Halobacillus sp. BBL2006

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Halobacillus; unclassified Halobacillus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4119 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B0CY90|A0A0B0CY90_9BACI HTH lysR-type domain-containing protein OS=Halobacillus sp. BBL2006 OX=1543706 GN=LD39_16420 PE=4 SV=1
MM1 pKa = 7.58AKK3 pKa = 9.87ILICFASMSGNTEE16 pKa = 3.93DD17 pKa = 5.51LADD20 pKa = 5.49LIQTYY25 pKa = 10.34LEE27 pKa = 4.42EE28 pKa = 5.47DD29 pKa = 3.41GHH31 pKa = 5.03TVFMEE36 pKa = 4.19EE37 pKa = 4.37MDD39 pKa = 5.02EE40 pKa = 4.02IDD42 pKa = 2.96PSYY45 pKa = 10.39FHH47 pKa = 7.39EE48 pKa = 4.56YY49 pKa = 9.97DD50 pKa = 3.16GSIIGSYY57 pKa = 7.5TWNDD61 pKa = 2.43GDD63 pKa = 4.74LPYY66 pKa = 10.43EE67 pKa = 4.52AEE69 pKa = 4.22DD70 pKa = 4.64FYY72 pKa = 11.77DD73 pKa = 3.9GLEE76 pKa = 4.34DD77 pKa = 3.98EE78 pKa = 5.15DD79 pKa = 4.53LKK81 pKa = 11.45GVPVCVFGSGDD92 pKa = 3.0TAYY95 pKa = 9.91PKK97 pKa = 10.52FCEE100 pKa = 3.97AVNIFEE106 pKa = 4.74KK107 pKa = 11.22GLMNRR112 pKa = 11.84GATLMQEE119 pKa = 4.21GLKK122 pKa = 9.96IEE124 pKa = 4.21MDD126 pKa = 3.78PDD128 pKa = 3.8GEE130 pKa = 4.03EE131 pKa = 4.65DD132 pKa = 3.8VVNCRR137 pKa = 11.84RR138 pKa = 11.84FVLNVSKK145 pKa = 11.0YY146 pKa = 7.71LTEE149 pKa = 4.1VNII152 pKa = 4.29

Molecular weight: 17.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B0D2R9|A0A0B0D2R9_9BACI Flagellar motor protein MotS (Fragment) OS=Halobacillus sp. BBL2006 OX=1543706 GN=LD39_11765 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.48NGRR28 pKa = 11.84KK29 pKa = 8.82VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.59GRR39 pKa = 11.84KK40 pKa = 8.44VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4119

0

4119

1069533

26

1526

259.7

29.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.849 ± 0.037

0.624 ± 0.012

5.464 ± 0.037

7.954 ± 0.049

4.527 ± 0.033

7.0 ± 0.037

2.251 ± 0.02

7.196 ± 0.038

6.516 ± 0.032

9.529 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.922 ± 0.019

4.12 ± 0.024

3.754 ± 0.021

3.949 ± 0.028

4.195 ± 0.027

6.133 ± 0.031

5.277 ± 0.024

7.121 ± 0.032

1.092 ± 0.015

3.527 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski