Tenacibaculum phage pT24
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 297 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4XWQ7|A0A1B4XWQ7_9CAUD Uncharacterized protein OS=Tenacibaculum phage pT24 OX=1880590 GN=BPT24_100 PE=4 SV=1
MM1 pKa = 7.07 IKK3 pKa = 9.96 EE4 pKa = 4.16 RR5 pKa = 11.84 KK6 pKa = 8.79 FDD8 pKa = 3.66 QNGEE12 pKa = 4.18 YY13 pKa = 9.92 PIEE16 pKa = 4.16 EE17 pKa = 4.68 SNFEE21 pKa = 4.33 LFSGKK26 pKa = 10.43 DD27 pKa = 3.28 NVYY30 pKa = 11.0 SSIVEE35 pKa = 4.04 IKK37 pKa = 9.71 TKK39 pKa = 10.66 DD40 pKa = 3.67 GNKK43 pKa = 9.92 HH44 pKa = 4.45 YY45 pKa = 9.63 ATYY48 pKa = 10.91 NITEE52 pKa = 4.63 DD53 pKa = 3.71 VFKK56 pKa = 10.97 LIPNINDD63 pKa = 3.35 DD64 pKa = 4.08 SVEE67 pKa = 4.17 HH68 pKa = 5.98 GVEE71 pKa = 4.14 GTGDD75 pKa = 3.78 NLPSEE80 pKa = 5.1 NVEE83 pKa = 3.56 TWKK86 pKa = 10.38 PYY88 pKa = 10.66 LCLTTLVDD96 pKa = 4.28 EE97 pKa = 4.89 LQGTCKK103 pKa = 10.66 SLDD106 pKa = 3.75 EE107 pKa = 4.56 ACEE110 pKa = 4.09 TQHH113 pKa = 6.89 IDD115 pKa = 4.4 CFEE118 pKa = 4.75 LNIDD122 pKa = 3.95 EE123 pKa = 5.3 LGKK126 pKa = 10.04 IDD128 pKa = 5.57 NEE130 pKa = 4.02 IFNCAEE136 pKa = 4.23 CGWWCEE142 pKa = 3.76 ISEE145 pKa = 4.08 QAEE148 pKa = 4.26 DD149 pKa = 4.68 GDD151 pKa = 5.14 DD152 pKa = 4.14 SVCVDD157 pKa = 3.65 CCEE160 pKa = 3.83
Molecular weight: 18.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.812
IPC2_protein 3.973
IPC_protein 3.935
Toseland 3.745
ProMoST 4.037
Dawson 3.897
Bjellqvist 4.075
Wikipedia 3.783
Rodwell 3.757
Grimsley 3.656
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.164
Thurlkill 3.77
EMBOSS 3.795
Sillero 4.05
Patrickios 0.693
IPC_peptide 3.884
IPC2_peptide 4.024
IPC2.peptide.svr19 3.955
Protein with the highest isoelectric point:
>tr|A0A1W7GKP2|A0A1W7GKP2_9CAUD Site-specific DNA-methyltransferase (adenine-specific) OS=Tenacibaculum phage pT24 OX=1880590 GN=BPT24_034 PE=4 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.12 IKK4 pKa = 10.44 SLKK7 pKa = 10.15 DD8 pKa = 3.1 PKK10 pKa = 10.38 FRR12 pKa = 11.84 QTWGSHH18 pKa = 4.57 SNTSRR23 pKa = 11.84 RR24 pKa = 11.84 ALNKK28 pKa = 9.96 KK29 pKa = 8.27 FTMCCGVRR37 pKa = 11.84 NCNYY41 pKa = 9.79 SSRR44 pKa = 11.84 AYY46 pKa = 10.48 RR47 pKa = 11.84 ITDD50 pKa = 3.63 SQSKK54 pKa = 5.78 THH56 pKa = 6.24 NSSYY60 pKa = 10.58 RR61 pKa = 11.84 CPKK64 pKa = 10.52 HH65 pKa = 6.61 GDD67 pKa = 3.31 TLILIGDD74 pKa = 4.35 VMQLPRR80 pKa = 11.84 NRR82 pKa = 11.84 RR83 pKa = 11.84 KK84 pKa = 10.28 RR85 pKa = 11.84 NLLIKK90 pKa = 10.35 KK91 pKa = 9.03 FRR93 pKa = 3.85
Molecular weight: 10.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.282
IPC2_protein 10.014
IPC_protein 10.979
Toseland 11.213
ProMoST 11.403
Dawson 11.257
Bjellqvist 11.038
Wikipedia 11.535
Rodwell 11.477
Grimsley 11.286
Solomon 11.506
Lehninger 11.447
Nozaki 11.199
DTASelect 11.038
Thurlkill 11.199
EMBOSS 11.637
Sillero 11.213
Patrickios 11.199
IPC_peptide 11.52
IPC2_peptide 10.321
IPC2.peptide.svr19 8.656
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
297
0
297
72176
26
3229
243.0
27.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.665 ± 0.165
0.921 ± 0.064
6.911 ± 0.139
7.5 ± 0.212
5.115 ± 0.109
5.326 ± 0.326
1.642 ± 0.052
8.129 ± 0.118
9.227 ± 0.359
8.402 ± 0.145
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.584 ± 0.114
7.364 ± 0.206
2.38 ± 0.073
2.688 ± 0.065
3.584 ± 0.111
7.192 ± 0.173
5.757 ± 0.223
6.03 ± 0.117
0.844 ± 0.035
4.74 ± 0.111
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here