Epizootic hemorrhagic disease virus (serotype 1 / strain New Jersey)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus; Epizootic hemorrhagic disease virus; Epizootic hemorrhagic disease virus 1

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8TE66|C8TE66_EHDV1 VP6 protein OS=Epizootic hemorrhagic disease virus (serotype 1 / strain New Jersey) OX=449133 GN=VP6 PE=4 SV=1
MM1 pKa = 7.04EE2 pKa = 4.1QKK4 pKa = 9.85QRR6 pKa = 11.84RR7 pKa = 11.84FTKK10 pKa = 10.4NVFVLDD16 pKa = 3.85QKK18 pKa = 11.27RR19 pKa = 11.84KK20 pKa = 6.48TLCGQIAARR29 pKa = 11.84QSLPYY34 pKa = 9.69CQIKK38 pKa = 9.85IGRR41 pKa = 11.84NFALKK46 pKa = 10.27AVATPEE52 pKa = 3.8PKK54 pKa = 10.48GYY56 pKa = 10.52VLEE59 pKa = 4.56ICDD62 pKa = 3.36VGAYY66 pKa = 10.01RR67 pKa = 11.84IQDD70 pKa = 3.68GNDD73 pKa = 3.35VISLMISADD82 pKa = 3.52GVEE85 pKa = 4.75GTQEE89 pKa = 3.66RR90 pKa = 11.84WEE92 pKa = 3.97EE93 pKa = 4.01WKK95 pKa = 10.86FEE97 pKa = 4.43TISCVPMATVLNINGALVDD116 pKa = 3.95AEE118 pKa = 4.3IKK120 pKa = 10.02VSKK123 pKa = 10.08GMGMVPPYY131 pKa = 9.61TRR133 pKa = 11.84NDD135 pKa = 3.09FDD137 pKa = 4.59RR138 pKa = 11.84RR139 pKa = 11.84EE140 pKa = 4.08LPEE143 pKa = 4.19LPGVQRR149 pKa = 11.84SKK151 pKa = 11.24YY152 pKa = 9.96DD153 pKa = 3.29IRR155 pKa = 11.84EE156 pKa = 3.83LRR158 pKa = 11.84QKK160 pKa = 10.21IRR162 pKa = 11.84EE163 pKa = 4.03EE164 pKa = 4.1RR165 pKa = 11.84EE166 pKa = 3.69KK167 pKa = 11.24GAVEE171 pKa = 4.21QPHH174 pKa = 6.38KK175 pKa = 10.35PAFKK179 pKa = 9.28TEE181 pKa = 3.91RR182 pKa = 11.84WHH184 pKa = 5.9EE185 pKa = 4.08QPDD188 pKa = 3.57SDD190 pKa = 4.94EE191 pKa = 4.56DD192 pKa = 3.54QNLAGGVVNDD202 pKa = 4.25WTCEE206 pKa = 3.94TQEE209 pKa = 4.01RR210 pKa = 11.84DD211 pKa = 3.12QEE213 pKa = 4.26AEE215 pKa = 3.69RR216 pKa = 11.84RR217 pKa = 11.84EE218 pKa = 3.97ALEE221 pKa = 3.68IRR223 pKa = 11.84LAEE226 pKa = 3.68QRR228 pKa = 11.84QRR230 pKa = 11.84IEE232 pKa = 3.79AFKK235 pKa = 10.79LDD237 pKa = 3.99SEE239 pKa = 4.63RR240 pKa = 11.84KK241 pKa = 9.28RR242 pKa = 11.84EE243 pKa = 3.92DD244 pKa = 3.2LEE246 pKa = 4.51RR247 pKa = 11.84SQEE250 pKa = 3.85QFRR253 pKa = 11.84TQEE256 pKa = 4.2VEE258 pKa = 3.72WKK260 pKa = 9.53KK261 pKa = 10.77PDD263 pKa = 3.18MGEE266 pKa = 4.2IIIEE270 pKa = 4.12EE271 pKa = 4.2DD272 pKa = 3.78DD273 pKa = 3.66EE274 pKa = 5.99DD275 pKa = 4.86SEE277 pKa = 4.56EE278 pKa = 4.33EE279 pKa = 3.82EE280 pKa = 4.55GARR283 pKa = 11.84ASYY286 pKa = 8.49ITSAYY291 pKa = 8.91IEE293 pKa = 4.62RR294 pKa = 11.84ISRR297 pKa = 11.84IRR299 pKa = 11.84KK300 pKa = 9.21IKK302 pKa = 10.19DD303 pKa = 2.93EE304 pKa = 4.35RR305 pKa = 11.84LSMLASMMPQQSGEE319 pKa = 4.06YY320 pKa = 6.62TTTLFIKK327 pKa = 9.15KK328 pKa = 9.36QKK330 pKa = 8.54WDD332 pKa = 3.81NVPLYY337 pKa = 10.77LIDD340 pKa = 4.24EE341 pKa = 4.34MQKK344 pKa = 10.31KK345 pKa = 9.72YY346 pKa = 10.64EE347 pKa = 4.01LQSVGSCEE355 pKa = 3.58RR356 pKa = 11.84VAFVSKK362 pKa = 9.14GTNLIILPVGVV373 pKa = 3.86

Molecular weight: 43.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C9QNK7|C9QNK7_EHDV1 Non-structural protein NS3 OS=Epizootic hemorrhagic disease virus (serotype 1 / strain New Jersey) OX=449133 GN=NS3 PE=3 SV=1
MM1 pKa = 7.88LSRR4 pKa = 11.84LVPGVEE10 pKa = 4.01TRR12 pKa = 11.84IEE14 pKa = 4.02MKK16 pKa = 10.77ASDD19 pKa = 4.27EE20 pKa = 4.26VSLVPYY26 pKa = 9.71QEE28 pKa = 4.0QVRR31 pKa = 11.84PPSYY35 pKa = 10.68VPSAPIPTAMPRR47 pKa = 11.84VALDD51 pKa = 3.46ILDD54 pKa = 3.97KK55 pKa = 11.5AMSNQTGATMAQKK68 pKa = 10.38VEE70 pKa = 4.11KK71 pKa = 10.19VAYY74 pKa = 9.76ASYY77 pKa = 11.54AEE79 pKa = 4.37AFRR82 pKa = 11.84DD83 pKa = 3.96DD84 pKa = 3.69LRR86 pKa = 11.84LRR88 pKa = 11.84QIKK91 pKa = 9.97KK92 pKa = 9.74HH93 pKa = 5.3VNEE96 pKa = 4.13QILPKK101 pKa = 9.92MRR103 pKa = 11.84VEE105 pKa = 4.12LTAMKK110 pKa = 10.09RR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84AMAHH117 pKa = 5.06MVLIIAAVVALITSASTLTSDD138 pKa = 4.25LGVILKK144 pKa = 10.67HH145 pKa = 5.3NTTTEE150 pKa = 4.75AIQTYY155 pKa = 10.06IKK157 pKa = 9.49PFCAAFGIINLAATMVMMFMAKK179 pKa = 9.51NEE181 pKa = 4.26KK182 pKa = 10.04IINQQIDD189 pKa = 3.54HH190 pKa = 5.65TRR192 pKa = 11.84KK193 pKa = 10.24EE194 pKa = 4.06IMKK197 pKa = 9.93KK198 pKa = 10.03DD199 pKa = 3.6AYY201 pKa = 10.75NEE203 pKa = 3.94AVRR206 pKa = 11.84MSVTEE211 pKa = 3.98FSGIPLDD218 pKa = 4.54GFDD221 pKa = 5.09IPPEE225 pKa = 4.01LTRR228 pKa = 5.13

Molecular weight: 25.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6204

228

1302

620.4

71.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.399 ± 0.556

1.177 ± 0.242

6.044 ± 0.364

7.963 ± 0.612

3.82 ± 0.302

5.77 ± 0.339

2.144 ± 0.234

7.447 ± 0.488

6.254 ± 0.684

8.333 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.594 ± 0.422

3.965 ± 0.387

3.949 ± 0.305

4.094 ± 0.283

7.205 ± 0.385

5.255 ± 0.353

4.932 ± 0.322

6.593 ± 0.331

1.209 ± 0.242

3.852 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski