Natrialba magadii (strain ATCC 43099 / DSM 3394 / CCM 3739 / CIP 104546 / IAM 13178 / JCM 8861 / NBRC 102185 / NCIMB 2190 / MS3) (Natronobacterium magadii)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Natrialba; Natrialba magadii

Average proteome isoelectric point is 4.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4203 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3SWB9|D3SWB9_NATMM Uncharacterized protein OS=Natrialba magadii (strain ATCC 43099 / DSM 3394 / CCM 3739 / CIP 104546 / IAM 13178 / JCM 8861 / NBRC 102185 / NCIMB 2190 / MS3) OX=547559 GN=Nmag_0113 PE=4 SV=1
MM1 pKa = 6.2VHH3 pKa = 6.53RR4 pKa = 11.84RR5 pKa = 11.84NVLKK9 pKa = 10.63GAGAISVAGMAGCLGGDD26 pKa = 3.52DD27 pKa = 4.58GEE29 pKa = 4.45YY30 pKa = 9.81TRR32 pKa = 11.84PVEE35 pKa = 4.93IDD37 pKa = 3.27FDD39 pKa = 3.82DD40 pKa = 4.27WPPEE44 pKa = 3.88EE45 pKa = 4.98YY46 pKa = 10.69GDD48 pKa = 5.77SLHH51 pKa = 6.28AWNWYY56 pKa = 9.36GEE58 pKa = 4.15WNEE61 pKa = 3.73WGAEE65 pKa = 4.04NFAEE69 pKa = 4.64EE70 pKa = 4.63YY71 pKa = 10.61DD72 pKa = 3.65LSSYY76 pKa = 7.42EE77 pKa = 4.18TEE79 pKa = 4.58VYY81 pKa = 7.94ATPSDD86 pKa = 3.43WFTNIQANPDD96 pKa = 3.08NHH98 pKa = 7.04GIDD101 pKa = 3.44HH102 pKa = 6.61VGLSTEE108 pKa = 4.04WIHH111 pKa = 7.22RR112 pKa = 11.84VNQEE116 pKa = 4.11DD117 pKa = 3.92ALEE120 pKa = 4.84PIPVDD125 pKa = 3.3EE126 pKa = 5.14LPNVDD131 pKa = 4.61VADD134 pKa = 3.9QYY136 pKa = 11.4MDD138 pKa = 3.0LHH140 pKa = 6.85RR141 pKa = 11.84EE142 pKa = 4.04HH143 pKa = 6.97FGSDD147 pKa = 3.18DD148 pKa = 3.76GVGGVYY154 pKa = 10.23AVPHH158 pKa = 6.89AIVLGPVVVYY168 pKa = 7.81NTNEE172 pKa = 3.77IQDD175 pKa = 4.05PPEE178 pKa = 5.91SIDD181 pKa = 3.47ILWDD185 pKa = 3.13EE186 pKa = 4.79EE187 pKa = 4.4YY188 pKa = 11.2ADD190 pKa = 5.12EE191 pKa = 5.07ISMMAHH197 pKa = 6.99LSGFLCEE204 pKa = 5.45AGALYY209 pKa = 9.84TGQDD213 pKa = 3.8PNDD216 pKa = 4.06PDD218 pKa = 3.78DD219 pKa = 4.35FEE221 pKa = 5.39EE222 pKa = 4.09IRR224 pKa = 11.84EE225 pKa = 4.06VLEE228 pKa = 3.84QQRR231 pKa = 11.84DD232 pKa = 3.69LVFNYY237 pKa = 10.37ADD239 pKa = 3.37EE240 pKa = 5.75HH241 pKa = 5.24EE242 pKa = 4.55TQMQLLMSGDD252 pKa = 3.81ASLGTHH258 pKa = 5.42TDD260 pKa = 2.97GRR262 pKa = 11.84AFRR265 pKa = 11.84AMYY268 pKa = 10.49NHH270 pKa = 6.9GADD273 pKa = 3.56VDD275 pKa = 3.7WFIPEE280 pKa = 4.11EE281 pKa = 4.33GTVVGPNEE289 pKa = 3.87VAIPTGGPNPITSTMYY305 pKa = 10.15TDD307 pKa = 4.21YY308 pKa = 11.41LFTDD312 pKa = 3.47EE313 pKa = 5.48GMEE316 pKa = 4.09KK317 pKa = 10.24LVEE320 pKa = 4.28TTVYY324 pKa = 9.89RR325 pKa = 11.84PPMEE329 pKa = 4.86NEE331 pKa = 3.57AFTDD335 pKa = 4.29GEE337 pKa = 4.25FGDD340 pKa = 4.47TIRR343 pKa = 11.84DD344 pKa = 3.27KK345 pKa = 11.04WDD347 pKa = 3.99DD348 pKa = 3.44EE349 pKa = 4.38WEE351 pKa = 4.28KK352 pKa = 11.32EE353 pKa = 3.63GDD355 pKa = 3.64AEE357 pKa = 5.75DD358 pKa = 5.56FIDD361 pKa = 5.32DD362 pKa = 4.26LVITEE367 pKa = 4.85DD368 pKa = 3.65EE369 pKa = 4.5LEE371 pKa = 4.14RR372 pKa = 11.84SYY374 pKa = 11.43DD375 pKa = 2.96AWPRR379 pKa = 11.84SDD381 pKa = 5.59DD382 pKa = 3.7VIEE385 pKa = 5.13RR386 pKa = 11.84YY387 pKa = 10.38DD388 pKa = 4.11EE389 pKa = 3.69IWTEE393 pKa = 3.71ITTGG397 pKa = 3.27

Molecular weight: 45.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3SYS5|D3SYS5_NATMM DUF3054 family protein OS=Natrialba magadii (strain ATCC 43099 / DSM 3394 / CCM 3739 / CIP 104546 / IAM 13178 / JCM 8861 / NBRC 102185 / NCIMB 2190 / MS3) OX=547559 GN=Nmag_0600 PE=4 SV=1
MM1 pKa = 7.28ATLTKK6 pKa = 10.14SVPKK10 pKa = 10.38RR11 pKa = 11.84PIGRR15 pKa = 11.84NPRR18 pKa = 11.84VHH20 pKa = 6.63HH21 pKa = 5.87LLGRR25 pKa = 11.84FCLGRR30 pKa = 11.84PEE32 pKa = 4.32GGTRR36 pKa = 11.84VRR38 pKa = 11.84RR39 pKa = 11.84DD40 pKa = 3.62CDD42 pKa = 2.87RR43 pKa = 11.84LSQVRR48 pKa = 11.84GRR50 pKa = 11.84VDD52 pKa = 2.67GWLFGNQSVCDD63 pKa = 3.47RR64 pKa = 11.84VQVQEE69 pKa = 4.23PVPVSEE75 pKa = 4.63VCLKK79 pKa = 9.66TALVCLLTGGRR90 pKa = 11.84NTHH93 pKa = 6.07SARR96 pKa = 11.84DD97 pKa = 3.67PAGVTVFAVCSRR109 pKa = 11.84ADD111 pKa = 3.97GPLAWAAGSTIPRR124 pKa = 11.84SVLSRR129 pKa = 11.84GSHH132 pKa = 5.37SLCSLQTALAPNRR145 pKa = 11.84ARR147 pKa = 11.84CRR149 pKa = 11.84PRR151 pKa = 11.84LLRR154 pKa = 11.84TTRR157 pKa = 11.84SSWDD161 pKa = 3.21GCAVSYY167 pKa = 11.09SDD169 pKa = 5.25YY170 pKa = 11.39YY171 pKa = 11.77SNNCNN176 pKa = 3.41

Molecular weight: 19.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4203

0

4203

1227618

30

1773

292.1

31.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.293 ± 0.045

0.756 ± 0.012

8.786 ± 0.057

9.132 ± 0.064

3.201 ± 0.025

8.14 ± 0.035

2.136 ± 0.019

4.553 ± 0.033

1.774 ± 0.026

8.686 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.736 ± 0.016

2.502 ± 0.022

4.567 ± 0.026

2.818 ± 0.021

5.952 ± 0.037

5.966 ± 0.033

6.874 ± 0.034

8.314 ± 0.037

1.127 ± 0.014

2.688 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski