Escherichia phage RB49 (Bacteriophage RB49)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Krischvirus; Escherichia virus RB49

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 277 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7Y3I8|Q7Y3I8_BPRB4 Uncharacterized protein OS=Escherichia phage RB49 OX=50948 GN=RB49ORF231c PE=4 SV=1
MM1 pKa = 7.54TKK3 pKa = 10.06LKK5 pKa = 10.43CVAVDD10 pKa = 4.67FEE12 pKa = 4.61LADD15 pKa = 3.71SFVVGDD21 pKa = 4.37EE22 pKa = 3.65YY23 pKa = 11.43DD24 pKa = 3.34ARR26 pKa = 11.84HH27 pKa = 6.96DD28 pKa = 3.77GTCYY32 pKa = 10.18YY33 pKa = 10.78VEE35 pKa = 4.7DD36 pKa = 4.37EE37 pKa = 5.17YY38 pKa = 11.75GVEE41 pKa = 4.0WAILPDD47 pKa = 4.02DD48 pKa = 3.7KK49 pKa = 11.27CFEE52 pKa = 4.44VVGG55 pKa = 4.07

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9G060|Q9G060_BPRB4 Co-chaperonin for GroEL OS=Escherichia phage RB49 OX=50948 GN=31 PE=4 SV=1
MM1 pKa = 7.79AIKK4 pKa = 10.31AGWVGSSAVSEE15 pKa = 4.32TGQRR19 pKa = 11.84WMSAAMQAVRR29 pKa = 11.84LGRR32 pKa = 11.84PAYY35 pKa = 8.54MSAMVGRR42 pKa = 11.84SKK44 pKa = 10.44EE45 pKa = 3.56IHH47 pKa = 5.97YY48 pKa = 10.81SIGASNSYY56 pKa = 11.1NKK58 pKa = 9.07DD59 pKa = 3.16TLINWMKK66 pKa = 10.69AQGSTPVVITITGNIVSQSTGVPCLDD92 pKa = 4.12FPSSLTNEE100 pKa = 4.16YY101 pKa = 8.69VTLIINSGVTVYY113 pKa = 10.98GRR115 pKa = 11.84GGNGGSNAAGAAGGNAINNAIGTRR139 pKa = 11.84LRR141 pKa = 11.84ITNNGAIAGGGGGGGGGNRR160 pKa = 11.84GRR162 pKa = 11.84LVFGGGGGRR171 pKa = 11.84PFGAGGSSSNMGSGATAGSISAPGKK196 pKa = 9.19GSVGEE201 pKa = 4.43GSLSAYY207 pKa = 9.31TGGAGGNVGAAGGRR221 pKa = 11.84CNTQGNGTEE230 pKa = 4.31YY231 pKa = 11.04NGGAAGKK238 pKa = 9.64AVTGNAPRR246 pKa = 11.84WDD248 pKa = 3.75KK249 pKa = 11.58VGTIYY254 pKa = 10.69GARR257 pKa = 11.84VV258 pKa = 2.9

Molecular weight: 25.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

277

0

277

51456

25

1246

185.8

21.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.707 ± 0.193

1.32 ± 0.077

6.6 ± 0.108

7.445 ± 0.166

4.474 ± 0.103

6.143 ± 0.18

1.984 ± 0.085

6.981 ± 0.114

7.686 ± 0.16

7.325 ± 0.119

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.074 ± 0.087

5.263 ± 0.139

3.205 ± 0.085

3.094 ± 0.1

4.783 ± 0.105

5.58 ± 0.14

5.789 ± 0.156

6.905 ± 0.125

1.349 ± 0.055

4.295 ± 0.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski