Capybara microvirus Cap3_SP_550
Average proteome isoelectric point is 5.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W532|A0A4P8W532_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_550 OX=2585474 PE=4 SV=1
MM1 pKa = 6.96 AQKK4 pKa = 10.4 IIDD7 pKa = 3.61 TDD9 pKa = 3.95 GVVNKK14 pKa = 9.95 LPEE17 pKa = 4.49 YY18 pKa = 7.6 GTPGYY23 pKa = 10.15 IFEE26 pKa = 4.92 LAWHH30 pKa = 7.18 DD31 pKa = 3.97 EE32 pKa = 4.01 AKK34 pKa = 10.28 KK35 pKa = 10.71 DD36 pKa = 3.74 YY37 pKa = 11.04 LVVVDD42 pKa = 5.66 AKK44 pKa = 11.22 PEE46 pKa = 4.09 VEE48 pKa = 4.5 VMNARR53 pKa = 11.84 AKK55 pKa = 10.78 GVTPYY60 pKa = 11.38 DD61 pKa = 4.31 LISTFGGIDD70 pKa = 3.38 EE71 pKa = 4.38 VTQAFASKK79 pKa = 9.73 EE80 pKa = 3.93 ASAVYY85 pKa = 10.55 ADD87 pKa = 3.68 VSGVPEE93 pKa = 4.13 FASDD97 pKa = 3.62 DD98 pKa = 4.03 VYY100 pKa = 11.82 DD101 pKa = 3.96 SVIARR106 pKa = 11.84 LKK108 pKa = 10.88 ADD110 pKa = 3.15 IAAYY114 pKa = 8.09 EE115 pKa = 4.1 ASHH118 pKa = 6.67 AKK120 pKa = 9.28 TDD122 pKa = 3.66 EE123 pKa = 4.25 SSKK126 pKa = 9.4 EE127 pKa = 3.87 TAEE130 pKa = 4.14 EE131 pKa = 3.69 VDD133 pKa = 3.45 NEE135 pKa = 4.26 VNKK138 pKa = 10.17 EE139 pKa = 4.13 VNKK142 pKa = 10.93 GEE144 pKa = 4.35 SKK146 pKa = 10.99
Molecular weight: 15.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.44
IPC2_protein 4.355
IPC_protein 4.291
Toseland 4.113
ProMoST 4.406
Dawson 4.24
Bjellqvist 4.393
Wikipedia 4.126
Rodwell 4.113
Grimsley 4.024
Solomon 4.24
Lehninger 4.19
Nozaki 4.355
DTASelect 4.52
Thurlkill 4.126
EMBOSS 4.139
Sillero 4.393
Patrickios 3.808
IPC_peptide 4.24
IPC2_peptide 4.38
IPC2.peptide.svr19 4.331
Protein with the highest isoelectric point:
>tr|A0A4P8W596|A0A4P8W596_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_550 OX=2585474 PE=4 SV=1
MM1 pKa = 6.97 MCTRR5 pKa = 11.84 PLHH8 pKa = 5.65 YY9 pKa = 10.34 FEE11 pKa = 6.71 SNLIDD16 pKa = 3.7 NEE18 pKa = 4.48 TGKK21 pKa = 10.88 KK22 pKa = 9.47 PGFITGYY29 pKa = 7.1 QVKK32 pKa = 9.99 LAGFRR37 pKa = 11.84 LFAKK41 pKa = 10.34 HH42 pKa = 6.78 GIACTEE48 pKa = 4.07 TYY50 pKa = 9.73 LAKK53 pKa = 10.19 PVEE56 pKa = 4.75 VPCGKK61 pKa = 10.37 CPEE64 pKa = 4.13 CRR66 pKa = 11.84 KK67 pKa = 9.48 ALKK70 pKa = 8.2 MQWVARR76 pKa = 11.84 AVAEE80 pKa = 4.46 LEE82 pKa = 4.46 TSKK85 pKa = 10.65 HH86 pKa = 6.01 AYY88 pKa = 9.85 FLTLTYY94 pKa = 11.12 DD95 pKa = 3.72 NDD97 pKa = 4.02 HH98 pKa = 6.75 LPSDD102 pKa = 4.21 FMCHH106 pKa = 6.35 KK107 pKa = 10.29 EE108 pKa = 4.06 HH109 pKa = 6.77 IQVFLNRR116 pKa = 11.84 FRR118 pKa = 11.84 KK119 pKa = 9.18 NNKK122 pKa = 6.74 TRR124 pKa = 11.84 YY125 pKa = 9.53 LIVGEE130 pKa = 4.29 HH131 pKa = 6.74 GEE133 pKa = 4.25 LSNRR137 pKa = 11.84 PHH139 pKa = 5.71 YY140 pKa = 10.25 HH141 pKa = 6.81 AILYY145 pKa = 7.93 TEE147 pKa = 4.4 NPIDD151 pKa = 5.47 DD152 pKa = 6.18 LIPVNKK158 pKa = 10.29 SKK160 pKa = 9.09 TLPLYY165 pKa = 10.69 EE166 pKa = 4.24 SDD168 pKa = 4.53 KK169 pKa = 10.49 VTKK172 pKa = 9.52 QWQNGIVKK180 pKa = 10.1 IGVAYY185 pKa = 8.42 PQAIAYY191 pKa = 8.97 SVGYY195 pKa = 9.85 LVSKK199 pKa = 9.74 EE200 pKa = 3.84 KK201 pKa = 9.73 KK202 pKa = 8.26 TAFKK206 pKa = 9.74 MQSQGLGYY214 pKa = 10.51 QFFSGLNRR222 pKa = 11.84 RR223 pKa = 11.84 YY224 pKa = 10.1 VLSDD228 pKa = 2.83 MHH230 pKa = 6.71 GHH232 pKa = 5.54 EE233 pKa = 5.34 MIVSLPRR240 pKa = 11.84 YY241 pKa = 8.93 LKK243 pKa = 10.54 KK244 pKa = 10.69 KK245 pKa = 9.61 YY246 pKa = 10.04 DD247 pKa = 3.69 LNFDD251 pKa = 3.86 YY252 pKa = 10.95 DD253 pKa = 3.99 DD254 pKa = 4.38 SLNSIKK260 pKa = 9.5 WHH262 pKa = 6.0 NEE264 pKa = 3.28 VVASGLKK271 pKa = 10.15 EE272 pKa = 3.49 EE273 pKa = 4.57 TYY275 pKa = 11.06 RR276 pKa = 11.84 DD277 pKa = 3.41 FKK279 pKa = 11.36 EE280 pKa = 3.84 YY281 pKa = 11.06 LEE283 pKa = 4.09 EE284 pKa = 4.93 SKK286 pKa = 10.8 LQKK289 pKa = 10.62
Molecular weight: 33.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.253
IPC2_protein 8.258
IPC_protein 8.141
Toseland 8.536
ProMoST 8.726
Dawson 8.975
Bjellqvist 8.975
Wikipedia 9.092
Rodwell 9.092
Grimsley 8.814
Solomon 9.063
Lehninger 9.033
Nozaki 9.048
DTASelect 8.829
Thurlkill 8.916
EMBOSS 9.121
Sillero 9.121
Patrickios 4.202
IPC_peptide 9.063
IPC2_peptide 7.702
IPC2.peptide.svr19 7.689
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1342
86
577
223.7
24.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.24 ± 1.407
1.043 ± 0.314
5.514 ± 0.719
6.855 ± 1.323
4.471 ± 0.558
6.408 ± 0.906
2.012 ± 0.525
5.44 ± 0.611
7.079 ± 1.471
7.899 ± 0.789
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.937 ± 0.22
5.365 ± 0.547
3.875 ± 0.765
2.906 ± 0.424
3.055 ± 0.662
7.973 ± 1.338
5.961 ± 0.644
6.334 ± 0.913
1.267 ± 0.351
5.365 ± 0.532
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here