Glutamicibacter arilaitensis (strain DSM 16368 / CIP 108037 / IAM 15318 / JCM 13566 / NCIMB 14258 / Re117) (Arthrobacter arilaitensis)
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3380 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E1VT29|E1VT29_GLUAR ESAT-6-like protein OS=Glutamicibacter arilaitensis (strain DSM 16368 / CIP 108037 / IAM 15318 / JCM 13566 / NCIMB 14258 / Re117) OX=861360 GN=AARI_05540 PE=3 SV=1
MM1 pKa = 7.01 MRR3 pKa = 11.84 LAAVGTALAMGLAGCSSSTADD24 pKa = 4.01 SQPEE28 pKa = 4.13 DD29 pKa = 3.72 AQSSQPAAKK38 pKa = 10.16 SSAPEE43 pKa = 4.06 GLEE46 pKa = 3.72 SFYY49 pKa = 10.68 TQEE52 pKa = 5.27 LDD54 pKa = 3.07 WEE56 pKa = 4.55 MCGSIIEE63 pKa = 4.41 CATIKK68 pKa = 10.88 VPMDD72 pKa = 3.34 YY73 pKa = 11.1 ANPEE77 pKa = 4.02 GQSIDD82 pKa = 3.31 LALNRR87 pKa = 11.84 RR88 pKa = 11.84 AVDD91 pKa = 3.47 GATGNILVNPGGPGGSGLDD110 pKa = 3.54 MVSSTVPSMFSNDD123 pKa = 2.87 LQRR126 pKa = 11.84 AYY128 pKa = 11.01 NVIGFDD134 pKa = 3.42 PRR136 pKa = 11.84 GVGEE140 pKa = 4.41 SSPVTCQSAAEE151 pKa = 4.12 TDD153 pKa = 3.09 KK154 pKa = 11.33 GRR156 pKa = 11.84 QEE158 pKa = 4.1 NLRR161 pKa = 11.84 AWVPEE166 pKa = 3.99 DD167 pKa = 2.97 QHH169 pKa = 7.57 EE170 pKa = 4.64 IIEE173 pKa = 4.4 STEE176 pKa = 4.36 DD177 pKa = 3.49 YY178 pKa = 11.29 AADD181 pKa = 3.76 CASNTGEE188 pKa = 4.77 LLDD191 pKa = 4.89 HH192 pKa = 6.93 VDD194 pKa = 3.58 TVSAARR200 pKa = 11.84 DD201 pKa = 3.38 MDD203 pKa = 4.24 VIRR206 pKa = 11.84 AVLGDD211 pKa = 3.7 AQLDD215 pKa = 4.06 YY216 pKa = 11.55 LGVSYY221 pKa = 8.33 GTFLGATYY229 pKa = 11.29 ADD231 pKa = 5.03 LFPQNVGRR239 pKa = 11.84 FVLDD243 pKa = 3.54 GAMDD247 pKa = 4.71 PSLPAAEE254 pKa = 4.27 VTLAQAVGFEE264 pKa = 4.43 KK265 pKa = 10.4 EE266 pKa = 3.6 TDD268 pKa = 2.89 AWLASCLEE276 pKa = 4.16 SDD278 pKa = 3.9 ACPFTGTVEE287 pKa = 3.98 EE288 pKa = 5.1 AKK290 pKa = 10.79 VQLQQFFAQVEE301 pKa = 4.37 NEE303 pKa = 4.16 PMTASDD309 pKa = 3.67 GRR311 pKa = 11.84 VVPIIDD317 pKa = 3.9 FVNGFVLPLYY327 pKa = 10.59 DD328 pKa = 4.49 DD329 pKa = 4.81 SSWSYY334 pKa = 10.02 LTDD337 pKa = 3.36 AMNAAINDD345 pKa = 3.82 ADD347 pKa = 3.39 VDD349 pKa = 4.36 MILGFADD356 pKa = 4.72 LSADD360 pKa = 3.92 RR361 pKa = 11.84 QSDD364 pKa = 3.44 GTYY367 pKa = 10.83 ASNSSDD373 pKa = 3.02 AFTAINCLDD382 pKa = 3.6 RR383 pKa = 11.84 PMDD386 pKa = 3.85 SSAEE390 pKa = 4.13 TMQDD394 pKa = 2.8 EE395 pKa = 4.68 ATEE398 pKa = 4.18 LMRR401 pKa = 11.84 MAPTLGKK408 pKa = 9.68 YY409 pKa = 9.21 LAYY412 pKa = 10.48 GSITCEE418 pKa = 3.25 AWDD421 pKa = 4.0 YY422 pKa = 11.67 DD423 pKa = 4.0 STGTPEE429 pKa = 4.46 ILDD432 pKa = 3.69 APGSNEE438 pKa = 3.39 ILVIGTVGDD447 pKa = 3.48 PATPYY452 pKa = 10.11 KK453 pKa = 10.18 WSEE456 pKa = 3.91 ALAKK460 pKa = 10.55 QLDD463 pKa = 3.86 NATLLTYY470 pKa = 10.08 EE471 pKa = 4.37 GHH473 pKa = 6.0 GHH475 pKa = 4.9 TAYY478 pKa = 10.32 GRR480 pKa = 11.84 SNEE483 pKa = 4.64 CITKK487 pKa = 10.54 AVDD490 pKa = 3.29 EE491 pKa = 4.2 YY492 pKa = 11.39 LIDD495 pKa = 4.11 GKK497 pKa = 11.08 VPEE500 pKa = 5.1 AGTRR504 pKa = 11.84 CC505 pKa = 3.49
Molecular weight: 53.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.834
IPC_protein 3.859
Toseland 3.643
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.757
Rodwell 3.681
Grimsley 3.541
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.177
Thurlkill 3.681
EMBOSS 3.77
Sillero 3.973
Patrickios 1.43
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.858
Protein with the highest isoelectric point:
>tr|E1VRE3|E1VRE3_GLUAR Uncharacterized protein OS=Glutamicibacter arilaitensis (strain DSM 16368 / CIP 108037 / IAM 15318 / JCM 13566 / NCIMB 14258 / Re117) OX=861360 GN=AARI_pI00410 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SKK15 pKa = 10.39 KK16 pKa = 9.03 HH17 pKa = 4.36 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 9.94 NRR40 pKa = 11.84 AEE42 pKa = 3.99 LSAA45 pKa = 5.01
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.44
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.112
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3380
0
3380
1062228
30
2173
314.3
34.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.065 ± 0.063
0.659 ± 0.011
5.42 ± 0.037
6.274 ± 0.041
3.317 ± 0.028
8.174 ± 0.036
2.175 ± 0.02
5.091 ± 0.029
3.516 ± 0.028
10.331 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.246 ± 0.017
2.89 ± 0.029
4.804 ± 0.032
3.782 ± 0.023
5.952 ± 0.048
6.343 ± 0.03
5.683 ± 0.034
7.68 ± 0.034
1.409 ± 0.019
2.19 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here