Vibrio virus fs1
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|O56849|O56849_9VIRU Uncharacterized protein OS=Vibrio virus fs1 OX=70203 PE=4 SV=1
MM1 pKa = 7.51 PVCALPNSQGFLAVTDD17 pKa = 4.27 KK18 pKa = 10.95 PLNEE22 pKa = 4.31 CDD24 pKa = 3.24 GGYY27 pKa = 10.23 VAVTIQDD34 pKa = 3.3 YY35 pKa = 10.53 DD36 pKa = 3.69 YY37 pKa = 11.27 LMSYY41 pKa = 8.66 TRR43 pKa = 11.84 ITPTDD48 pKa = 3.19 AGTAFSFGFMAVFALGYY65 pKa = 9.32 LYY67 pKa = 10.33 TYY69 pKa = 10.88 AVYY72 pKa = 10.17 IGKK75 pKa = 9.83 KK76 pKa = 10.08 LINLLL81 pKa = 3.88
Molecular weight: 8.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.441
IPC2_protein 4.596
IPC_protein 4.291
Toseland 4.075
ProMoST 4.482
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.291
Rodwell 4.113
Grimsley 3.986
Solomon 4.279
Lehninger 4.24
Nozaki 4.444
DTASelect 4.711
Thurlkill 4.164
EMBOSS 4.291
Sillero 4.418
Patrickios 0.006
IPC_peptide 4.266
IPC2_peptide 4.38
IPC2.peptide.svr19 4.352
Protein with the highest isoelectric point:
>tr|O56842|O56842_9VIRU Uncharacterized protein OS=Vibrio virus fs1 OX=70203 PE=4 SV=1
MM1 pKa = 7.85 IINTLAPEE9 pKa = 5.2 KK10 pKa = 10.6 IPFALPSHH18 pKa = 6.26 KK19 pKa = 10.25 RR20 pKa = 11.84 CFSKK24 pKa = 10.68 RR25 pKa = 11.84 SSS27 pKa = 3.13
Molecular weight: 3.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.201
IPC2_protein 9.399
IPC_protein 9.545
Toseland 10.701
ProMoST 10.101
Dawson 10.745
Bjellqvist 10.306
Wikipedia 10.818
Rodwell 11.33
Grimsley 10.745
Solomon 10.818
Lehninger 10.818
Nozaki 10.687
DTASelect 10.292
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.657
Patrickios 11.199
IPC_peptide 10.833
IPC2_peptide 9.151
IPC2.peptide.svr19 8.405
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
1804
20
384
120.3
13.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.929 ± 0.78
1.829 ± 0.394
5.71 ± 0.56
4.268 ± 0.607
4.989 ± 0.525
5.82 ± 0.51
1.996 ± 0.642
7.761 ± 0.797
6.375 ± 0.514
9.035 ± 1.157
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.882 ± 0.482
4.435 ± 0.633
5.765 ± 0.665
3.991 ± 0.481
4.157 ± 0.642
7.151 ± 0.831
6.098 ± 0.671
5.931 ± 0.377
1.109 ± 0.271
3.769 ± 0.796
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here