Amino acid dipepetide frequency for Escherichia phage vB_EcoD_SU57

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.836AlaAla: 8.836 ± 1.277
0.657AlaCys: 0.657 ± 0.216
4.089AlaAsp: 4.089 ± 0.495
5.185AlaGlu: 5.185 ± 0.593
3.067AlaPhe: 3.067 ± 0.497
6.426AlaGly: 6.426 ± 0.654
1.095AlaHis: 1.095 ± 0.333
6.426AlaIle: 6.426 ± 0.706
6.134AlaLys: 6.134 ± 0.969
6.572AlaLeu: 6.572 ± 0.944
2.045AlaMet: 2.045 ± 0.431
3.87AlaAsn: 3.87 ± 0.673
2.118AlaPro: 2.118 ± 0.303
3.359AlaGln: 3.359 ± 0.659
4.601AlaArg: 4.601 ± 0.553
5.258AlaSer: 5.258 ± 0.767
4.381AlaThr: 4.381 ± 0.537
4.893AlaVal: 4.893 ± 0.631
0.949AlaTrp: 0.949 ± 0.287
2.337AlaTyr: 2.337 ± 0.33
0.0AlaXaa: 0.0 ± 0.0
Cys
0.876CysAla: 0.876 ± 0.28
0.292CysCys: 0.292 ± 0.15
0.657CysAsp: 0.657 ± 0.215
1.095CysGlu: 1.095 ± 0.271
0.438CysPhe: 0.438 ± 0.167
1.241CysGly: 1.241 ± 0.335
0.292CysHis: 0.292 ± 0.13
0.657CysIle: 0.657 ± 0.258
0.657CysLys: 0.657 ± 0.211
1.168CysLeu: 1.168 ± 0.259
0.511CysMet: 0.511 ± 0.198
0.803CysAsn: 0.803 ± 0.311
0.292CysPro: 0.292 ± 0.126
0.0CysGln: 0.0 ± 0.0
0.803CysArg: 0.803 ± 0.269
1.387CysSer: 1.387 ± 0.352
0.803CysThr: 0.803 ± 0.215
0.949CysVal: 0.949 ± 0.319
0.073CysTrp: 0.073 ± 0.076
0.584CysTyr: 0.584 ± 0.187
0.0CysXaa: 0.0 ± 0.0
Asp
5.039AspAla: 5.039 ± 0.749
0.657AspCys: 0.657 ± 0.224
4.016AspAsp: 4.016 ± 0.692
4.381AspGlu: 4.381 ± 0.623
2.702AspPhe: 2.702 ± 0.38
7.668AspGly: 7.668 ± 0.846
0.73AspHis: 0.73 ± 0.242
3.943AspIle: 3.943 ± 0.487
3.87AspLys: 3.87 ± 0.449
3.943AspLeu: 3.943 ± 0.538
1.387AspMet: 1.387 ± 0.356
3.286AspAsn: 3.286 ± 0.493
1.753AspPro: 1.753 ± 0.363
1.241AspGln: 1.241 ± 0.295
1.607AspArg: 1.607 ± 0.486
3.797AspSer: 3.797 ± 0.618
3.067AspThr: 3.067 ± 0.506
3.87AspVal: 3.87 ± 0.478
0.949AspTrp: 0.949 ± 0.25
3.213AspTyr: 3.213 ± 0.56
0.0AspXaa: 0.0 ± 0.0
Glu
4.747GluAla: 4.747 ± 0.627
0.949GluCys: 0.949 ± 0.296
3.067GluAsp: 3.067 ± 0.567
4.016GluGlu: 4.016 ± 0.693
3.505GluPhe: 3.505 ± 0.454
3.578GluGly: 3.578 ± 0.433
0.949GluHis: 0.949 ± 0.252
5.404GluIle: 5.404 ± 0.7
3.87GluLys: 3.87 ± 0.686
6.207GluLeu: 6.207 ± 0.692
2.483GluMet: 2.483 ± 0.511
3.213GluAsn: 3.213 ± 0.525
2.191GluPro: 2.191 ± 0.393
2.775GluGln: 2.775 ± 0.613
2.629GluArg: 2.629 ± 0.479
4.966GluSer: 4.966 ± 0.631
2.994GluThr: 2.994 ± 0.434
5.623GluVal: 5.623 ± 0.725
0.803GluTrp: 0.803 ± 0.299
2.629GluTyr: 2.629 ± 0.455
0.0GluXaa: 0.0 ± 0.0
Phe
2.045PheAla: 2.045 ± 0.464
0.949PheCys: 0.949 ± 0.331
3.578PheAsp: 3.578 ± 0.498
2.994PheGlu: 2.994 ± 0.582
1.314PhePhe: 1.314 ± 0.306
3.213PheGly: 3.213 ± 0.508
1.022PheHis: 1.022 ± 0.317
3.14PheIle: 3.14 ± 0.477
3.067PheLys: 3.067 ± 0.526
1.826PheLeu: 1.826 ± 0.413
0.876PheMet: 0.876 ± 0.253
2.337PheAsn: 2.337 ± 0.318
1.241PhePro: 1.241 ± 0.268
1.899PheGln: 1.899 ± 0.36
2.045PheArg: 2.045 ± 0.552
2.41PheSer: 2.41 ± 0.448
2.41PheThr: 2.41 ± 0.346
2.848PheVal: 2.848 ± 0.425
0.365PheTrp: 0.365 ± 0.128
1.241PheTyr: 1.241 ± 0.277
0.0PheXaa: 0.0 ± 0.0
Gly
4.674GlyAla: 4.674 ± 0.869
1.826GlyCys: 1.826 ± 0.416
4.528GlyAsp: 4.528 ± 0.496
4.674GlyGlu: 4.674 ± 0.599
2.848GlyPhe: 2.848 ± 0.487
6.645GlyGly: 6.645 ± 1.213
0.584GlyHis: 0.584 ± 0.284
5.842GlyIle: 5.842 ± 0.518
5.55GlyLys: 5.55 ± 0.783
6.426GlyLeu: 6.426 ± 0.636
2.264GlyMet: 2.264 ± 0.488
3.505GlyAsn: 3.505 ± 0.494
0.657GlyPro: 0.657 ± 0.22
1.972GlyGln: 1.972 ± 0.517
3.578GlyArg: 3.578 ± 0.457
5.185GlySer: 5.185 ± 0.635
4.162GlyThr: 4.162 ± 0.783
6.061GlyVal: 6.061 ± 0.689
1.168GlyTrp: 1.168 ± 0.285
4.308GlyTyr: 4.308 ± 0.598
0.0GlyXaa: 0.0 ± 0.0
His
1.168HisAla: 1.168 ± 0.338
0.292HisCys: 0.292 ± 0.147
0.803HisAsp: 0.803 ± 0.214
0.949HisGlu: 0.949 ± 0.29
0.876HisPhe: 0.876 ± 0.303
1.022HisGly: 1.022 ± 0.272
0.219HisHis: 0.219 ± 0.126
1.168HisIle: 1.168 ± 0.383
1.534HisLys: 1.534 ± 0.401
1.387HisLeu: 1.387 ± 0.319
0.292HisMet: 0.292 ± 0.157
0.292HisAsn: 0.292 ± 0.138
0.146HisPro: 0.146 ± 0.1
0.365HisGln: 0.365 ± 0.143
0.803HisArg: 0.803 ± 0.206
0.803HisSer: 0.803 ± 0.285
0.73HisThr: 0.73 ± 0.211
0.949HisVal: 0.949 ± 0.243
0.073HisTrp: 0.073 ± 0.064
0.438HisTyr: 0.438 ± 0.186
0.0HisXaa: 0.0 ± 0.0
Ile
6.134IleAla: 6.134 ± 0.874
0.511IleCys: 0.511 ± 0.217
6.426IleAsp: 6.426 ± 0.524
3.359IleGlu: 3.359 ± 0.425
2.191IlePhe: 2.191 ± 0.302
4.235IleGly: 4.235 ± 0.634
0.949IleHis: 0.949 ± 0.259
4.162IleIle: 4.162 ± 0.608
5.477IleLys: 5.477 ± 0.655
3.651IleLeu: 3.651 ± 0.501
1.753IleMet: 1.753 ± 0.377
4.235IleAsn: 4.235 ± 0.59
3.067IlePro: 3.067 ± 0.492
2.118IleGln: 2.118 ± 0.493
3.286IleArg: 3.286 ± 0.432
5.039IleSer: 5.039 ± 0.503
4.601IleThr: 4.601 ± 0.61
3.432IleVal: 3.432 ± 0.409
1.095IleTrp: 1.095 ± 0.248
3.578IleTyr: 3.578 ± 0.57
0.0IleXaa: 0.0 ± 0.0
Lys
6.718LysAla: 6.718 ± 0.792
0.511LysCys: 0.511 ± 0.21
4.308LysAsp: 4.308 ± 0.544
5.185LysGlu: 5.185 ± 0.779
3.067LysPhe: 3.067 ± 0.513
3.14LysGly: 3.14 ± 0.49
1.241LysHis: 1.241 ± 0.309
3.724LysIle: 3.724 ± 0.435
3.943LysLys: 3.943 ± 0.519
4.528LysLeu: 4.528 ± 0.645
2.994LysMet: 2.994 ± 0.498
2.921LysAsn: 2.921 ± 0.425
1.753LysPro: 1.753 ± 0.386
2.702LysGln: 2.702 ± 0.383
2.483LysArg: 2.483 ± 0.501
4.455LysSer: 4.455 ± 0.632
3.651LysThr: 3.651 ± 0.541
4.966LysVal: 4.966 ± 0.636
0.803LysTrp: 0.803 ± 0.257
3.286LysTyr: 3.286 ± 0.548
0.0LysXaa: 0.0 ± 0.0
Leu
6.28LeuAla: 6.28 ± 0.722
1.022LeuCys: 1.022 ± 0.257
4.528LeuAsp: 4.528 ± 0.587
4.381LeuGlu: 4.381 ± 0.527
2.337LeuPhe: 2.337 ± 0.31
4.455LeuGly: 4.455 ± 0.63
0.876LeuHis: 0.876 ± 0.263
4.893LeuIle: 4.893 ± 0.731
4.016LeuLys: 4.016 ± 0.449
4.381LeuLeu: 4.381 ± 0.509
1.753LeuMet: 1.753 ± 0.451
3.14LeuAsn: 3.14 ± 0.476
2.848LeuPro: 2.848 ± 0.415
2.629LeuGln: 2.629 ± 0.684
3.797LeuArg: 3.797 ± 0.542
5.988LeuSer: 5.988 ± 0.814
4.235LeuThr: 4.235 ± 0.729
5.404LeuVal: 5.404 ± 0.498
0.438LeuTrp: 0.438 ± 0.247
1.826LeuTyr: 1.826 ± 0.426
0.0LeuXaa: 0.0 ± 0.0
Met
3.286MetAla: 3.286 ± 0.46
0.292MetCys: 0.292 ± 0.147
1.095MetAsp: 1.095 ± 0.356
1.534MetGlu: 1.534 ± 0.362
1.241MetPhe: 1.241 ± 0.281
1.095MetGly: 1.095 ± 0.297
0.365MetHis: 0.365 ± 0.168
2.848MetIle: 2.848 ± 0.449
2.045MetLys: 2.045 ± 0.326
1.972MetLeu: 1.972 ± 0.522
0.584MetMet: 0.584 ± 0.256
1.68MetAsn: 1.68 ± 0.396
0.219MetPro: 0.219 ± 0.131
0.73MetGln: 0.73 ± 0.204
1.387MetArg: 1.387 ± 0.299
1.534MetSer: 1.534 ± 0.307
2.191MetThr: 2.191 ± 0.407
1.387MetVal: 1.387 ± 0.3
0.292MetTrp: 0.292 ± 0.154
0.584MetTyr: 0.584 ± 0.197
0.0MetXaa: 0.0 ± 0.0
Asn
3.432AsnAla: 3.432 ± 0.52
0.949AsnCys: 0.949 ± 0.291
2.702AsnAsp: 2.702 ± 0.418
4.308AsnGlu: 4.308 ± 0.6
1.972AsnPhe: 1.972 ± 0.33
6.061AsnGly: 6.061 ± 1.128
0.73AsnHis: 0.73 ± 0.272
2.848AsnIle: 2.848 ± 0.345
2.702AsnLys: 2.702 ± 0.475
3.286AsnLeu: 3.286 ± 0.756
1.241AsnMet: 1.241 ± 0.267
3.14AsnAsn: 3.14 ± 0.508
1.68AsnPro: 1.68 ± 0.284
2.118AsnGln: 2.118 ± 0.472
2.41AsnArg: 2.41 ± 0.286
3.578AsnSer: 3.578 ± 0.645
2.191AsnThr: 2.191 ± 0.354
3.578AsnVal: 3.578 ± 0.491
0.73AsnTrp: 0.73 ± 0.196
1.826AsnTyr: 1.826 ± 0.313
0.0AsnXaa: 0.0 ± 0.0
Pro
3.14ProAla: 3.14 ± 0.431
0.365ProCys: 0.365 ± 0.2
2.045ProAsp: 2.045 ± 0.57
3.067ProGlu: 3.067 ± 0.459
1.753ProPhe: 1.753 ± 0.347
1.68ProGly: 1.68 ± 0.361
0.438ProHis: 0.438 ± 0.177
1.607ProIle: 1.607 ± 0.293
1.46ProLys: 1.46 ± 0.381
1.972ProLeu: 1.972 ± 0.358
0.73ProMet: 0.73 ± 0.217
1.46ProAsn: 1.46 ± 0.343
0.657ProPro: 0.657 ± 0.205
1.022ProGln: 1.022 ± 0.294
1.314ProArg: 1.314 ± 0.335
1.095ProSer: 1.095 ± 0.292
1.387ProThr: 1.387 ± 0.319
3.286ProVal: 3.286 ± 0.578
0.365ProTrp: 0.365 ± 0.187
1.387ProTyr: 1.387 ± 0.292
0.0ProXaa: 0.0 ± 0.0
Gln
3.943GlnAla: 3.943 ± 0.853
0.365GlnCys: 0.365 ± 0.155
1.607GlnAsp: 1.607 ± 0.306
2.264GlnGlu: 2.264 ± 0.49
1.241GlnPhe: 1.241 ± 0.265
2.629GlnGly: 2.629 ± 0.552
0.511GlnHis: 0.511 ± 0.213
3.286GlnIle: 3.286 ± 0.606
2.118GlnLys: 2.118 ± 0.426
2.848GlnLeu: 2.848 ± 0.616
0.73GlnMet: 0.73 ± 0.264
1.826GlnAsn: 1.826 ± 0.461
1.022GlnPro: 1.022 ± 0.293
3.067GlnGln: 3.067 ± 1.523
1.314GlnArg: 1.314 ± 0.326
2.775GlnSer: 2.775 ± 0.4
1.826GlnThr: 1.826 ± 0.35
2.264GlnVal: 2.264 ± 0.354
0.511GlnTrp: 0.511 ± 0.176
1.314GlnTyr: 1.314 ± 0.322
0.0GlnXaa: 0.0 ± 0.0
Arg
3.578ArgAla: 3.578 ± 0.494
0.876ArgCys: 0.876 ± 0.345
1.68ArgAsp: 1.68 ± 0.337
3.286ArgGlu: 3.286 ± 0.399
2.191ArgPhe: 2.191 ± 0.338
2.921ArgGly: 2.921 ± 0.473
0.803ArgHis: 0.803 ± 0.253
3.578ArgIle: 3.578 ± 0.452
4.162ArgLys: 4.162 ± 0.629
3.797ArgLeu: 3.797 ± 0.501
1.168ArgMet: 1.168 ± 0.36
2.41ArgAsn: 2.41 ± 0.472
1.534ArgPro: 1.534 ± 0.336
1.168ArgGln: 1.168 ± 0.332
2.191ArgArg: 2.191 ± 0.393
2.702ArgSer: 2.702 ± 0.496
2.118ArgThr: 2.118 ± 0.435
3.505ArgVal: 3.505 ± 0.479
0.511ArgTrp: 0.511 ± 0.172
1.607ArgTyr: 1.607 ± 0.318
0.0ArgXaa: 0.0 ± 0.0
Ser
5.623SerAla: 5.623 ± 0.735
0.584SerCys: 0.584 ± 0.195
5.039SerAsp: 5.039 ± 0.478
5.331SerGlu: 5.331 ± 0.566
2.337SerPhe: 2.337 ± 0.402
6.572SerGly: 6.572 ± 0.903
1.387SerHis: 1.387 ± 0.342
3.724SerIle: 3.724 ± 0.423
4.162SerLys: 4.162 ± 0.695
4.528SerLeu: 4.528 ± 0.528
1.534SerMet: 1.534 ± 0.288
3.286SerAsn: 3.286 ± 0.552
2.41SerPro: 2.41 ± 0.386
2.483SerGln: 2.483 ± 0.493
3.067SerArg: 3.067 ± 0.476
5.769SerSer: 5.769 ± 1.05
3.797SerThr: 3.797 ± 0.704
5.404SerVal: 5.404 ± 0.682
0.657SerTrp: 0.657 ± 0.194
2.483SerTyr: 2.483 ± 0.417
0.0SerXaa: 0.0 ± 0.0
Thr
4.308ThrAla: 4.308 ± 0.62
0.876ThrCys: 0.876 ± 0.236
2.483ThrAsp: 2.483 ± 0.39
2.264ThrGlu: 2.264 ± 0.395
2.483ThrPhe: 2.483 ± 0.453
5.623ThrGly: 5.623 ± 1.002
0.365ThrHis: 0.365 ± 0.136
4.016ThrIle: 4.016 ± 0.466
2.994ThrLys: 2.994 ± 0.533
3.432ThrLeu: 3.432 ± 0.435
1.022ThrMet: 1.022 ± 0.261
3.286ThrAsn: 3.286 ± 0.555
2.337ThrPro: 2.337 ± 0.378
2.775ThrGln: 2.775 ± 0.534
2.045ThrArg: 2.045 ± 0.351
3.797ThrSer: 3.797 ± 0.545
2.775ThrThr: 2.775 ± 0.538
4.381ThrVal: 4.381 ± 0.551
0.73ThrTrp: 0.73 ± 0.223
2.702ThrTyr: 2.702 ± 0.418
0.0ThrXaa: 0.0 ± 0.0
Val
5.112ValAla: 5.112 ± 0.561
0.876ValCys: 0.876 ± 0.275
4.528ValAsp: 4.528 ± 0.509
4.82ValGlu: 4.82 ± 0.682
3.067ValPhe: 3.067 ± 0.407
4.674ValGly: 4.674 ± 0.641
1.095ValHis: 1.095 ± 0.282
4.308ValIle: 4.308 ± 0.516
4.966ValLys: 4.966 ± 0.647
4.016ValLeu: 4.016 ± 0.445
1.534ValMet: 1.534 ± 0.316
4.601ValAsn: 4.601 ± 0.44
2.337ValPro: 2.337 ± 0.412
2.702ValGln: 2.702 ± 0.528
3.651ValArg: 3.651 ± 0.467
6.061ValSer: 6.061 ± 0.838
4.381ValThr: 4.381 ± 0.448
5.477ValVal: 5.477 ± 0.776
0.803ValTrp: 0.803 ± 0.226
2.702ValTyr: 2.702 ± 0.495
0.0ValXaa: 0.0 ± 0.0
Trp
0.73TrpAla: 0.73 ± 0.166
0.219TrpCys: 0.219 ± 0.149
0.949TrpAsp: 0.949 ± 0.242
0.657TrpGlu: 0.657 ± 0.245
0.73TrpPhe: 0.73 ± 0.232
0.803TrpGly: 0.803 ± 0.191
0.219TrpHis: 0.219 ± 0.123
1.022TrpIle: 1.022 ± 0.319
1.241TrpLys: 1.241 ± 0.307
0.949TrpLeu: 0.949 ± 0.246
0.365TrpMet: 0.365 ± 0.163
0.219TrpAsn: 0.219 ± 0.113
0.365TrpPro: 0.365 ± 0.187
0.511TrpGln: 0.511 ± 0.171
0.803TrpArg: 0.803 ± 0.22
0.803TrpSer: 0.803 ± 0.322
0.292TrpThr: 0.292 ± 0.133
0.73TrpVal: 0.73 ± 0.203
0.073TrpTrp: 0.073 ± 0.076
0.219TrpTyr: 0.219 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.629TyrAla: 2.629 ± 0.437
0.511TyrCys: 0.511 ± 0.173
2.994TyrAsp: 2.994 ± 0.559
2.848TyrGlu: 2.848 ± 0.466
1.46TyrPhe: 1.46 ± 0.369
2.775TyrGly: 2.775 ± 0.367
0.511TyrHis: 0.511 ± 0.198
2.702TyrIle: 2.702 ± 0.438
2.41TyrLys: 2.41 ± 0.41
2.337TyrLeu: 2.337 ± 0.414
1.022TyrMet: 1.022 ± 0.283
1.972TyrAsn: 1.972 ± 0.314
1.607TyrPro: 1.607 ± 0.452
1.826TyrGln: 1.826 ± 0.396
1.972TyrArg: 1.972 ± 0.398
2.775TyrSer: 2.775 ± 0.477
2.702TyrThr: 2.702 ± 0.391
2.702TyrVal: 2.702 ± 0.456
0.511TyrTrp: 0.511 ± 0.167
1.826TyrTyr: 1.826 ± 0.375
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (13695 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski