Beihai narna-like virus 16
Average proteome isoelectric point is 5.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KI67|A0A1L3KI67_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 16 OX=1922443 PE=4 SV=1
MM1 pKa = 7.68 DD2 pKa = 5.43 HH3 pKa = 6.72 SLNYY7 pKa = 9.71 YY8 pKa = 9.72 RR9 pKa = 11.84 RR10 pKa = 11.84 STIFRR15 pKa = 11.84 KK16 pKa = 9.63 EE17 pKa = 3.9 VGPQIYY23 pKa = 10.48 QNDD26 pKa = 3.93 FWEE29 pKa = 4.91 VIQDD33 pKa = 3.64 KK34 pKa = 10.97 QLLQNINLVFKK45 pKa = 10.52 EE46 pKa = 3.72 RR47 pKa = 11.84 TKK49 pKa = 9.68 FHH51 pKa = 6.11 EE52 pKa = 4.39 NIRR55 pKa = 11.84 RR56 pKa = 11.84 KK57 pKa = 9.18 WIQKK61 pKa = 8.93 IKK63 pKa = 10.53 SHH65 pKa = 6.15 VEE67 pKa = 3.84 TPEE70 pKa = 3.85 TQIGKK75 pKa = 9.25 EE76 pKa = 4.33 FYY78 pKa = 10.41 ASEE81 pKa = 4.07 IAPFLPTSLGFDD93 pKa = 3.84 ASPEE97 pKa = 3.95 IRR99 pKa = 11.84 SQEE102 pKa = 3.83 YY103 pKa = 8.7 TLEE106 pKa = 4.08 FDD108 pKa = 4.03 AFPPEE113 pKa = 3.59 EE114 pKa = 4.21 SLPVRR119 pKa = 11.84 VEE121 pKa = 3.83 PVIEE125 pKa = 3.97 PLKK128 pKa = 10.75 VRR130 pKa = 11.84 TITAGRR136 pKa = 11.84 GDD138 pKa = 4.64 CFCLKK143 pKa = 10.11 PFQRR147 pKa = 11.84 AMWLALGEE155 pKa = 4.2 EE156 pKa = 4.36 KK157 pKa = 10.59 QFCLTHH163 pKa = 5.47 GTNNLEE169 pKa = 4.0 PAIKK173 pKa = 9.89 RR174 pKa = 11.84 IYY176 pKa = 9.96 EE177 pKa = 3.98 KK178 pKa = 10.79 SDD180 pKa = 3.6 PNDD183 pKa = 2.73 VWISGDD189 pKa = 3.36 YY190 pKa = 9.18 TAATDD195 pKa = 4.63 CIPIEE200 pKa = 4.09 VSKK203 pKa = 9.7 TLLEE207 pKa = 5.19 GILEE211 pKa = 4.8 SIDD214 pKa = 3.76 HH215 pKa = 6.67 EE216 pKa = 4.39 PTKK219 pKa = 10.37 RR220 pKa = 11.84 WAMKK224 pKa = 9.97 EE225 pKa = 3.86 ISPHH229 pKa = 5.85 LLVYY233 pKa = 10.35 PKK235 pKa = 10.59 DD236 pKa = 3.8 SGIEE240 pKa = 3.84 PVLQEE245 pKa = 4.21 SGQLMGSLLSFPLLCLLNDD264 pKa = 3.86 CTAQSVGLSPEE275 pKa = 4.08 KK276 pKa = 10.91 YY277 pKa = 10.25 LINGDD282 pKa = 4.63 DD283 pKa = 3.44 ILMRR287 pKa = 11.84 APQKK291 pKa = 10.95 VYY293 pKa = 11.02 SSWKK297 pKa = 9.68 KK298 pKa = 9.65 NVNSFGLSLSLGKK311 pKa = 10.42 NYY313 pKa = 9.77 IHH315 pKa = 7.69 PDD317 pKa = 3.23 FGTVNSQLILRR328 pKa = 11.84 GDD330 pKa = 3.77 VLCSGKK336 pKa = 10.25 QKK338 pKa = 10.86 VLDD341 pKa = 3.58 RR342 pKa = 11.84 RR343 pKa = 11.84 SRR345 pKa = 11.84 ILGEE349 pKa = 4.06 CLRR352 pKa = 11.84 DD353 pKa = 3.66 LEE355 pKa = 4.7 VMMRR359 pKa = 11.84 DD360 pKa = 3.64 TSPDD364 pKa = 3.37 DD365 pKa = 3.53 VKK367 pKa = 11.45 EE368 pKa = 3.9 LFKK371 pKa = 10.67 TVNRR375 pKa = 11.84 SKK377 pKa = 10.83 LSKK380 pKa = 8.61 TVRR383 pKa = 11.84 DD384 pKa = 3.39 ISIPMSHH391 pKa = 6.74 GGLALEE397 pKa = 4.63 WGSSKK402 pKa = 10.57 KK403 pKa = 10.24 DD404 pKa = 3.23 EE405 pKa = 4.02 RR406 pKa = 11.84 TQRR409 pKa = 11.84 TNILVYY415 pKa = 10.15 FHH417 pKa = 7.43 DD418 pKa = 5.03 LFRR421 pKa = 11.84 KK422 pKa = 9.64 IEE424 pKa = 3.95 PHH426 pKa = 6.07 SGCLAIPYY434 pKa = 9.44 LSNSVFHH441 pKa = 5.8 QSQVEE446 pKa = 4.19 EE447 pKa = 3.92 MDD449 pKa = 3.38 KK450 pKa = 10.69 MFNEE454 pKa = 5.28 PILSKK459 pKa = 10.29 EE460 pKa = 3.79 YY461 pKa = 9.91 HH462 pKa = 6.39 EE463 pKa = 4.63 EE464 pKa = 4.26 FIGIPQLNIVRR475 pKa = 11.84 KK476 pKa = 9.74 RR477 pKa = 11.84 FMSHH481 pKa = 4.67 SQLRR485 pKa = 11.84 DD486 pKa = 3.16 LFLGQDD492 pKa = 3.45 IQDD495 pKa = 4.13 LPSLTFLKK503 pKa = 10.52 VIQVPFNDD511 pKa = 3.34 GKK513 pKa = 10.4 IRR515 pKa = 11.84 KK516 pKa = 8.32 EE517 pKa = 3.58 IQKK520 pKa = 10.37 EE521 pKa = 3.2 IDD523 pKa = 3.67 RR524 pKa = 11.84 IFFLNFLDD532 pKa = 3.94 SNRR535 pKa = 11.84 EE536 pKa = 3.69 YY537 pKa = 10.77 GYY539 pKa = 10.72 EE540 pKa = 3.82 AFRR543 pKa = 11.84 SSFLEE548 pKa = 3.79 AVRR551 pKa = 11.84 GIPSATEE558 pKa = 3.64 VATKK562 pKa = 10.21 FLTPIIEE569 pKa = 4.41 LDD571 pKa = 3.48 VRR573 pKa = 11.84 PDD575 pKa = 3.41 YY576 pKa = 10.85 LLKK579 pKa = 10.62 VVKK582 pKa = 9.95 DD583 pKa = 3.72 YY584 pKa = 11.46 KK585 pKa = 9.95 VTNFDD590 pKa = 3.51 GEE592 pKa = 4.45 LFEE595 pKa = 4.66 KK596 pKa = 10.79 QLGKK600 pKa = 10.53 ALQPKK605 pKa = 9.4 NFNLPFDD612 pKa = 4.58 LEE614 pKa = 4.45 SPDD617 pKa = 3.5 FSKK620 pKa = 11.08 EE621 pKa = 4.04 VVSSFDD627 pKa = 3.48 EE628 pKa = 4.22 VLGSLDD634 pKa = 3.33 QTEE637 pKa = 4.41 KK638 pKa = 11.34 DD639 pKa = 3.87 FFGLPTADD647 pKa = 3.2 ADD649 pKa = 4.46 RR650 pKa = 11.84 FNKK653 pKa = 9.74 SFWYY657 pKa = 10.04 FPEE660 pKa = 4.14 EE661 pKa = 3.79
Molecular weight: 76.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.416
IPC2_protein 5.423
IPC_protein 5.423
Toseland 5.55
ProMoST 5.575
Dawson 5.512
Bjellqvist 5.601
Wikipedia 5.423
Rodwell 5.448
Grimsley 5.563
Solomon 5.512
Lehninger 5.474
Nozaki 5.69
DTASelect 5.842
Thurlkill 5.652
EMBOSS 5.601
Sillero 5.766
Patrickios 4.329
IPC_peptide 5.525
IPC2_peptide 5.766
IPC2.peptide.svr19 5.766
Protein with the highest isoelectric point:
>tr|A0A1L3KI67|A0A1L3KI67_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 16 OX=1922443 PE=4 SV=1
MM1 pKa = 7.68 DD2 pKa = 5.43 HH3 pKa = 6.72 SLNYY7 pKa = 9.71 YY8 pKa = 9.72 RR9 pKa = 11.84 RR10 pKa = 11.84 STIFRR15 pKa = 11.84 KK16 pKa = 9.63 EE17 pKa = 3.9 VGPQIYY23 pKa = 10.48 QNDD26 pKa = 3.93 FWEE29 pKa = 4.91 VIQDD33 pKa = 3.64 KK34 pKa = 10.97 QLLQNINLVFKK45 pKa = 10.52 EE46 pKa = 3.72 RR47 pKa = 11.84 TKK49 pKa = 9.68 FHH51 pKa = 6.11 EE52 pKa = 4.39 NIRR55 pKa = 11.84 RR56 pKa = 11.84 KK57 pKa = 9.18 WIQKK61 pKa = 8.93 IKK63 pKa = 10.53 SHH65 pKa = 6.15 VEE67 pKa = 3.84 TPEE70 pKa = 3.85 TQIGKK75 pKa = 9.25 EE76 pKa = 4.33 FYY78 pKa = 10.41 ASEE81 pKa = 4.07 IAPFLPTSLGFDD93 pKa = 3.84 ASPEE97 pKa = 3.95 IRR99 pKa = 11.84 SQEE102 pKa = 3.83 YY103 pKa = 8.7 TLEE106 pKa = 4.08 FDD108 pKa = 4.03 AFPPEE113 pKa = 3.59 EE114 pKa = 4.21 SLPVRR119 pKa = 11.84 VEE121 pKa = 3.83 PVIEE125 pKa = 3.97 PLKK128 pKa = 10.75 VRR130 pKa = 11.84 TITAGRR136 pKa = 11.84 GDD138 pKa = 4.64 CFCLKK143 pKa = 10.11 PFQRR147 pKa = 11.84 AMWLALGEE155 pKa = 4.2 EE156 pKa = 4.36 KK157 pKa = 10.59 QFCLTHH163 pKa = 5.47 GTNNLEE169 pKa = 4.0 PAIKK173 pKa = 9.89 RR174 pKa = 11.84 IYY176 pKa = 9.96 EE177 pKa = 3.98 KK178 pKa = 10.79 SDD180 pKa = 3.6 PNDD183 pKa = 2.73 VWISGDD189 pKa = 3.36 YY190 pKa = 9.18 TAATDD195 pKa = 4.63 CIPIEE200 pKa = 4.09 VSKK203 pKa = 9.7 TLLEE207 pKa = 5.19 GILEE211 pKa = 4.8 SIDD214 pKa = 3.76 HH215 pKa = 6.67 EE216 pKa = 4.39 PTKK219 pKa = 10.37 RR220 pKa = 11.84 WAMKK224 pKa = 9.97 EE225 pKa = 3.86 ISPHH229 pKa = 5.85 LLVYY233 pKa = 10.35 PKK235 pKa = 10.59 DD236 pKa = 3.8 SGIEE240 pKa = 3.84 PVLQEE245 pKa = 4.21 SGQLMGSLLSFPLLCLLNDD264 pKa = 3.86 CTAQSVGLSPEE275 pKa = 4.08 KK276 pKa = 10.91 YY277 pKa = 10.25 LINGDD282 pKa = 4.63 DD283 pKa = 3.44 ILMRR287 pKa = 11.84 APQKK291 pKa = 10.95 VYY293 pKa = 11.02 SSWKK297 pKa = 9.68 KK298 pKa = 9.65 NVNSFGLSLSLGKK311 pKa = 10.42 NYY313 pKa = 9.77 IHH315 pKa = 7.69 PDD317 pKa = 3.23 FGTVNSQLILRR328 pKa = 11.84 GDD330 pKa = 3.77 VLCSGKK336 pKa = 10.25 QKK338 pKa = 10.86 VLDD341 pKa = 3.58 RR342 pKa = 11.84 RR343 pKa = 11.84 SRR345 pKa = 11.84 ILGEE349 pKa = 4.06 CLRR352 pKa = 11.84 DD353 pKa = 3.66 LEE355 pKa = 4.7 VMMRR359 pKa = 11.84 DD360 pKa = 3.64 TSPDD364 pKa = 3.37 DD365 pKa = 3.53 VKK367 pKa = 11.45 EE368 pKa = 3.9 LFKK371 pKa = 10.67 TVNRR375 pKa = 11.84 SKK377 pKa = 10.83 LSKK380 pKa = 8.61 TVRR383 pKa = 11.84 DD384 pKa = 3.39 ISIPMSHH391 pKa = 6.74 GGLALEE397 pKa = 4.63 WGSSKK402 pKa = 10.57 KK403 pKa = 10.24 DD404 pKa = 3.23 EE405 pKa = 4.02 RR406 pKa = 11.84 TQRR409 pKa = 11.84 TNILVYY415 pKa = 10.15 FHH417 pKa = 7.43 DD418 pKa = 5.03 LFRR421 pKa = 11.84 KK422 pKa = 9.64 IEE424 pKa = 3.95 PHH426 pKa = 6.07 SGCLAIPYY434 pKa = 9.44 LSNSVFHH441 pKa = 5.8 QSQVEE446 pKa = 4.19 EE447 pKa = 3.92 MDD449 pKa = 3.38 KK450 pKa = 10.69 MFNEE454 pKa = 5.28 PILSKK459 pKa = 10.29 EE460 pKa = 3.79 YY461 pKa = 9.91 HH462 pKa = 6.39 EE463 pKa = 4.63 EE464 pKa = 4.26 FIGIPQLNIVRR475 pKa = 11.84 KK476 pKa = 9.74 RR477 pKa = 11.84 FMSHH481 pKa = 4.67 SQLRR485 pKa = 11.84 DD486 pKa = 3.16 LFLGQDD492 pKa = 3.45 IQDD495 pKa = 4.13 LPSLTFLKK503 pKa = 10.52 VIQVPFNDD511 pKa = 3.34 GKK513 pKa = 10.4 IRR515 pKa = 11.84 KK516 pKa = 8.32 EE517 pKa = 3.58 IQKK520 pKa = 10.37 EE521 pKa = 3.2 IDD523 pKa = 3.67 RR524 pKa = 11.84 IFFLNFLDD532 pKa = 3.94 SNRR535 pKa = 11.84 EE536 pKa = 3.69 YY537 pKa = 10.77 GYY539 pKa = 10.72 EE540 pKa = 3.82 AFRR543 pKa = 11.84 SSFLEE548 pKa = 3.79 AVRR551 pKa = 11.84 GIPSATEE558 pKa = 3.64 VATKK562 pKa = 10.21 FLTPIIEE569 pKa = 4.41 LDD571 pKa = 3.48 VRR573 pKa = 11.84 PDD575 pKa = 3.41 YY576 pKa = 10.85 LLKK579 pKa = 10.62 VVKK582 pKa = 9.95 DD583 pKa = 3.72 YY584 pKa = 11.46 KK585 pKa = 9.95 VTNFDD590 pKa = 3.51 GEE592 pKa = 4.45 LFEE595 pKa = 4.66 KK596 pKa = 10.79 QLGKK600 pKa = 10.53 ALQPKK605 pKa = 9.4 NFNLPFDD612 pKa = 4.58 LEE614 pKa = 4.45 SPDD617 pKa = 3.5 FSKK620 pKa = 11.08 EE621 pKa = 4.04 VVSSFDD627 pKa = 3.48 EE628 pKa = 4.22 VLGSLDD634 pKa = 3.33 QTEE637 pKa = 4.41 KK638 pKa = 11.34 DD639 pKa = 3.87 FFGLPTADD647 pKa = 3.2 ADD649 pKa = 4.46 RR650 pKa = 11.84 FNKK653 pKa = 9.74 SFWYY657 pKa = 10.04 FPEE660 pKa = 4.14 EE661 pKa = 3.79
Molecular weight: 76.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.416
IPC2_protein 5.423
IPC_protein 5.423
Toseland 5.55
ProMoST 5.575
Dawson 5.512
Bjellqvist 5.601
Wikipedia 5.423
Rodwell 5.448
Grimsley 5.563
Solomon 5.512
Lehninger 5.474
Nozaki 5.69
DTASelect 5.842
Thurlkill 5.652
EMBOSS 5.601
Sillero 5.766
Patrickios 4.329
IPC_peptide 5.525
IPC2_peptide 5.766
IPC2.peptide.svr19 5.766
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
661
661
661
661.0
76.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.328 ± 0.0
1.362 ± 0.0
6.505 ± 0.0
8.169 ± 0.0
6.354 ± 0.0
4.992 ± 0.0
1.967 ± 0.0
6.657 ± 0.0
7.262 ± 0.0
10.741 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.664 ± 0.0
3.933 ± 0.0
5.749 ± 0.0
4.236 ± 0.0
5.295 ± 0.0
7.867 ± 0.0
4.236 ± 0.0
5.598 ± 0.0
1.21 ± 0.0
2.874 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here