Novosphingobium sp. ST904

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4594 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1MVY3|A0A0N1MVY3_9SPHN Histidinol dehydrogenase OS=Novosphingobium sp. ST904 OX=1684385 GN=ADT71_07640 PE=3 SV=1
MM1 pKa = 7.31LRR3 pKa = 11.84KK4 pKa = 9.19IALSAAGLSAIVLATNPARR23 pKa = 11.84ADD25 pKa = 3.12ITGTIDD31 pKa = 3.19ATITLEE37 pKa = 4.26AGCIVNGQNYY47 pKa = 10.49DD48 pKa = 3.59DD49 pKa = 4.15GTANVAFGTLDD60 pKa = 3.35FGTQNTLFTQADD72 pKa = 3.75AQVLNGGAAFTVQCSNGIAPTLSFNAGQNDD102 pKa = 3.97GSGAGVGVHH111 pKa = 6.8AMANQTAAGQFVTYY125 pKa = 10.85NLYY128 pKa = 10.74SDD130 pKa = 3.92TNRR133 pKa = 11.84SVIIPVDD140 pKa = 3.51GDD142 pKa = 3.29ITLPADD148 pKa = 3.82GTQQTVNVYY157 pKa = 9.82GRR159 pKa = 11.84AFGEE163 pKa = 4.34AGLTTGTYY171 pKa = 9.55SDD173 pKa = 3.79VVTVVLEE180 pKa = 3.99LL181 pKa = 3.87

Molecular weight: 18.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N0M221|A0A0N0M221_9SPHN Pilus assembly protein TadC OS=Novosphingobium sp. ST904 OX=1684385 GN=ADT71_09535 PE=4 SV=1
MM1 pKa = 7.5GARR4 pKa = 11.84LRR6 pKa = 11.84AIPAFAGTTKK16 pKa = 10.37KK17 pKa = 10.54VALRR21 pKa = 11.84LATPGRR27 pKa = 11.84TKK29 pKa = 10.55LWGRR33 pKa = 11.84VLRR36 pKa = 11.84GGGARR41 pKa = 11.84RR42 pKa = 11.84KK43 pKa = 9.69GAGSARR49 pKa = 11.84AMALALRR56 pKa = 11.84FLLPWLRR63 pKa = 11.84MSRR66 pKa = 11.84PWPVTILPSALRR78 pKa = 11.84QAWGSLL84 pKa = 3.35

Molecular weight: 9.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4594

0

4594

1316690

29

1580

286.6

31.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.934 ± 0.046

0.852 ± 0.011

5.783 ± 0.028

5.671 ± 0.035

3.565 ± 0.024

8.917 ± 0.039

2.036 ± 0.017

4.797 ± 0.025

3.017 ± 0.027

9.912 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.019

2.545 ± 0.023

5.288 ± 0.028

3.189 ± 0.021

7.371 ± 0.036

5.538 ± 0.027

5.239 ± 0.029

7.115 ± 0.028

1.483 ± 0.018

2.253 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski