Wongabel hapavirus
Average proteome isoelectric point is 5.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B2X7D3|B2X7D3_9RHAB Polymerase-associated protein OS=Wongabel hapavirus OX=1972626 GN=P PE=4 SV=1
MM1 pKa = 7.34 MLFKK5 pKa = 11.09 LNLISNIPFVIYY17 pKa = 9.75 EE18 pKa = 4.08 IQEE21 pKa = 3.8 LTGFIIYY28 pKa = 9.96 IEE30 pKa = 4.28 FPVEE34 pKa = 3.86 CEE36 pKa = 3.53 NMLFYY41 pKa = 11.19 GLQIAGYY48 pKa = 6.51 TT49 pKa = 3.55
Molecular weight: 5.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.1
IPC2_protein 4.304
IPC_protein 3.859
Toseland 3.795
ProMoST 3.999
Dawson 3.795
Bjellqvist 3.986
Wikipedia 3.694
Rodwell 3.745
Grimsley 3.732
Solomon 3.719
Lehninger 3.668
Nozaki 3.986
DTASelect 3.872
Thurlkill 3.846
EMBOSS 3.706
Sillero 3.973
Patrickios 0.006
IPC_peptide 3.732
IPC2_peptide 3.948
IPC2.peptide.svr19 3.898
Protein with the highest isoelectric point:
>tr|B2X7E0|B2X7E0_9RHAB GDP polyribonucleotidyltransferase OS=Wongabel hapavirus OX=1972626 GN=L PE=4 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.23 NLSSSVGDD10 pKa = 2.97 GHH12 pKa = 6.99 IWLKK16 pKa = 10.49 IHH18 pKa = 6.81 FLTMGFSINFDD29 pKa = 4.02 PIINKK34 pKa = 8.68 FRR36 pKa = 11.84 EE37 pKa = 3.97 FQTNINHH44 pKa = 7.14 NINEE48 pKa = 4.16 QLDD51 pKa = 3.97 KK52 pKa = 11.68 LKK54 pKa = 10.36 MVWINLGSHH63 pKa = 5.48 IKK65 pKa = 9.46 YY66 pKa = 9.07 WFIIIISILTILFILFLLIKK86 pKa = 8.77 ITKK89 pKa = 10.36 LILNCKK95 pKa = 10.01 KK96 pKa = 10.34 IFSCCCNVCCKK107 pKa = 10.12 KK108 pKa = 10.49 RR109 pKa = 11.84 PKK111 pKa = 10.36 VDD113 pKa = 2.75 IRR115 pKa = 11.84 SKK117 pKa = 10.64 EE118 pKa = 3.96 KK119 pKa = 10.92 VKK121 pKa = 10.79 VFSILPP127 pKa = 3.73
Molecular weight: 14.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.934
IPC2_protein 8.946
IPC_protein 8.829
Toseland 10.014
ProMoST 9.472
Dawson 10.101
Bjellqvist 9.736
Wikipedia 10.16
Rodwell 10.95
Grimsley 10.101
Solomon 10.131
Lehninger 10.131
Nozaki 10.101
DTASelect 9.663
Thurlkill 9.999
EMBOSS 10.379
Sillero 10.058
Patrickios 10.716
IPC_peptide 10.145
IPC2_peptide 8.448
IPC2.peptide.svr19 7.854
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
4313
49
2118
431.3
49.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.014 ± 0.244
1.832 ± 0.187
6.214 ± 0.518
5.959 ± 0.396
5.147 ± 0.322
5.449 ± 0.386
2.574 ± 0.251
8.579 ± 0.679
7.257 ± 0.33
9.97 ± 0.653
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.342 ± 0.325
5.912 ± 0.309
4.336 ± 0.272
3.107 ± 0.311
4.22 ± 0.222
7.976 ± 0.39
5.054 ± 0.586
4.846 ± 0.412
2.203 ± 0.148
4.011 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here