Sewage-associated circular DNA molecule

Taxonomy: Viruses; Satellites; DNA satellites

Average proteome isoelectric point is 7.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UG03|A0A0B4UG03_9VIRU Replication-associated protein OS=Sewage-associated circular DNA molecule OX=1592207 PE=4 SV=1
MM1 pKa = 7.94SYY3 pKa = 10.97RR4 pKa = 11.84NWCFTLNNPIMNDD17 pKa = 3.17GKK19 pKa = 11.09VIAAFADD26 pKa = 3.93YY27 pKa = 11.55ANIRR31 pKa = 11.84YY32 pKa = 8.63LVFQLEE38 pKa = 4.4VGEE41 pKa = 4.75LGTPHH46 pKa = 6.06SQGYY50 pKa = 9.82CEE52 pKa = 3.89FTKK55 pKa = 10.33PVRR58 pKa = 11.84LVALKK63 pKa = 10.38KK64 pKa = 10.14IIPGVHH70 pKa = 5.66GEE72 pKa = 4.1PRR74 pKa = 11.84KK75 pKa = 8.75GTRR78 pKa = 11.84DD79 pKa = 3.23QARR82 pKa = 11.84AYY84 pKa = 10.38CMKK87 pKa = 10.27EE88 pKa = 3.77DD89 pKa = 3.96TRR91 pKa = 11.84TEE93 pKa = 4.35GPWEE97 pKa = 3.96YY98 pKa = 11.4GEE100 pKa = 4.73WPLQQGKK107 pKa = 10.13RR108 pKa = 11.84SDD110 pKa = 3.71VDD112 pKa = 3.81VVIADD117 pKa = 3.8INAGKK122 pKa = 10.17SLADD126 pKa = 3.43IQQDD130 pKa = 3.2HH131 pKa = 6.46PAIYY135 pKa = 9.79IRR137 pKa = 11.84LHH139 pKa = 6.61AGIEE143 pKa = 3.95KK144 pKa = 10.54LIAARR149 pKa = 11.84KK150 pKa = 7.2PVKK153 pKa = 9.59PVPKK157 pKa = 9.65IYY159 pKa = 10.11WFWGPSGTGKK169 pKa = 8.18TRR171 pKa = 11.84EE172 pKa = 4.32AKK174 pKa = 10.41RR175 pKa = 11.84NAQGEE180 pKa = 4.42DD181 pKa = 3.35DD182 pKa = 4.12VLIDD186 pKa = 3.45GTDD189 pKa = 3.56YY190 pKa = 11.5AFSSPPPRR198 pKa = 11.84IFQEE202 pKa = 4.2YY203 pKa = 8.94KK204 pKa = 9.04SHH206 pKa = 6.51IKK208 pKa = 10.51RR209 pKa = 11.84FILDD213 pKa = 3.48EE214 pKa = 4.16YY215 pKa = 8.45HH216 pKa = 6.96PKK218 pKa = 9.94MIPHH222 pKa = 7.11HH223 pKa = 5.44LLKK226 pKa = 10.4RR227 pKa = 11.84YY228 pKa = 9.35IDD230 pKa = 3.79RR231 pKa = 11.84NPVVVNVMYY240 pKa = 8.85GTEE243 pKa = 3.97HH244 pKa = 6.61FVPEE248 pKa = 4.87EE249 pKa = 3.82IYY251 pKa = 9.18ITCSVPPSHH260 pKa = 7.29FWTGNPLRR268 pKa = 11.84EE269 pKa = 3.94IEE271 pKa = 4.23RR272 pKa = 11.84RR273 pKa = 11.84CTEE276 pKa = 3.22IRR278 pKa = 11.84EE279 pKa = 4.29FKK281 pKa = 10.89

Molecular weight: 32.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UG03|A0A0B4UG03_9VIRU Replication-associated protein OS=Sewage-associated circular DNA molecule OX=1592207 PE=4 SV=1
MM1 pKa = 7.94SYY3 pKa = 10.97RR4 pKa = 11.84NWCFTLNNPIMNDD17 pKa = 3.17GKK19 pKa = 11.09VIAAFADD26 pKa = 3.93YY27 pKa = 11.55ANIRR31 pKa = 11.84YY32 pKa = 8.63LVFQLEE38 pKa = 4.4VGEE41 pKa = 4.75LGTPHH46 pKa = 6.06SQGYY50 pKa = 9.82CEE52 pKa = 3.89FTKK55 pKa = 10.33PVRR58 pKa = 11.84LVALKK63 pKa = 10.38KK64 pKa = 10.14IIPGVHH70 pKa = 5.66GEE72 pKa = 4.1PRR74 pKa = 11.84KK75 pKa = 8.75GTRR78 pKa = 11.84DD79 pKa = 3.23QARR82 pKa = 11.84AYY84 pKa = 10.38CMKK87 pKa = 10.27EE88 pKa = 3.77DD89 pKa = 3.96TRR91 pKa = 11.84TEE93 pKa = 4.35GPWEE97 pKa = 3.96YY98 pKa = 11.4GEE100 pKa = 4.73WPLQQGKK107 pKa = 10.13RR108 pKa = 11.84SDD110 pKa = 3.71VDD112 pKa = 3.81VVIADD117 pKa = 3.8INAGKK122 pKa = 10.17SLADD126 pKa = 3.43IQQDD130 pKa = 3.2HH131 pKa = 6.46PAIYY135 pKa = 9.79IRR137 pKa = 11.84LHH139 pKa = 6.61AGIEE143 pKa = 3.95KK144 pKa = 10.54LIAARR149 pKa = 11.84KK150 pKa = 7.2PVKK153 pKa = 9.59PVPKK157 pKa = 9.65IYY159 pKa = 10.11WFWGPSGTGKK169 pKa = 8.18TRR171 pKa = 11.84EE172 pKa = 4.32AKK174 pKa = 10.41RR175 pKa = 11.84NAQGEE180 pKa = 4.42DD181 pKa = 3.35DD182 pKa = 4.12VLIDD186 pKa = 3.45GTDD189 pKa = 3.56YY190 pKa = 11.5AFSSPPPRR198 pKa = 11.84IFQEE202 pKa = 4.2YY203 pKa = 8.94KK204 pKa = 9.04SHH206 pKa = 6.51IKK208 pKa = 10.51RR209 pKa = 11.84FILDD213 pKa = 3.48EE214 pKa = 4.16YY215 pKa = 8.45HH216 pKa = 6.96PKK218 pKa = 9.94MIPHH222 pKa = 7.11HH223 pKa = 5.44LLKK226 pKa = 10.4RR227 pKa = 11.84YY228 pKa = 9.35IDD230 pKa = 3.79RR231 pKa = 11.84NPVVVNVMYY240 pKa = 8.85GTEE243 pKa = 3.97HH244 pKa = 6.61FVPEE248 pKa = 4.87EE249 pKa = 3.82IYY251 pKa = 9.18ITCSVPPSHH260 pKa = 7.29FWTGNPLRR268 pKa = 11.84EE269 pKa = 3.94IEE271 pKa = 4.23RR272 pKa = 11.84RR273 pKa = 11.84CTEE276 pKa = 3.22IRR278 pKa = 11.84EE279 pKa = 4.29FKK281 pKa = 10.89

Molecular weight: 32.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

281

281

281

281.0

32.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.05 ± 0.0

1.779 ± 0.0

5.338 ± 0.0

7.117 ± 0.0

3.915 ± 0.0

6.762 ± 0.0

3.559 ± 0.0

8.185 ± 0.0

6.762 ± 0.0

5.338 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.779 ± 0.0

3.559 ± 0.0

7.829 ± 0.0

3.203 ± 0.0

7.117 ± 0.0

3.559 ± 0.0

4.626 ± 0.0

6.406 ± 0.0

2.135 ± 0.0

4.982 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski