Pediococcus phage cIP1
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8FV15|G8FV15_9CAUD Uncharacterized protein OS=Pediococcus phage cIP1 OX=2681621 GN=clP1_032 PE=4 SV=1
MM1 pKa = 6.09 MTLYY5 pKa = 10.86 NVGDD9 pKa = 3.78 TVRR12 pKa = 11.84 LYY14 pKa = 10.62 NGTVGVVDD22 pKa = 3.84 VVTSGGALLIRR33 pKa = 11.84 GQYY36 pKa = 7.71 VQPDD40 pKa = 3.65 DD41 pKa = 3.75 VFNVCRR47 pKa = 11.84 KK48 pKa = 9.49 LKK50 pKa = 10.4 PGDD53 pKa = 3.51 VVTFKK58 pKa = 10.59 PVYY61 pKa = 9.36 EE62 pKa = 4.32 LRR64 pKa = 11.84 QCPLWGQYY72 pKa = 11.08 GDD74 pKa = 5.32 DD75 pKa = 3.9 EE76 pKa = 6.41 LDD78 pKa = 3.44 TVFGIRR84 pKa = 11.84 TADD87 pKa = 3.27 IPEE90 pKa = 4.32 KK91 pKa = 10.4 ATVDD95 pKa = 3.5 VCHH98 pKa = 7.21 CYY100 pKa = 10.04 PGSDD104 pKa = 3.44 DD105 pKa = 3.71 VFTIEE110 pKa = 4.15 DD111 pKa = 3.76 SEE113 pKa = 4.84 YY114 pKa = 11.09 SFLSSMIEE122 pKa = 4.2 RR123 pKa = 11.84 IDD125 pKa = 3.28
Molecular weight: 13.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.884
IPC2_protein 4.164
IPC_protein 4.126
Toseland 3.91
ProMoST 4.215
Dawson 4.113
Bjellqvist 4.317
Wikipedia 4.062
Rodwell 3.948
Grimsley 3.821
Solomon 4.113
Lehninger 4.062
Nozaki 4.228
DTASelect 4.495
Thurlkill 3.961
EMBOSS 4.075
Sillero 4.24
Patrickios 1.977
IPC_peptide 4.101
IPC2_peptide 4.228
IPC2.peptide.svr19 4.143
Protein with the highest isoelectric point:
>tr|G8FV22|G8FV22_9CAUD Uncharacterized protein OS=Pediococcus phage cIP1 OX=2681621 GN=clP1_039 PE=4 SV=1
MM1 pKa = 7.34 SFAPGKK7 pKa = 10.9 YY8 pKa = 8.78 EE9 pKa = 3.8 VSDD12 pKa = 3.61 LGRR15 pKa = 11.84 VRR17 pKa = 11.84 RR18 pKa = 11.84 AAWVGSGGKK27 pKa = 8.86 KK28 pKa = 9.66 HH29 pKa = 6.62 PARR32 pKa = 11.84 IVKK35 pKa = 9.4 QWDD38 pKa = 3.11 NGQRR42 pKa = 11.84 AANVTFYY49 pKa = 11.29 DD50 pKa = 3.52 DD51 pKa = 3.34 HH52 pKa = 6.4 TKK54 pKa = 10.12 VNRR57 pKa = 11.84 RR58 pKa = 11.84 VGMLVAEE65 pKa = 5.09 AFMADD70 pKa = 4.13 QPGKK74 pKa = 9.68 WVLHH78 pKa = 6.42 HH79 pKa = 7.71 DD80 pKa = 3.54 GDD82 pKa = 3.68 NWNNRR87 pKa = 11.84 LSNLFKK93 pKa = 10.61 SDD95 pKa = 3.14 NRR97 pKa = 11.84 HH98 pKa = 5.07 NPEE101 pKa = 2.99 ARR103 pKa = 11.84 ARR105 pKa = 11.84 HH106 pKa = 5.54 LFVGGKK112 pKa = 8.92 HH113 pKa = 5.61 FKK115 pKa = 9.85 SAHH118 pKa = 5.52 DD119 pKa = 3.36 AARR122 pKa = 11.84 YY123 pKa = 9.48 LRR125 pKa = 11.84 VSDD128 pKa = 3.44 TTIRR132 pKa = 11.84 TAATAGKK139 pKa = 9.67 KK140 pKa = 10.12 INDD143 pKa = 3.92 KK144 pKa = 10.81 KK145 pKa = 11.24 VIWEE149 pKa = 4.05 KK150 pKa = 11.28
Molecular weight: 17.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.212
IPC2_protein 9.56
IPC_protein 9.78
Toseland 10.657
ProMoST 10.218
Dawson 10.73
Bjellqvist 10.35
Wikipedia 10.862
Rodwell 11.184
Grimsley 10.76
Solomon 10.789
Lehninger 10.774
Nozaki 10.613
DTASelect 10.35
Thurlkill 10.628
EMBOSS 11.038
Sillero 10.643
Patrickios 10.921
IPC_peptide 10.804
IPC2_peptide 8.77
IPC2.peptide.svr19 8.771
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
0
57
11576
42
1084
203.1
22.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.166 ± 0.666
0.501 ± 0.107
7.11 ± 0.388
5.425 ± 0.418
3.732 ± 0.201
7.498 ± 0.397
1.693 ± 0.146
6.133 ± 0.284
6.453 ± 0.263
7.058 ± 0.229
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.222 ± 0.263
5.382 ± 0.215
3.119 ± 0.215
3.17 ± 0.177
4.829 ± 0.408
6.056 ± 0.389
6.323 ± 0.25
7.481 ± 0.303
1.132 ± 0.118
4.518 ± 0.368
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here