Natronococcus occultus SP4

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Natronococcus; Natronococcus occultus

Average proteome isoelectric point is 4.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0K0A1|L0K0A1_9EURY Putative signal-transduction protein containing cAMP-binding and CBS domains OS=Natronococcus occultus SP4 OX=694430 GN=Natoc_1984 PE=4 SV=1
MM1 pKa = 6.69STEE4 pKa = 3.89TEE6 pKa = 3.9RR7 pKa = 11.84AEE9 pKa = 3.95EE10 pKa = 4.04RR11 pKa = 11.84TEE13 pKa = 4.24SSVLGSWAEE22 pKa = 4.12WYY24 pKa = 9.41HH25 pKa = 6.25VPVVGAVMLFMFWVRR40 pKa = 11.84TQSYY44 pKa = 9.04EE45 pKa = 4.1AFLTEE50 pKa = 5.42DD51 pKa = 3.74GTPALAGIDD60 pKa = 3.44SWYY63 pKa = 10.16HH64 pKa = 4.39WRR66 pKa = 11.84TIQWTAEE73 pKa = 4.09NYY75 pKa = 7.06PQTMPYY81 pKa = 9.7EE82 pKa = 4.28VWTGFPTGNYY92 pKa = 8.6VGQFGTLFDD101 pKa = 3.92QLIVTAAMIVGLGDD115 pKa = 3.72PSSSTLYY122 pKa = 9.84TVSLFAVPAMAALVAIPVFYY142 pKa = 10.72LGRR145 pKa = 11.84RR146 pKa = 11.84LGGTVGGLVAVVLLALAPGQFLVRR170 pKa = 11.84TTAGQLQHH178 pKa = 6.65HH179 pKa = 5.69VAEE182 pKa = 4.37VLFMALAVLGMAVALRR198 pKa = 11.84VAEE201 pKa = 4.12RR202 pKa = 11.84EE203 pKa = 3.94QPIYY207 pKa = 10.75EE208 pKa = 4.18LAADD212 pKa = 4.58RR213 pKa = 11.84NWAPLRR219 pKa = 11.84EE220 pKa = 4.08PTLYY224 pKa = 10.73SVLAGVALALYY235 pKa = 9.39IWVWPPGVVLIGIFGVFFAIQLCLDD260 pKa = 3.77YY261 pKa = 11.36VRR263 pKa = 11.84GISPDD268 pKa = 2.92HH269 pKa = 5.9VAFVGAVSLGVTAVATALLIEE290 pKa = 4.43EE291 pKa = 5.33PGTSVTSFGYY301 pKa = 9.58LQPGTAALVAVGCLFMAWFARR322 pKa = 11.84QWNARR327 pKa = 11.84GIDD330 pKa = 3.13RR331 pKa = 11.84RR332 pKa = 11.84LYY334 pKa = 9.61PGAIAALIVGTFAVMWLVLPEE355 pKa = 4.6FYY357 pKa = 9.4DD358 pKa = 3.7TLVGNLTGRR367 pKa = 11.84IIPLDD372 pKa = 4.03PDD374 pKa = 3.83AGTLTIQEE382 pKa = 4.32AQPPEE387 pKa = 4.97DD388 pKa = 3.59FTAHH392 pKa = 5.59VFDD395 pKa = 4.31EE396 pKa = 5.08FGTAFYY402 pKa = 10.89TMLAGLVLLAARR414 pKa = 11.84PLLGRR419 pKa = 11.84EE420 pKa = 3.73HH421 pKa = 6.65RR422 pKa = 11.84AEE424 pKa = 3.95YY425 pKa = 10.16TLILVWSLFLLSMSATQIRR444 pKa = 11.84FSYY447 pKa = 10.41YY448 pKa = 10.04LVLAVAVVNAAFVADD463 pKa = 3.76VLRR466 pKa = 11.84LFDD469 pKa = 5.85LDD471 pKa = 3.93LQRR474 pKa = 11.84GTQALRR480 pKa = 11.84GIEE483 pKa = 4.13TYY485 pKa = 10.58QIIILFVVVVLLFAPLLPVVAADD508 pKa = 3.43GDD510 pKa = 4.29TAVEE514 pKa = 4.18RR515 pKa = 11.84GEE517 pKa = 4.18ATGPHH522 pKa = 7.11PDD524 pKa = 3.05AMVWEE529 pKa = 5.15EE530 pKa = 4.06STHH533 pKa = 4.66WLEE536 pKa = 5.15ANTPEE541 pKa = 4.45PGNYY545 pKa = 9.75GGADD549 pKa = 3.31YY550 pKa = 11.25ADD552 pKa = 3.56EE553 pKa = 4.44LEE555 pKa = 4.65YY556 pKa = 11.07YY557 pKa = 10.65GSYY560 pKa = 10.5DD561 pKa = 3.61YY562 pKa = 11.45PEE564 pKa = 6.41DD565 pKa = 4.22GDD567 pKa = 3.71FDD569 pKa = 4.51YY570 pKa = 10.48PAGAYY575 pKa = 10.04GVMSWWDD582 pKa = 3.65YY583 pKa = 10.73GHH585 pKa = 7.77LITTQAEE592 pKa = 4.61RR593 pKa = 11.84IPHH596 pKa = 5.98SNPFQQNAEE605 pKa = 4.12TASAYY610 pKa = 8.42LTAEE614 pKa = 4.24SEE616 pKa = 4.44RR617 pKa = 11.84EE618 pKa = 3.85AEE620 pKa = 4.86LILEE624 pKa = 5.07AIAAGEE630 pKa = 4.56DD631 pKa = 4.01PLDD634 pKa = 4.7DD635 pKa = 5.55DD636 pKa = 5.97DD637 pKa = 4.79NVRR640 pKa = 11.84PLEE643 pKa = 4.04EE644 pKa = 5.45LEE646 pKa = 4.11TAIEE650 pKa = 4.47DD651 pKa = 4.72DD652 pKa = 3.73ADD654 pKa = 3.65EE655 pKa = 3.98QLRR658 pKa = 11.84YY659 pKa = 11.03VMIDD663 pKa = 3.2DD664 pKa = 3.69QMAGGKK670 pKa = 9.66FSAMTAWSGPDD681 pKa = 3.01YY682 pKa = 11.07DD683 pKa = 5.3HH684 pKa = 7.05YY685 pKa = 10.65VTPDD689 pKa = 3.35VEE691 pKa = 4.81PGEE694 pKa = 4.61SIDD697 pKa = 5.76RR698 pKa = 11.84EE699 pKa = 4.26DD700 pKa = 3.28VDD702 pKa = 5.78DD703 pKa = 4.3RR704 pKa = 11.84LKK706 pKa = 11.15VPYY709 pKa = 9.62EE710 pKa = 3.87EE711 pKa = 4.52TMVADD716 pKa = 5.51LYY718 pKa = 11.37FDD720 pKa = 4.88DD721 pKa = 4.32ATGMDD726 pKa = 4.47HH727 pKa = 6.81YY728 pKa = 11.32RR729 pKa = 11.84LVHH732 pKa = 6.24EE733 pKa = 4.53NDD735 pKa = 2.79EE736 pKa = 4.27RR737 pKa = 11.84TAGFVSYY744 pKa = 11.29ALIDD748 pKa = 3.99PEE750 pKa = 4.23TDD752 pKa = 2.91EE753 pKa = 4.89VLTDD757 pKa = 3.21EE758 pKa = 4.72TGQHH762 pKa = 4.76QVAFNQPLQQAEE774 pKa = 4.33VQLSQFEE781 pKa = 4.09QSPDD785 pKa = 3.0VDD787 pKa = 3.45VEE789 pKa = 4.36YY790 pKa = 10.6IDD792 pKa = 5.19DD793 pKa = 4.18RR794 pKa = 11.84EE795 pKa = 4.21TAGVKK800 pKa = 9.13TYY802 pKa = 10.74EE803 pKa = 4.18RR804 pKa = 11.84VAGATIVGTADD815 pKa = 3.21ADD817 pKa = 3.74ADD819 pKa = 3.87EE820 pKa = 5.38GDD822 pKa = 3.92VVTAEE827 pKa = 4.71LEE829 pKa = 4.25LDD831 pKa = 3.48SGTDD835 pKa = 2.97RR836 pKa = 11.84GSFTYY841 pKa = 10.09EE842 pKa = 3.56QEE844 pKa = 4.38TEE846 pKa = 3.65VDD848 pKa = 3.1ADD850 pKa = 4.14GEE852 pKa = 4.5FEE854 pKa = 4.41LTVPYY859 pKa = 9.72ATDD862 pKa = 3.91DD863 pKa = 3.78EE864 pKa = 6.09LDD866 pKa = 3.66VEE868 pKa = 4.8DD869 pKa = 6.14GYY871 pKa = 9.87TEE873 pKa = 4.24SAVEE877 pKa = 4.05AVDD880 pKa = 4.86DD881 pKa = 3.89YY882 pKa = 11.29TVTVEE887 pKa = 5.07GEE889 pKa = 4.32TPQEE893 pKa = 3.91GDD895 pKa = 3.67LEE897 pKa = 4.6VPEE900 pKa = 4.41SAVVDD905 pKa = 4.1GEE907 pKa = 4.55TVEE910 pKa = 4.58VEE912 pKa = 4.18LEE914 pKa = 4.01EE915 pKa = 4.63TEE917 pKa = 5.93LEE919 pKa = 4.19DD920 pKa = 5.47AEE922 pKa = 4.64NDD924 pKa = 3.68GATEE928 pKa = 4.65DD929 pKa = 4.03EE930 pKa = 4.53TDD932 pKa = 4.73GDD934 pKa = 4.1DD935 pKa = 4.72ADD937 pKa = 3.99AAEE940 pKa = 5.21ADD942 pKa = 4.17TEE944 pKa = 4.44TADD947 pKa = 4.89EE948 pKa = 4.85DD949 pKa = 5.04ADD951 pKa = 4.03DD952 pKa = 4.93AAANDD957 pKa = 3.73ADD959 pKa = 4.3AEE961 pKa = 4.32AGADD965 pKa = 3.97DD966 pKa = 5.59AGADD970 pKa = 3.84DD971 pKa = 4.57TASSLAPAAGLADD984 pKa = 3.56

Molecular weight: 107.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0JT47|L0JT47_9EURY NH(3)-dependent NAD(+) synthetase OS=Natronococcus occultus SP4 OX=694430 GN=nadE PE=3 SV=1
MM1 pKa = 7.38PVVSVSMPEE10 pKa = 3.81EE11 pKa = 3.67LLEE14 pKa = 4.6RR15 pKa = 11.84LDD17 pKa = 3.85AHH19 pKa = 6.91ASAHH23 pKa = 6.47EE24 pKa = 4.04YY25 pKa = 9.2TGRR28 pKa = 11.84SEE30 pKa = 4.46VVRR33 pKa = 11.84EE34 pKa = 4.0STRR37 pKa = 11.84ALLEE41 pKa = 4.07AFTDD45 pKa = 3.86DD46 pKa = 3.99RR47 pKa = 11.84VEE49 pKa = 4.72DD50 pKa = 3.89GALAGTVDD58 pKa = 2.98VRR60 pKa = 11.84YY61 pKa = 10.1DD62 pKa = 3.17VGTPSVEE69 pKa = 3.62RR70 pKa = 11.84RR71 pKa = 11.84VAALRR76 pKa = 11.84HH77 pKa = 5.49EE78 pKa = 4.49FDD80 pKa = 3.57EE81 pKa = 5.06AVAATDD87 pKa = 3.49HH88 pKa = 6.24SHH90 pKa = 7.0VGDD93 pKa = 3.82YY94 pKa = 11.24CPDD97 pKa = 3.74LFVLEE102 pKa = 4.73ADD104 pKa = 4.1RR105 pKa = 11.84EE106 pKa = 4.65TISSVVDD113 pKa = 2.88KK114 pKa = 10.52CGRR117 pKa = 11.84STEE120 pKa = 4.26PRR122 pKa = 11.84TSTTRR127 pKa = 11.84SSSSTADD134 pKa = 2.61RR135 pKa = 11.84VVHH138 pKa = 5.63SKK140 pKa = 10.42RR141 pKa = 11.84RR142 pKa = 11.84SHH144 pKa = 6.89PNAPIAARR152 pKa = 11.84RR153 pKa = 11.84GSVAGGKK160 pKa = 8.35VSRR163 pKa = 11.84LRR165 pKa = 11.84LLSLEE170 pKa = 4.3SRR172 pKa = 11.84WFEE175 pKa = 3.94CEE177 pKa = 3.47TRR179 pKa = 11.84SRR181 pKa = 11.84IPCHH185 pKa = 6.56RR186 pKa = 11.84PIPHH190 pKa = 6.91PPIPIRR196 pKa = 11.84SSPVRR201 pKa = 11.84PPQSGRR207 pKa = 11.84TVPRR211 pKa = 11.84TKK213 pKa = 10.4KK214 pKa = 10.07PSSTTSTVRR223 pKa = 11.84KK224 pKa = 9.23RR225 pKa = 11.84GSARR229 pKa = 11.84RR230 pKa = 11.84TPWRR234 pKa = 11.84SPTSNEE240 pKa = 3.94TGGSDD245 pKa = 3.29TASRR249 pKa = 11.84AQRR252 pKa = 11.84RR253 pKa = 11.84TSRR256 pKa = 11.84ISNPSRR262 pKa = 11.84VLSAWW267 pKa = 3.14

Molecular weight: 29.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4153

0

4153

1196774

29

1804

288.2

31.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.654 ± 0.057

0.765 ± 0.013

8.453 ± 0.054

9.608 ± 0.054

3.195 ± 0.027

8.314 ± 0.038

1.982 ± 0.017

4.288 ± 0.029

1.726 ± 0.026

9.13 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.697 ± 0.016

2.196 ± 0.018

4.651 ± 0.024

2.35 ± 0.022

6.656 ± 0.042

5.331 ± 0.03

6.262 ± 0.026

8.829 ± 0.037

1.151 ± 0.014

2.763 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski