Brevibacterium casei S18

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Brevibacteriaceae; Brevibacterium; Brevibacterium casei

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3216 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9AJ02|K9AJ02_9MICO Putative phosphatase/phosphohexomutase OS=Brevibacterium casei S18 OX=1229781 GN=C272_08712 PE=4 SV=1
MM1 pKa = 7.48IVLDD5 pKa = 4.64APDD8 pKa = 4.1AGEE11 pKa = 3.84LARR14 pKa = 11.84FYY16 pKa = 11.58GDD18 pKa = 3.51LLDD21 pKa = 3.63WQVRR25 pKa = 11.84VDD27 pKa = 6.18DD28 pKa = 5.06DD29 pKa = 4.29GTWAEE34 pKa = 4.3ITSDD38 pKa = 3.6SWPPICFQQVEE49 pKa = 4.92DD50 pKa = 3.77YY51 pKa = 10.26HH52 pKa = 8.04APSWPSQEE60 pKa = 5.46HH61 pKa = 5.68PQQMHH66 pKa = 6.67IDD68 pKa = 3.87VMVDD72 pKa = 3.32DD73 pKa = 5.58LDD75 pKa = 3.9AAEE78 pKa = 4.44AQVIEE83 pKa = 4.57LGAGKK88 pKa = 10.02AAVQPGEE95 pKa = 4.17TFRR98 pKa = 11.84VFLDD102 pKa = 3.71PAGHH106 pKa = 6.91PFCLCVSS113 pKa = 4.09

Molecular weight: 12.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9AR41|K9AR41_9MICO Error-prone DNA polymerase OS=Brevibacterium casei S18 OX=1229781 GN=dnaE2 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.99RR13 pKa = 11.84AKK15 pKa = 9.77KK16 pKa = 9.51HH17 pKa = 4.17GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.72GRR40 pKa = 11.84SEE42 pKa = 3.94VSAA45 pKa = 4.23

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3216

0

3216

1086725

36

1709

337.9

36.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.773 ± 0.053

0.565 ± 0.011

6.553 ± 0.04

6.17 ± 0.043

3.234 ± 0.026

9.023 ± 0.044

1.97 ± 0.017

4.885 ± 0.033

2.329 ± 0.037

9.811 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.913 ± 0.016

2.016 ± 0.022

5.357 ± 0.033

2.595 ± 0.02

6.826 ± 0.043

6.032 ± 0.034

6.34 ± 0.029

8.38 ± 0.035

1.34 ± 0.016

1.888 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski