Gordonia phage NatB6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345L515|A0A345L515_9CAUD Uncharacterized protein OS=Gordonia phage NatB6 OX=2250322 GN=87 PE=4 SV=1
MM1 pKa = 6.62THH3 pKa = 6.46SPAASDD9 pKa = 3.98VIATRR14 pKa = 11.84TLDD17 pKa = 3.7PVDD20 pKa = 4.51GFTLTAFEE28 pKa = 4.91VPDD31 pKa = 4.04TDD33 pKa = 3.76STPIGQSLDD42 pKa = 3.24EE43 pKa = 4.79GGCYY47 pKa = 8.92TADD50 pKa = 5.31DD51 pKa = 3.72IEE53 pKa = 4.04AWRR56 pKa = 11.84RR57 pKa = 11.84DD58 pKa = 2.76EE59 pKa = 3.88WQYY62 pKa = 11.21VGVVVVASRR71 pKa = 11.84AGVDD75 pKa = 3.79LGSASIWGMDD85 pKa = 3.27TGDD88 pKa = 3.48YY89 pKa = 10.09WDD91 pKa = 5.58AITRR95 pKa = 11.84ADD97 pKa = 3.04GRR99 pKa = 11.84TPYY102 pKa = 10.7GVLDD106 pKa = 3.78NVPNSVTTGHH116 pKa = 6.78PQPDD120 pKa = 3.54GTIDD124 pKa = 3.34YY125 pKa = 7.52VTRR128 pKa = 11.84DD129 pKa = 4.14DD130 pKa = 3.93FAHH133 pKa = 7.2GYY135 pKa = 9.33GADD138 pKa = 4.51LISEE142 pKa = 4.69ALTEE146 pKa = 4.09ARR148 pKa = 11.84ATVAALTSPDD158 pKa = 3.52VVIPVCDD165 pKa = 3.49EE166 pKa = 3.96CGEE169 pKa = 4.22RR170 pKa = 11.84ADD172 pKa = 4.52GVSDD176 pKa = 3.42AHH178 pKa = 6.9GVACSLFAGNVAGYY192 pKa = 8.15TDD194 pKa = 4.79EE195 pKa = 5.01VPAPTFYY202 pKa = 10.52PGAGGWHH209 pKa = 5.59TEE211 pKa = 3.81QRR213 pKa = 11.84GTCTYY218 pKa = 10.45HH219 pKa = 5.82VATIAGGHH227 pKa = 5.79RR228 pKa = 11.84AAYY231 pKa = 7.83VTLNDD236 pKa = 3.43ANEE239 pKa = 4.27YY240 pKa = 10.09VAGLYY245 pKa = 7.74DD246 pKa = 3.76TTDD249 pKa = 3.39TPHH252 pKa = 7.12VIGTFPTLFDD262 pKa = 3.78GKK264 pKa = 10.04RR265 pKa = 11.84AADD268 pKa = 3.53AARR271 pKa = 11.84EE272 pKa = 4.02RR273 pKa = 11.84GSVKK277 pKa = 10.58

Molecular weight: 29.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345L4V1|A0A345L4V1_9CAUD HNH endonuclease OS=Gordonia phage NatB6 OX=2250322 GN=23 PE=4 SV=1
MM1 pKa = 6.82ATRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84GGSLNTRR14 pKa = 11.84KK15 pKa = 9.98KK16 pKa = 10.82GKK18 pKa = 10.05AKK20 pKa = 10.66NGAKK24 pKa = 9.07WGHH27 pKa = 5.7GFVPKK32 pKa = 10.29NAAARR37 pKa = 11.84KK38 pKa = 9.03LKK40 pKa = 10.66KK41 pKa = 10.41KK42 pKa = 10.3LDD44 pKa = 3.9RR45 pKa = 11.84NGKK48 pKa = 7.93RR49 pKa = 11.84RR50 pKa = 11.84KK51 pKa = 8.79RR52 pKa = 11.84TSQGYY57 pKa = 7.24PKK59 pKa = 10.37KK60 pKa = 10.56RR61 pKa = 3.29

Molecular weight: 6.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

21352

41

1797

229.6

25.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.126 ± 0.427

0.852 ± 0.131

7.255 ± 0.314

6.487 ± 0.277

3.082 ± 0.142

8.585 ± 0.372

1.911 ± 0.133

4.927 ± 0.22

4.093 ± 0.286

7.395 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.262 ± 0.105

3.11 ± 0.167

5.798 ± 0.201

3.166 ± 0.148

7.634 ± 0.329

5.255 ± 0.189

6.449 ± 0.225

7.105 ± 0.199

1.939 ± 0.116

2.571 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski