Aliarcobacter faecis
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 124 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M8N3P8|A0A6M8N3P8_9PROT Uncharacterized protein OS=Aliarcobacter faecis OX=1564138 GN=AFAEC_a0121 PE=4 SV=1
MM1 pKa = 7.09 QVQNEE6 pKa = 4.4 KK7 pKa = 10.77 EE8 pKa = 4.29 LIQIPIDD15 pKa = 3.18 IFYY18 pKa = 11.03 DD19 pKa = 3.36 EE20 pKa = 4.34 FTVYY24 pKa = 10.46 EE25 pKa = 4.27 SNEE28 pKa = 3.63 RR29 pKa = 11.84 VAFEE33 pKa = 4.09 NFSEE37 pKa = 4.12 NKK39 pKa = 9.93 RR40 pKa = 11.84 YY41 pKa = 9.93 IIEE44 pKa = 4.18 YY45 pKa = 9.46 QDD47 pKa = 3.13 NDD49 pKa = 2.98 NVYY52 pKa = 9.79 VYY54 pKa = 10.43 KK55 pKa = 9.45 PTMQDD60 pKa = 3.48 LIKK63 pKa = 10.71 IMEE66 pKa = 4.68 GNFKK70 pKa = 10.83
Molecular weight: 8.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.321
IPC2_protein 4.38
IPC_protein 4.228
Toseland 4.075
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.05
Rodwell 4.062
Grimsley 3.986
Solomon 4.164
Lehninger 4.113
Nozaki 4.291
DTASelect 4.406
Thurlkill 4.088
EMBOSS 4.062
Sillero 4.329
Patrickios 3.389
IPC_peptide 4.164
IPC2_peptide 4.329
IPC2.peptide.svr19 4.247
Protein with the highest isoelectric point:
>tr|A0A6M8NBF4|A0A6M8NBF4_9PROT Uncharacterized protein OS=Aliarcobacter faecis OX=1564138 GN=AFAEC_a0087 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.3 KK3 pKa = 10.27 YY4 pKa = 10.9 LNQLKK9 pKa = 9.98 VAEE12 pKa = 4.24 TSGTKK17 pKa = 9.62 IGLFRR22 pKa = 11.84 TICSIFGGLMVAYY35 pKa = 10.39 LGMTLLVFIIPGTAGEE51 pKa = 4.44 SIIVSLLLNTLTWAIIALWISLSKK75 pKa = 9.66 TKK77 pKa = 10.8 YY78 pKa = 9.33 IALLRR83 pKa = 11.84 CIVPTLIFGILLIILYY99 pKa = 10.08 NII101 pKa = 4.1
Molecular weight: 11.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.179
IPC2_protein 9.209
IPC_protein 9.18
Toseland 9.692
ProMoST 9.472
Dawson 9.97
Bjellqvist 9.692
Wikipedia 10.145
Rodwell 10.306
Grimsley 10.043
Solomon 10.014
Lehninger 9.984
Nozaki 9.794
DTASelect 9.663
Thurlkill 9.794
EMBOSS 10.116
Sillero 9.897
Patrickios 7.468
IPC_peptide 10.014
IPC2_peptide 8.536
IPC2.peptide.svr19 8.088
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
124
0
124
37749
42
1307
304.4
35.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.921 ± 0.161
0.89 ± 0.067
5.799 ± 0.169
7.772 ± 0.225
5.547 ± 0.161
3.812 ± 0.188
1.69 ± 0.085
10.329 ± 0.195
10.509 ± 0.249
10.064 ± 0.245
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.796 ± 0.08
7.741 ± 0.208
2.331 ± 0.118
3.248 ± 0.145
3.001 ± 0.137
6.845 ± 0.201
4.782 ± 0.123
4.329 ± 0.13
0.763 ± 0.065
4.832 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here