Cherry necrotic rusty mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Robigovirus

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9IH80|Q9IH80_9VIRU Helicase OS=Cherry necrotic rusty mottle virus OX=129143 GN=orf1 PE=3 SV=1
MM1 pKa = 6.52EE2 pKa = 5.73TIYY5 pKa = 11.19SRR7 pKa = 11.84LLNANFSRR15 pKa = 11.84TSFPISFPVVVHH27 pKa = 6.18GVPGCGKK34 pKa = 8.52STFIKK39 pKa = 10.7SLLDD43 pKa = 3.53CEE45 pKa = 4.52EE46 pKa = 5.17FSAQSYY52 pKa = 9.58GVVRR56 pKa = 11.84PTNLAGRR63 pKa = 11.84GVEE66 pKa = 4.54KK67 pKa = 10.45ALQPLQPGFNVLDD80 pKa = 4.62EE81 pKa = 4.4YY82 pKa = 11.6LSGPSYY88 pKa = 11.04EE89 pKa = 5.09GFDD92 pKa = 4.41LLLSDD97 pKa = 5.16PYY99 pKa = 11.53QNFRR103 pKa = 11.84KK104 pKa = 9.6PLTAHH109 pKa = 7.21FINSNTYY116 pKa = 8.28RR117 pKa = 11.84FGVSVCQFLNKK128 pKa = 10.25LGFEE132 pKa = 4.17INSCKK137 pKa = 10.68EE138 pKa = 3.43EE139 pKa = 3.99DD140 pKa = 3.62TEE142 pKa = 5.7LIFGKK147 pKa = 10.51VFGGEE152 pKa = 3.45IRR154 pKa = 11.84GEE156 pKa = 4.12IICFEE161 pKa = 4.38AEE163 pKa = 3.79VEE165 pKa = 4.29EE166 pKa = 4.38ILRR169 pKa = 11.84KK170 pKa = 9.86HH171 pKa = 5.36SAKK174 pKa = 9.95FHH176 pKa = 6.33HH177 pKa = 7.01PCNLRR182 pKa = 11.84GAEE185 pKa = 3.97FDD187 pKa = 3.3HH188 pKa = 6.11VTFITAHH195 pKa = 6.27QDD197 pKa = 3.0LQEE200 pKa = 4.22IVGPDD205 pKa = 3.72LYY207 pKa = 11.58VSLTRR212 pKa = 11.84ATKK215 pKa = 10.46SLTILSPP222 pKa = 3.64

Molecular weight: 24.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9IH75|Q9IH75_9VIRU Capsid protein OS=Cherry necrotic rusty mottle virus OX=129143 GN=orf5 PE=4 SV=1
MM1 pKa = 7.11MMEE4 pKa = 5.16PLNLMLQTTRR14 pKa = 11.84SRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LGQPLHH26 pKa = 6.02FPGMKK31 pKa = 9.51RR32 pKa = 11.84VLQEE36 pKa = 3.49RR37 pKa = 11.84AILKK41 pKa = 10.55FSDD44 pKa = 3.61QEE46 pKa = 4.2EE47 pKa = 4.94GEE49 pKa = 4.23LTSIQRR55 pKa = 11.84IPPQVLAEE63 pKa = 4.17SLSATFRR70 pKa = 11.84KK71 pKa = 8.61RR72 pKa = 11.84TLQLSTLHH80 pKa = 7.0LMTQLKK86 pKa = 9.1QLQPIGLSTSKK97 pKa = 10.6CLSLKK102 pKa = 10.47RR103 pKa = 11.84SIAFSMLSGTVTTTAQVTKK122 pKa = 9.83QNSWAEE128 pKa = 4.04PNVMSNLRR136 pKa = 11.84NWQALLGATALYY148 pKa = 10.32VAFVLNTRR156 pKa = 11.84QQ157 pKa = 3.07

Molecular weight: 17.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2985

67

2038

426.4

48.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.097 ± 0.48

2.01 ± 0.379

4.958 ± 1.013

6.365 ± 1.061

6.365 ± 0.816

5.595 ± 0.533

2.278 ± 0.429

5.863 ± 0.557

6.901 ± 1.544

10.888 ± 2.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.977 ± 0.449

4.757 ± 0.147

3.92 ± 0.793

3.149 ± 0.65

5.226 ± 0.638

9.313 ± 0.513

4.824 ± 1.06

5.796 ± 0.582

0.737 ± 0.235

2.982 ± 0.416
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski