Streptococcus phage P9902
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A286QS37|A0A286QS37_9CAUD Baseplate component OS=Streptococcus phage P9902 OX=1971448 GN=P9902_17 PE=4 SV=1
MM1 pKa = 7.3 SVSKK5 pKa = 9.08 EE6 pKa = 4.26 TIMQTLNLDD15 pKa = 3.8 EE16 pKa = 4.97 TDD18 pKa = 3.78 DD19 pKa = 3.8 TALIPAYY26 pKa = 9.65 IEE28 pKa = 3.9 SARR31 pKa = 11.84 QYY33 pKa = 10.3 VVNSVGDD40 pKa = 3.54 DD41 pKa = 3.37 PKK43 pKa = 10.95 FYY45 pKa = 11.07 NLDD48 pKa = 3.41 SVRR51 pKa = 11.84 ALFDD55 pKa = 3.34 TAVIALTSSYY65 pKa = 8.66 FTYY68 pKa = 10.07 RR69 pKa = 11.84 VALTDD74 pKa = 3.37 TATYY78 pKa = 9.41 PVNLTLNSIIGQLRR92 pKa = 11.84 GLYY95 pKa = 8.66 ATYY98 pKa = 10.56 SEE100 pKa = 4.46 EE101 pKa = 5.06 RR102 pKa = 11.84 GDD104 pKa = 3.53
Molecular weight: 11.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.863
IPC2_protein 4.151
IPC_protein 4.062
Toseland 3.859
ProMoST 4.139
Dawson 4.062
Bjellqvist 4.279
Wikipedia 3.999
Rodwell 3.897
Grimsley 3.77
Solomon 4.05
Lehninger 3.999
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.923
EMBOSS 4.012
Sillero 4.19
Patrickios 1.964
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.1
Protein with the highest isoelectric point:
>tr|A0A286QSR9|A0A286QSR9_9CAUD Uncharacterized protein OS=Streptococcus phage P9902 OX=1971448 GN=P9902_49 PE=4 SV=1
MM1 pKa = 8.11 DD2 pKa = 3.89 IKK4 pKa = 10.58 EE5 pKa = 4.03 IKK7 pKa = 10.23 GYY9 pKa = 10.07 EE10 pKa = 4.26 GLYY13 pKa = 9.36 EE14 pKa = 3.94 AHH16 pKa = 7.19 SDD18 pKa = 3.6 GTIWSCKK25 pKa = 9.91 NKK27 pKa = 8.02 TTYY30 pKa = 11.01 SFVRR34 pKa = 11.84 GKK36 pKa = 8.8 TIKK39 pKa = 10.38 RR40 pKa = 11.84 VWEE43 pKa = 3.68 QRR45 pKa = 11.84 EE46 pKa = 4.11 IKK48 pKa = 9.71 PQIARR53 pKa = 11.84 RR54 pKa = 11.84 QRR56 pKa = 11.84 SDD58 pKa = 3.8 HH59 pKa = 5.45 YY60 pKa = 11.24 DD61 pKa = 3.15 KK62 pKa = 11.02 RR63 pKa = 11.84 VKK65 pKa = 9.68 LWKK68 pKa = 8.83 NKK70 pKa = 9.59 KK71 pKa = 7.36 MTTHH75 pKa = 6.29 LVSRR79 pKa = 11.84 LIAQTFIPNPEE90 pKa = 3.65 NKK92 pKa = 10.32 GYY94 pKa = 11.08 VNHH97 pKa = 7.25 KK98 pKa = 10.04 NGNPLDD104 pKa = 3.9 NSVEE108 pKa = 3.87 NLEE111 pKa = 4.1 WVTRR115 pKa = 11.84 SEE117 pKa = 4.15 NMRR120 pKa = 11.84 HH121 pKa = 5.33 AFKK124 pKa = 11.0 NGLLQTSKK132 pKa = 11.06 KK133 pKa = 7.78 VTLVSKK139 pKa = 11.12 ADD141 pKa = 3.5 GAKK144 pKa = 10.11 VSFYY148 pKa = 10.43 SLRR151 pKa = 11.84 AASEE155 pKa = 3.92 FLGKK159 pKa = 10.32 NKK161 pKa = 10.31 GYY163 pKa = 10.24 LSNIIKK169 pKa = 10.41 SGRR172 pKa = 11.84 TLGNYY177 pKa = 8.72 EE178 pKa = 3.99 IVVGEE183 pKa = 4.0 II184 pKa = 3.37
Molecular weight: 21.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.086
IPC2_protein 9.37
IPC_protein 9.311
Toseland 10.175
ProMoST 9.75
Dawson 10.321
Bjellqvist 9.94
Wikipedia 10.452
Rodwell 10.921
Grimsley 10.379
Solomon 10.335
Lehninger 10.321
Nozaki 10.145
DTASelect 9.94
Thurlkill 10.189
EMBOSS 10.555
Sillero 10.233
Patrickios 10.599
IPC_peptide 10.35
IPC2_peptide 8.404
IPC2.peptide.svr19 8.416
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
50
0
50
11198
33
1656
224.0
25.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.483 ± 0.408
0.563 ± 0.122
6.314 ± 0.234
7.001 ± 0.537
4.349 ± 0.192
6.439 ± 0.458
1.438 ± 0.118
6.457 ± 0.198
8.627 ± 0.524
8.091 ± 0.371
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.483 ± 0.141
6.626 ± 0.307
2.911 ± 0.17
4.385 ± 0.239
4.063 ± 0.23
6.233 ± 0.276
6.001 ± 0.244
5.983 ± 0.287
1.348 ± 0.145
4.206 ± 0.322
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here