Torque teno mini virus 11
Average proteome isoelectric point is 7.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8DMQ9|A8DMQ9_9VIRU Capsid protein OS=Torque teno mini virus 11 OX=2065037 PE=3 SV=1
MM1 pKa = 7.47 SYY3 pKa = 10.56 KK4 pKa = 10.09 PPRR7 pKa = 11.84 YY8 pKa = 9.37 GPQGLEE14 pKa = 3.76 LQFLNSIFTNHH25 pKa = 7.22 DD26 pKa = 3.97 LFCGCDD32 pKa = 3.34 DD33 pKa = 3.9 TLKK36 pKa = 10.77 HH37 pKa = 6.21 ILSIFNKK44 pKa = 8.89 QNKK47 pKa = 9.46 ANLSDD52 pKa = 3.61 TKK54 pKa = 10.56 IQEE57 pKa = 4.31 IKK59 pKa = 10.69 CLLTGEE65 pKa = 4.35 TTEE68 pKa = 4.66 TGDD71 pKa = 4.62 KK72 pKa = 10.92 GDD74 pKa = 3.72 GAAGVLDD81 pKa = 5.08 AIEE84 pKa = 5.79 DD85 pKa = 3.84 GDD87 pKa = 4.45 LDD89 pKa = 4.6 ALFEE93 pKa = 5.04 LSDD96 pKa = 6.07 DD97 pKa = 3.61 MDD99 pKa = 4.01 EE100 pKa = 4.68 PNGG103 pKa = 3.73
Molecular weight: 11.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.228
IPC2_protein 4.101
IPC_protein 4.062
Toseland 3.846
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.986
Rodwell 3.884
Grimsley 3.757
Solomon 4.037
Lehninger 3.999
Nozaki 4.164
DTASelect 4.406
Thurlkill 3.897
EMBOSS 3.999
Sillero 4.177
Patrickios 3.567
IPC_peptide 4.037
IPC2_peptide 4.151
IPC2.peptide.svr19 4.101
Protein with the highest isoelectric point:
>tr|A8DMR0|A8DMR0_9VIRU ORF3 OS=Torque teno mini virus 11 OX=2065037 PE=4 SV=1
MM1 pKa = 7.52 IPVHH5 pKa = 5.74 SQNGPQPVTSNKK17 pKa = 7.76 QFRR20 pKa = 11.84 SRR22 pKa = 11.84 IQNTTQATTSTLGTLEE38 pKa = 4.05 GTFLQTQLSKK48 pKa = 11.03 DD49 pKa = 3.39 LKK51 pKa = 9.18 KK52 pKa = 9.31 TKK54 pKa = 10.67 KK55 pKa = 10.29 LMRR58 pKa = 11.84 LFQLKK63 pKa = 9.14 QAQKK67 pKa = 8.6 AAKK70 pKa = 8.87 RR71 pKa = 11.84 RR72 pKa = 11.84 SPSPPNTPRR81 pKa = 11.84 KK82 pKa = 9.36 LRR84 pKa = 11.84 RR85 pKa = 11.84 SIDD88 pKa = 3.31 TGKK91 pKa = 10.6 KK92 pKa = 9.66 EE93 pKa = 4.45 RR94 pKa = 11.84 DD95 pKa = 3.29 TAAQAPQTPTKK106 pKa = 10.41 PPATTAAAHH115 pKa = 6.09 QIPKK119 pKa = 8.45 ITIIHH124 pKa = 6.25 KK125 pKa = 9.3 PVKK128 pKa = 10.01 INLFNDD134 pKa = 3.78 PVPVKK139 pKa = 10.57 NRR141 pKa = 11.84 PFKK144 pKa = 8.89 PWEE147 pKa = 3.82 WEE149 pKa = 3.85 DD150 pKa = 3.54 EE151 pKa = 4.15 KK152 pKa = 11.47 FIASWLKK159 pKa = 10.03 RR160 pKa = 11.84 PQRR163 pKa = 11.84 HH164 pKa = 4.94 YY165 pKa = 10.71 FYY167 pKa = 10.87 DD168 pKa = 3.71 KK169 pKa = 8.61 PTYY172 pKa = 9.64 PIFPITPLANFDD184 pKa = 4.92 LNFKK188 pKa = 9.75 EE189 pKa = 4.32
Molecular weight: 21.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.207
IPC2_protein 9.765
IPC_protein 9.97
Toseland 10.906
ProMoST 10.394
Dawson 10.965
Bjellqvist 10.555
Wikipedia 11.082
Rodwell 11.506
Grimsley 10.979
Solomon 11.023
Lehninger 11.008
Nozaki 10.862
DTASelect 10.555
Thurlkill 10.877
EMBOSS 11.272
Sillero 10.877
Patrickios 11.228
IPC_peptide 11.038
IPC2_peptide 8.946
IPC2.peptide.svr19 8.759
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
954
103
662
318.0
36.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.403 ± 0.948
1.363 ± 0.536
5.451 ± 1.783
3.774 ± 0.752
4.298 ± 0.431
4.927 ± 1.351
1.782 ± 0.141
5.556 ± 0.136
9.434 ± 0.885
7.442 ± 1.304
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.468 ± 0.162
6.184 ± 0.556
7.128 ± 1.605
4.927 ± 0.862
5.975 ± 1.272
7.652 ± 1.458
8.071 ± 0.874
2.935 ± 0.555
2.621 ± 0.878
4.612 ± 1.478
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here