Lactobacillus virus Lb338-1
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 199 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C1KFD7|C1KFD7_9CAUD Uncharacterized protein OS=Lactobacillus virus Lb338-1 OX=632112 GN=lb338_phage_27 PE=4 SV=1
MM1 pKa = 7.23 SHH3 pKa = 7.34 KK4 pKa = 10.27 LTDD7 pKa = 3.72 NEE9 pKa = 4.16 HH10 pKa = 7.34 DD11 pKa = 3.93 SLKK14 pKa = 11.0 LSVLEE19 pKa = 4.39 QAGVDD24 pKa = 3.29 NWIGYY29 pKa = 7.77 SAVGAVYY36 pKa = 10.09 DD37 pKa = 4.22 YY38 pKa = 11.66 EE39 pKa = 6.16 DD40 pKa = 3.87 YY41 pKa = 11.23 LYY43 pKa = 11.35 GLDD46 pKa = 5.51 DD47 pKa = 4.81 DD48 pKa = 4.17 QTPLSFYY55 pKa = 11.01 EE56 pKa = 4.15 WLDD59 pKa = 3.65 KK60 pKa = 11.24 NNKK63 pKa = 10.13 SIDD66 pKa = 3.38 DD67 pKa = 3.82 WLL69 pKa = 5.72
Molecular weight: 8.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.872
IPC_protein 3.834
Toseland 3.617
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.528
Solomon 3.821
Lehninger 3.783
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.961
Patrickios 0.846
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|C1KFF9|C1KFF9_9CAUD Transposase OS=Lactobacillus virus Lb338-1 OX=632112 GN=lb338_phage_49 PE=3 SV=1
MM1 pKa = 7.55 GSASYY6 pKa = 11.06 CDD8 pKa = 3.1 IRR10 pKa = 11.84 HH11 pKa = 4.76 RR12 pKa = 11.84 TGWVGNRR19 pKa = 11.84 RR20 pKa = 11.84 PVGNKK25 pKa = 8.38 PASPCGSSWGNTGTLQLPP43 pKa = 4.17
Molecular weight: 4.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.294
IPC2_protein 9.37
IPC_protein 10.043
Toseland 10.028
ProMoST 9.882
Dawson 10.262
Bjellqvist 10.087
Wikipedia 10.482
Rodwell 10.379
Grimsley 10.335
Solomon 10.409
Lehninger 10.394
Nozaki 10.248
DTASelect 10.028
Thurlkill 10.131
EMBOSS 10.452
Sillero 10.248
Patrickios 10.496
IPC_peptide 10.394
IPC2_peptide 9.663
IPC2.peptide.svr19 8.129
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
199
0
199
42470
32
2222
213.4
24.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.388 ± 0.427
0.716 ± 0.082
6.981 ± 0.187
5.745 ± 0.276
3.563 ± 0.125
6.113 ± 0.198
1.754 ± 0.102
6.313 ± 0.154
8.585 ± 0.381
8.208 ± 0.226
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.428 ± 0.107
5.978 ± 0.172
3.212 ± 0.115
3.786 ± 0.178
3.52 ± 0.167
7.725 ± 0.289
6.11 ± 0.387
6.442 ± 0.144
1.187 ± 0.069
5.246 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here