mikumi yellow baboon virus 1
Average proteome isoelectric point is 7.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A089G1Z7|A0A089G1Z7_9NIDO GP4 protein OS=Mikumi yellow baboon virus 1 OX=1546177 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.68 APLIHH6 pKa = 6.72 GRR8 pKa = 11.84 FISCFCVLYY17 pKa = 8.1 FVCSCCANNNTTGTTPTGVCFLLPMKK43 pKa = 10.68 HH44 pKa = 6.49 NITINITLSTLFCTNDD60 pKa = 3.05 GKK62 pKa = 11.34 VEE64 pKa = 3.96 MEE66 pKa = 3.32 VDD68 pKa = 3.62 EE69 pKa = 4.56 NHH71 pKa = 6.83 FGDD74 pKa = 5.34 DD75 pKa = 3.82 EE76 pKa = 5.03 CPLSGFKK83 pKa = 10.3 PHH85 pKa = 6.91 GSTKK89 pKa = 10.3 GKK91 pKa = 8.87 YY92 pKa = 10.08 GSFIHH97 pKa = 6.97 HH98 pKa = 7.51 LDD100 pKa = 3.06 QSFPLNLSSNPSHH113 pKa = 7.47 VYY115 pKa = 8.41 ITILLTYY122 pKa = 9.94 IVAQYY127 pKa = 10.71 PSVLFDD133 pKa = 3.02 NTTNRR138 pKa = 11.84 VYY140 pKa = 10.82 AINATEE146 pKa = 3.97 TAGEE150 pKa = 3.85 WRR152 pKa = 11.84 FCVNATDD159 pKa = 5.25 LAALNQTLPIVDD171 pKa = 5.67 LYY173 pKa = 9.01 TTGPPWDD180 pKa = 3.87 LYY182 pKa = 10.51 YY183 pKa = 10.95 VEE185 pKa = 5.02 LLRR188 pKa = 11.84 PFLLSLLMLGLSRR201 pKa = 11.84 II202 pKa = 4.1
Molecular weight: 22.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.403
IPC2_protein 5.308
IPC_protein 5.283
Toseland 5.563
ProMoST 5.588
Dawson 5.486
Bjellqvist 5.525
Wikipedia 5.474
Rodwell 5.448
Grimsley 5.652
Solomon 5.486
Lehninger 5.474
Nozaki 5.728
DTASelect 5.919
Thurlkill 5.855
EMBOSS 5.83
Sillero 5.817
Patrickios 0.413
IPC_peptide 5.499
IPC2_peptide 5.83
IPC2.peptide.svr19 5.766
Protein with the highest isoelectric point:
>tr|A0A089G0E4|A0A089G0E4_9NIDO ORF2a' protein OS=Mikumi yellow baboon virus 1 OX=1546177 GN=ORF2a' PE=4 SV=1
MM1 pKa = 7.3 VSAICADD8 pKa = 3.63 PGYY11 pKa = 6.9 TTLAFTAAPVFIACLRR27 pKa = 11.84 LFTPNIRR34 pKa = 11.84 GLACLIIVSTLAYY47 pKa = 10.04 AATKK51 pKa = 9.83 FAEE54 pKa = 4.46 HH55 pKa = 6.46 SLATVVTIAFSLLYY69 pKa = 10.5 CSFKK73 pKa = 10.48 FVQWLIIRR81 pKa = 11.84 IRR83 pKa = 11.84 MCRR86 pKa = 11.84 LGRR89 pKa = 11.84 QYY91 pKa = 10.59 IISPASHH98 pKa = 6.46 VEE100 pKa = 4.1 TSFGRR105 pKa = 11.84 YY106 pKa = 8.71 SLPSTGSSAVVTRR119 pKa = 11.84 RR120 pKa = 11.84 SGMTLVNNQLIPDD133 pKa = 3.91 VKK135 pKa = 10.8 KK136 pKa = 10.72 LVLAGKK142 pKa = 9.7 IATKK146 pKa = 10.29 KK147 pKa = 10.72 GLINLRR153 pKa = 11.84 KK154 pKa = 10.2 YY155 pKa = 10.28 GWQKK159 pKa = 9.55 TKK161 pKa = 11.02
Molecular weight: 17.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.736
IPC_protein 10.058
Toseland 10.394
ProMoST 10.087
Dawson 10.54
Bjellqvist 10.233
Wikipedia 10.716
Rodwell 10.979
Grimsley 10.613
Solomon 10.584
Lehninger 10.555
Nozaki 10.409
DTASelect 10.218
Thurlkill 10.409
EMBOSS 10.774
Sillero 10.467
Patrickios 10.701
IPC_peptide 10.584
IPC2_peptide 9.311
IPC2.peptide.svr19 8.501
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
7301
47
3539
561.6
61.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.903 ± 0.455
3.205 ± 0.235
4.383 ± 0.575
3.383 ± 0.339
4.534 ± 0.416
6.876 ± 0.751
3.013 ± 0.473
5.013 ± 0.388
4.109 ± 0.382
10.273 ± 0.774
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.548 ± 0.176
3.123 ± 0.527
6.41 ± 0.595
3.095 ± 0.157
4.684 ± 0.285
7.478 ± 0.4
7.246 ± 0.507
7.67 ± 0.293
1.082 ± 0.161
3.972 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here