Synechococcus virus S-ESS1
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V0DX24|A0A1V0DX24_9CAUD Terminase large subunit OS=Synechococcus virus S-ESS1 OX=1964565 PE=3 SV=1
MM1 pKa = 7.38 ARR3 pKa = 11.84 NYY5 pKa = 9.69 TLGRR9 pKa = 11.84 GKK11 pKa = 10.19 VHH13 pKa = 7.05 FARR16 pKa = 11.84 FKK18 pKa = 10.82 PGTQVPDD25 pKa = 3.28 GFFYY29 pKa = 10.49 IGNTPEE35 pKa = 3.7 FSLTIEE41 pKa = 4.43 SEE43 pKa = 4.41 SLDD46 pKa = 3.56 HH47 pKa = 6.9 FSSDD51 pKa = 2.72 EE52 pKa = 4.36 GIRR55 pKa = 11.84 EE56 pKa = 4.0 KK57 pKa = 11.01 DD58 pKa = 3.24 DD59 pKa = 3.95 SVPLEE64 pKa = 4.02 VNRR67 pKa = 11.84 TGSLTTDD74 pKa = 3.51 NIDD77 pKa = 3.73 PEE79 pKa = 4.32 NVALFFFGSSSVITQAAVASATEE102 pKa = 4.04 TLTGIKK108 pKa = 10.01 AGHH111 pKa = 5.9 SYY113 pKa = 11.16 KK114 pKa = 10.8 LGVGPTNPAGYY125 pKa = 8.89 MGIDD129 pKa = 3.22 TTGFTVEE136 pKa = 4.15 VGVTPLVVDD145 pKa = 3.65 VDD147 pKa = 3.79 YY148 pKa = 11.76 TMDD151 pKa = 3.81 FDD153 pKa = 4.27 NGVITFLPDD162 pKa = 2.88 SVLAVDD168 pKa = 4.2 AADD171 pKa = 3.41 VDD173 pKa = 3.94 VTYY176 pKa = 11.07 AVAASTRR183 pKa = 11.84 SRR185 pKa = 11.84 VISGSEE191 pKa = 3.83 PVEE194 pKa = 3.83 GAMMYY199 pKa = 8.36 ITKK202 pKa = 10.19 NPKK205 pKa = 8.79 GTDD208 pKa = 3.14 CTFYY212 pKa = 9.78 MPYY215 pKa = 10.12 VKK217 pKa = 10.55 VMPNGDD223 pKa = 3.56 YY224 pKa = 11.02 ALKK227 pKa = 10.41 GDD229 pKa = 4.09 EE230 pKa = 4.11 WQQIPLALEE239 pKa = 3.8 VLKK242 pKa = 10.4 PLTGEE247 pKa = 4.33 AIYY250 pKa = 10.73 RR251 pKa = 11.84 DD252 pKa = 4.28 GTPTMTPP259 pKa = 3.1
Molecular weight: 27.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.366
IPC2_protein 4.355
IPC_protein 4.317
Toseland 4.126
ProMoST 4.444
Dawson 4.279
Bjellqvist 4.431
Wikipedia 4.19
Rodwell 4.139
Grimsley 4.037
Solomon 4.279
Lehninger 4.24
Nozaki 4.393
DTASelect 4.609
Thurlkill 4.151
EMBOSS 4.202
Sillero 4.431
Patrickios 3.592
IPC_peptide 4.279
IPC2_peptide 4.418
IPC2.peptide.svr19 4.358
Protein with the highest isoelectric point:
>tr|A0A1V0DX53|A0A1V0DX53_9CAUD DUF3310 domain-containing protein OS=Synechococcus virus S-ESS1 OX=1964565 PE=4 SV=1
MM1 pKa = 6.99 KK2 pKa = 9.86 TVFSNHH8 pKa = 4.43 MVAHH12 pKa = 5.9 VFATQSQEE20 pKa = 3.7 EE21 pKa = 4.59 GRR23 pKa = 11.84 SQNGNFYY30 pKa = 10.63 FSGRR34 pKa = 11.84 ALFSYY39 pKa = 10.06 GRR41 pKa = 11.84 HH42 pKa = 5.98 FIVAYY47 pKa = 9.46 AIPRR51 pKa = 11.84 ISGGFLFLINPDD63 pKa = 3.54 SYY65 pKa = 11.61 SISTSRR71 pKa = 11.84 HH72 pKa = 3.24 QAMARR77 pKa = 11.84 AAIPGRR83 pKa = 11.84 AYY85 pKa = 9.78 IAPNLTRR92 pKa = 11.84 LADD95 pKa = 4.28 DD96 pKa = 4.77 LNAALDD102 pKa = 3.19 AWEE105 pKa = 3.99 YY106 pKa = 11.38 HH107 pKa = 5.65 EE108 pKa = 4.74 MPGGRR113 pKa = 11.84 TRR115 pKa = 11.84 PVKK118 pKa = 10.4 VAATMARR125 pKa = 11.84 RR126 pKa = 11.84 RR127 pKa = 11.84 EE128 pKa = 3.83 YY129 pKa = 10.78 LPRR132 pKa = 11.84 IKK134 pKa = 10.34 RR135 pKa = 11.84 AIAANWPGQEE145 pKa = 3.55 TAAAILGEE153 pKa = 4.3 FGQRR157 pKa = 11.84 NAARR161 pKa = 11.84 AAAAMARR168 pKa = 11.84 RR169 pKa = 11.84 HH170 pKa = 5.56 AATVAAEE177 pKa = 4.14 AAAKK181 pKa = 10.17 AKK183 pKa = 10.33 RR184 pKa = 11.84 KK185 pKa = 10.18 RR186 pKa = 11.84 EE187 pKa = 3.92 DD188 pKa = 2.92 VARR191 pKa = 11.84 NAKK194 pKa = 9.71 YY195 pKa = 10.31 LATFTPARR203 pKa = 11.84 IRR205 pKa = 11.84 AEE207 pKa = 3.47 ISDD210 pKa = 3.58 NLNRR214 pKa = 11.84 YY215 pKa = 8.18 SGQWGRR221 pKa = 11.84 DD222 pKa = 3.04 KK223 pKa = 10.98 VKK225 pKa = 10.45 EE226 pKa = 4.02 MSRR229 pKa = 11.84 HH230 pKa = 4.83 LFAAIKK236 pKa = 9.14 EE237 pKa = 4.04 ARR239 pKa = 11.84 ARR241 pKa = 11.84 GWTRR245 pKa = 11.84 IAATLSSHH253 pKa = 6.38 RR254 pKa = 11.84 AMVRR258 pKa = 11.84 AALADD263 pKa = 3.38 IDD265 pKa = 4.15 NRR267 pKa = 11.84 EE268 pKa = 3.79 AMARR272 pKa = 11.84 RR273 pKa = 11.84 QGAKK277 pKa = 9.55 RR278 pKa = 11.84 EE279 pKa = 4.18 AIARR283 pKa = 11.84 FRR285 pKa = 11.84 EE286 pKa = 4.12 DD287 pKa = 2.89 AANVARR293 pKa = 11.84 LMTPRR298 pKa = 11.84 DD299 pKa = 3.32 AGGIAGEE306 pKa = 4.42 GSPEE310 pKa = 3.76 TRR312 pKa = 11.84 ARR314 pKa = 11.84 WYY316 pKa = 10.61 RR317 pKa = 11.84 DD318 pKa = 3.17 LADD321 pKa = 3.88 SCHH324 pKa = 6.32 SLLRR328 pKa = 11.84 HH329 pKa = 5.24 WTPPAATVARR339 pKa = 11.84 LRR341 pKa = 11.84 SIHH344 pKa = 6.35 DD345 pKa = 3.45 AARR348 pKa = 11.84 IRR350 pKa = 11.84 MTEE353 pKa = 3.88 EE354 pKa = 3.47 NEE356 pKa = 3.59 NAARR360 pKa = 11.84 ARR362 pKa = 11.84 FEE364 pKa = 4.34 AEE366 pKa = 3.64 AEE368 pKa = 4.04 RR369 pKa = 11.84 RR370 pKa = 11.84 RR371 pKa = 11.84 AWLAGEE377 pKa = 4.18 TGLGYY382 pKa = 10.96 GRR384 pKa = 11.84 LSDD387 pKa = 3.73 QDD389 pKa = 3.29 GGALLRR395 pKa = 11.84 AVDD398 pKa = 3.73 VARR401 pKa = 11.84 DD402 pKa = 3.42 EE403 pKa = 4.56 SGAIVSGVLEE413 pKa = 4.52 TGHH416 pKa = 6.46 GAQVPLVHH424 pKa = 7.19 ALRR427 pKa = 11.84 VFAFLRR433 pKa = 11.84 HH434 pKa = 5.45 CRR436 pKa = 11.84 ATGRR440 pKa = 11.84 AWKK443 pKa = 10.44 RR444 pKa = 11.84 NGASLRR450 pKa = 11.84 VGHH453 pKa = 5.91 FHH455 pKa = 6.87 VDD457 pKa = 3.07 SVTPQGDD464 pKa = 4.08 FVAGCHH470 pKa = 6.18 RR471 pKa = 11.84 INWQEE476 pKa = 3.85 VARR479 pKa = 11.84 LAEE482 pKa = 4.14 SLGVAEE488 pKa = 5.35 LAPADD493 pKa = 3.98 TTEE496 pKa = 3.91 GHH498 pKa = 7.34 AIAA501 pKa = 5.47
Molecular weight: 55.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.604
IPC_protein 10.73
Toseland 10.701
ProMoST 10.657
Dawson 10.804
Bjellqvist 10.613
Wikipedia 11.111
Rodwell 10.76
Grimsley 10.862
Solomon 11.023
Lehninger 10.965
Nozaki 10.701
DTASelect 10.613
Thurlkill 10.716
EMBOSS 11.14
Sillero 10.745
Patrickios 10.423
IPC_peptide 11.023
IPC2_peptide 9.794
IPC2.peptide.svr19 8.707
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
52
0
52
13802
66
716
265.4
29.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.556 ± 0.777
0.935 ± 0.12
6.303 ± 0.276
7.303 ± 0.283
3.485 ± 0.131
7.847 ± 0.335
2.058 ± 0.198
4.847 ± 0.181
4.608 ± 0.287
7.593 ± 0.365
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.84 ± 0.158
3.253 ± 0.168
5.086 ± 0.391
3.739 ± 0.213
7.318 ± 0.353
5.688 ± 0.229
5.55 ± 0.269
6.427 ± 0.265
1.746 ± 0.134
2.818 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here