Capybara microvirus Cap3_SP_391
Average proteome isoelectric point is 5.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8H6|A0A4P8W8H6_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_391 OX=2585442 PE=4 SV=1
MM1 pKa = 7.98 DD2 pKa = 4.37 SEE4 pKa = 4.04 EE5 pKa = 5.07 DD6 pKa = 3.26 IYY8 pKa = 11.61 EE9 pKa = 4.46 KK10 pKa = 10.55 IQADD14 pKa = 3.95 LEE16 pKa = 4.27 DD17 pKa = 4.2 SKK19 pKa = 11.31 IEE21 pKa = 4.11 NIIQRR26 pKa = 11.84 VEE28 pKa = 4.04 YY29 pKa = 10.28 GDD31 pKa = 3.83 VGALSARR38 pKa = 11.84 QGIYY42 pKa = 10.69 ADD44 pKa = 3.5 IADD47 pKa = 5.0 MPTSWAEE54 pKa = 3.89 AQTKK58 pKa = 7.39 ITAMEE63 pKa = 4.1 QIFNKK68 pKa = 10.37 LPLEE72 pKa = 3.99 VRR74 pKa = 11.84 KK75 pKa = 10.06 AYY77 pKa = 10.45 DD78 pKa = 3.26 FNFRR82 pKa = 11.84 AFIADD87 pKa = 3.62 YY88 pKa = 11.05 GSDD91 pKa = 3.19 QFMEE95 pKa = 4.17 ALGVHH100 pKa = 6.43 RR101 pKa = 11.84 EE102 pKa = 4.18 GKK104 pKa = 9.54 PVEE107 pKa = 4.1 QPAAEE112 pKa = 4.12 KK113 pKa = 10.74 PEE115 pKa = 4.35 GGEE118 pKa = 4.11 AEE120 pKa = 4.15
Molecular weight: 13.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.42
IPC2_protein 4.291
IPC_protein 4.215
Toseland 4.037
ProMoST 4.279
Dawson 4.151
Bjellqvist 4.342
Wikipedia 4.024
Rodwell 4.037
Grimsley 3.948
Solomon 4.151
Lehninger 4.101
Nozaki 4.266
DTASelect 4.406
Thurlkill 4.05
EMBOSS 4.037
Sillero 4.317
Patrickios 3.821
IPC_peptide 4.151
IPC2_peptide 4.304
IPC2.peptide.svr19 4.25
Protein with the highest isoelectric point:
>tr|A0A4P8W4W5|A0A4P8W4W5_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_391 OX=2585442 PE=3 SV=1
MM1 pKa = 7.71 AWRR4 pKa = 11.84 AYY6 pKa = 9.63 CIAWQPITGCSRR18 pKa = 11.84 NCSKK22 pKa = 10.52 IAAGTRR28 pKa = 11.84 RR29 pKa = 11.84 AYY31 pKa = 10.28 RR32 pKa = 11.84 KK33 pKa = 9.3 QRR35 pKa = 11.84 WGLMRR40 pKa = 11.84 CSHH43 pKa = 7.28 PLMAFYY49 pKa = 10.09 TIDD52 pKa = 3.54 PVTGRR57 pKa = 11.84 KK58 pKa = 8.35 NLRR61 pKa = 11.84 HH62 pKa = 6.03 FKK64 pKa = 10.84 SIDD67 pKa = 3.42 TADD70 pKa = 3.14 GKK72 pKa = 10.76 AIYY75 pKa = 9.01 EE76 pKa = 4.05 LHH78 pKa = 6.03 DD79 pKa = 3.49 QEE81 pKa = 4.34 RR82 pKa = 11.84 TIMIPCGQCLACRR95 pKa = 11.84 MRR97 pKa = 11.84 YY98 pKa = 9.78 SNDD101 pKa = 2.75 WANRR105 pKa = 11.84 IYY107 pKa = 10.98 LEE109 pKa = 4.22 ACTYY113 pKa = 10.31 PRR115 pKa = 11.84 DD116 pKa = 4.08 RR117 pKa = 11.84 NWFLTLTYY125 pKa = 10.84 DD126 pKa = 4.76 EE127 pKa = 5.18 DD128 pKa = 3.85 HH129 pKa = 6.82 LPYY132 pKa = 10.78 GSGEE136 pKa = 4.16 RR137 pKa = 11.84 PTLIKK142 pKa = 10.93 DD143 pKa = 3.85 EE144 pKa = 4.2 ISKK147 pKa = 10.17 FMKK150 pKa = 10.38 ALRR153 pKa = 11.84 QRR155 pKa = 11.84 WAEE158 pKa = 4.4 DD159 pKa = 3.2 EE160 pKa = 4.36 TQTEE164 pKa = 5.01 GIRR167 pKa = 11.84 FFGCGEE173 pKa = 4.15 YY174 pKa = 10.58 GGKK177 pKa = 6.81 TQRR180 pKa = 11.84 PHH182 pKa = 4.92 YY183 pKa = 10.26 HH184 pKa = 7.05 IILLNCLIKK193 pKa = 10.74 KK194 pKa = 10.03 EE195 pKa = 3.95 DD196 pKa = 3.48 LKK198 pKa = 11.34 VYY200 pKa = 8.84 KK201 pKa = 10.28 TEE203 pKa = 4.44 AGHH206 pKa = 6.4 VLWNVPKK213 pKa = 9.46 ITHH216 pKa = 5.64 IWGKK220 pKa = 8.07 GHH222 pKa = 6.5 VVVAEE227 pKa = 4.65 CNWEE231 pKa = 4.03 TCAYY235 pKa = 7.74 TSRR238 pKa = 11.84 YY239 pKa = 7.47 MLKK242 pKa = 9.72 KK243 pKa = 10.14 HH244 pKa = 6.26 KK245 pKa = 10.68 GQDD248 pKa = 2.65 ADD250 pKa = 3.78 YY251 pKa = 9.76 YY252 pKa = 9.18 EE253 pKa = 4.54 KK254 pKa = 11.04 LGIYY258 pKa = 9.31 PEE260 pKa = 4.07 HH261 pKa = 6.25 TRR263 pKa = 11.84 MSRR266 pKa = 11.84 RR267 pKa = 11.84 PGIGADD273 pKa = 3.51 YY274 pKa = 11.14 LDD276 pKa = 5.61 DD277 pKa = 3.45 NWEE280 pKa = 4.33 QIWSRR285 pKa = 11.84 DD286 pKa = 3.32 HH287 pKa = 7.87 ILIKK291 pKa = 10.29 GHH293 pKa = 5.14 QAAVPAAYY301 pKa = 8.42 TRR303 pKa = 11.84 KK304 pKa = 10.08 LGYY307 pKa = 9.99 SDD309 pKa = 5.1 LDD311 pKa = 3.48 RR312 pKa = 11.84 EE313 pKa = 4.56 AEE315 pKa = 4.0 IKK317 pKa = 9.96 AKK319 pKa = 10.24 RR320 pKa = 11.84 MHH322 pKa = 6.83 RR323 pKa = 11.84 AQLTAEE329 pKa = 4.03 NEE331 pKa = 4.36 ANLGAYY337 pKa = 7.9 DD338 pKa = 3.81 RR339 pKa = 11.84 PVWEE343 pKa = 3.81 QLQIEE348 pKa = 4.57 EE349 pKa = 3.93 EE350 pKa = 4.16 AMRR353 pKa = 11.84 QRR355 pKa = 11.84 TALLKK360 pKa = 10.43 RR361 pKa = 11.84 RR362 pKa = 11.84 LEE364 pKa = 3.98 KK365 pKa = 10.96 GILKK369 pKa = 10.11 KK370 pKa = 10.42 KK371 pKa = 7.55 YY372 pKa = 9.26 HH373 pKa = 6.49 LYY375 pKa = 10.36 EE376 pKa = 4.69 GSLII380 pKa = 3.97
Molecular weight: 44.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.544
IPC2_protein 8.463
IPC_protein 8.331
Toseland 8.668
ProMoST 8.829
Dawson 9.151
Bjellqvist 9.18
Wikipedia 9.282
Rodwell 9.253
Grimsley 9.033
Solomon 9.209
Lehninger 9.18
Nozaki 9.282
DTASelect 9.019
Thurlkill 9.092
EMBOSS 9.282
Sillero 9.311
Patrickios 4.342
IPC_peptide 9.194
IPC2_peptide 8.068
IPC2.peptide.svr19 7.805
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1413
82
579
282.6
31.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.13 ± 1.16
1.132 ± 0.658
5.591 ± 0.313
6.369 ± 1.064
3.043 ± 0.542
7.573 ± 1.065
1.84 ± 0.762
5.662 ± 0.711
5.308 ± 0.739
6.369 ± 0.662
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.609 ± 0.548
4.105 ± 0.588
4.388 ± 0.8
5.096 ± 0.455
5.449 ± 1.206
6.794 ± 1.762
6.369 ± 1.074
4.388 ± 0.779
2.265 ± 0.514
5.52 ± 0.686
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here