Oenococcus phage phi9805
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V9QKK2|V9QKK2_9CAUD Hol44 OS=Oenococcus phage phi9805 OX=1435411 PE=4 SV=1
MM1 pKa = 7.89 IIQTTAATAAWKK13 pKa = 10.29 ASQRR17 pKa = 11.84 TLDD20 pKa = 3.6 TVVTIDD26 pKa = 3.16 GTDD29 pKa = 3.33 YY30 pKa = 9.06 HH31 pKa = 6.65 TSDD34 pKa = 3.74 ITSIAYY40 pKa = 9.97 DD41 pKa = 3.62 GGAFTGDD48 pKa = 3.31 TFSIGSTYY56 pKa = 10.31 EE57 pKa = 3.64 NSVTITFSHH66 pKa = 6.17 LVEE69 pKa = 5.0 GFVQGQLVAPKK80 pKa = 10.49 VGVKK84 pKa = 10.36 LVDD87 pKa = 3.42 GTFEE91 pKa = 4.01 YY92 pKa = 10.93 SPLGIFVISDD102 pKa = 4.21 DD103 pKa = 4.9 IEE105 pKa = 3.89 MDD107 pKa = 3.46 RR108 pKa = 11.84 NNDD111 pKa = 2.81 VTTIKK116 pKa = 10.54 AYY118 pKa = 10.66 DD119 pKa = 4.11 LMCMLEE125 pKa = 4.01 GTYY128 pKa = 9.19 TSKK131 pKa = 9.9 LTYY134 pKa = 7.87 PAKK137 pKa = 8.12 MTDD140 pKa = 3.85 VIAEE144 pKa = 4.08 IASLSGVPLNSDD156 pKa = 4.19 DD157 pKa = 4.11 IARR160 pKa = 11.84 LPLMNNLAKK169 pKa = 10.65 AITGQTYY176 pKa = 10.24 RR177 pKa = 11.84 NAIGWIAQFYY187 pKa = 10.73 SGFALFDD194 pKa = 3.89 RR195 pKa = 11.84 DD196 pKa = 3.32 GKK198 pKa = 9.56 LTIRR202 pKa = 11.84 TINDD206 pKa = 2.95 TDD208 pKa = 3.81 YY209 pKa = 11.61 VIDD212 pKa = 4.34 AGQYY216 pKa = 9.21 LQGGLTKK223 pKa = 10.76 NEE225 pKa = 3.54 AAYY228 pKa = 10.7 VIGGIQCQVTTTTTDD243 pKa = 2.75 SDD245 pKa = 4.18 GNSTDD250 pKa = 3.71 DD251 pKa = 3.7 TVTLQSGSRR260 pKa = 11.84 AGSQVQLTNNVMTQEE275 pKa = 4.1 RR276 pKa = 11.84 LDD278 pKa = 4.65 AIWTKK283 pKa = 10.53 LQNLAFYY290 pKa = 9.68 PFSLNWFGNPAIEE303 pKa = 4.63 AGDD306 pKa = 3.58 WFALQDD312 pKa = 3.65 TKK314 pKa = 11.55 GNRR317 pKa = 11.84 FNVPNCSYY325 pKa = 11.39 TMTFDD330 pKa = 3.83 GSFSSVSSAQQTSTSSDD347 pKa = 2.72 IYY349 pKa = 10.98 SYY351 pKa = 11.6 NGDD354 pKa = 3.47 LTSAINKK361 pKa = 8.72 LKK363 pKa = 10.85 SQTAGLNSYY372 pKa = 7.37 THH374 pKa = 6.71 IAYY377 pKa = 10.15 ADD379 pKa = 3.81 DD380 pKa = 3.67 ATGKK384 pKa = 10.55 GFSQDD389 pKa = 3.65 PTGKK393 pKa = 10.27 AYY395 pKa = 10.84 LGVYY399 pKa = 8.75 TDD401 pKa = 4.08 SNSVDD406 pKa = 3.28 STDD409 pKa = 3.42 PAKK412 pKa = 10.52 YY413 pKa = 10.26 VWMQTRR419 pKa = 11.84 GSDD422 pKa = 4.02 GEE424 pKa = 4.88 DD425 pKa = 3.38 GTSVEE430 pKa = 4.58 SSTVAYY436 pKa = 9.97 QAATSGTITPTGTWASDD453 pKa = 3.39 IPVVPAGQYY462 pKa = 10.47 LWTKK466 pKa = 8.69 TVFHH470 pKa = 6.63 YY471 pKa = 11.37 SNDD474 pKa = 3.87 STSTTYY480 pKa = 10.57 SVSYY484 pKa = 10.55 FGTNGSNGTAGIPGADD500 pKa = 2.94 GKK502 pKa = 9.09 TPYY505 pKa = 10.38 FHH507 pKa = 6.79 TAYY510 pKa = 9.34 STASDD515 pKa = 3.67 GSTGFSTTDD524 pKa = 3.44 PTNKK528 pKa = 9.51 TYY530 pKa = 11.01 LGTYY534 pKa = 10.4 SDD536 pKa = 4.18 FTQADD541 pKa = 3.53 STNAVSYY548 pKa = 10.8 DD549 pKa = 3.04 WVKK552 pKa = 10.71 IQGNTGATGATGATGKK568 pKa = 10.8 GITSTVITYY577 pKa = 8.28 QASTSGTVAPSGTWSSSIPSVSASQYY603 pKa = 8.6 LWTRR607 pKa = 11.84 TVMTFSDD614 pKa = 4.47 GASTTSYY621 pKa = 10.74 SVGMMGATGAKK632 pKa = 9.86 GDD634 pKa = 4.17 TGVQGIPGANGQSSYY649 pKa = 10.98 LHH651 pKa = 6.26 IAYY654 pKa = 10.0 ADD656 pKa = 3.71 SADD659 pKa = 3.19 GKK661 pKa = 8.61 TNFSITSAGLRR672 pKa = 11.84 QYY674 pKa = 10.8 IGTYY678 pKa = 9.38 TDD680 pKa = 3.83 FTSTSSSDD688 pKa = 3.07 PTKK691 pKa = 10.87 YY692 pKa = 10.19 VWQQTKK698 pKa = 10.71 GDD700 pKa = 3.73 TGAQGPAGTDD710 pKa = 2.85 ITAITYY716 pKa = 10.11 GSTAPSSPKK725 pKa = 10.68 EE726 pKa = 3.45 GDD728 pKa = 2.84 VWYY731 pKa = 10.07 KK732 pKa = 10.93 PNGNSIQIEE741 pKa = 4.48 IYY743 pKa = 10.84 HH744 pKa = 5.96 NGSWVLDD751 pKa = 3.44 IDD753 pKa = 4.68 DD754 pKa = 5.83 SIGQRR759 pKa = 11.84 ITDD762 pKa = 3.45 ATKK765 pKa = 10.86 DD766 pKa = 3.57 VLTSAKK772 pKa = 9.93 TYY774 pKa = 10.77 TDD776 pKa = 3.42 DD777 pKa = 3.35 TAKK780 pKa = 10.89 NVIAEE785 pKa = 4.43 LSSANKK791 pKa = 9.61 IVNSEE796 pKa = 3.98 FDD798 pKa = 3.02 TDD800 pKa = 3.7 TAQKK804 pKa = 10.75 NVVEE808 pKa = 4.66 SSTTVPTPNSKK819 pKa = 10.43 GYY821 pKa = 10.96 ADD823 pKa = 3.47 QTIVGRR829 pKa = 11.84 NLLRR833 pKa = 11.84 NTSAFANTDD842 pKa = 2.62 HH843 pKa = 7.15 WSNSGGGSSLAIVSRR858 pKa = 11.84 AFYY861 pKa = 10.85 QNGQGKK867 pKa = 8.7 LLKK870 pKa = 10.36 LSTSGTTEE878 pKa = 3.65 VFLMSEE884 pKa = 4.23 HH885 pKa = 6.63 FSVQAGEE892 pKa = 4.62 SYY894 pKa = 10.28 TFQIKK899 pKa = 10.37 AFNNSNVVSMDD910 pKa = 3.21 FFALGRR916 pKa = 11.84 QTGSTSDD923 pKa = 3.54 YY924 pKa = 9.1 TKK926 pKa = 10.94 VVASKK931 pKa = 11.2 LSMQPSISGIGTFSFSFTVPDD952 pKa = 5.21 GIGEE956 pKa = 4.27 LYY958 pKa = 10.27 IRR960 pKa = 11.84 IDD962 pKa = 3.61 NNGSKK967 pKa = 10.72 VSGSSADD974 pKa = 3.67 LYY976 pKa = 9.08 VAEE979 pKa = 4.97 MKK981 pKa = 10.99 LEE983 pKa = 4.23 VGSLTPYY990 pKa = 10.12 IKK992 pKa = 10.66 APEE995 pKa = 4.37 DD996 pKa = 3.11 LAIANWQDD1004 pKa = 3.53 TIVNDD1009 pKa = 4.39 PVDD1012 pKa = 3.43 GTYY1015 pKa = 10.57 VVSGTTSTKK1024 pKa = 10.76 ASTVQVKK1031 pKa = 10.17 NSAGTIVASQDD1042 pKa = 3.05 LSQSKK1047 pKa = 10.78 NSDD1050 pKa = 3.09 QTFTRR1055 pKa = 11.84 GSFSAVTHH1063 pKa = 6.97 DD1064 pKa = 4.18 NDD1066 pKa = 5.37 DD1067 pKa = 3.87 GTGTASIPTPTAGTSLIVVDD1087 pKa = 4.66 EE1088 pKa = 4.4 LNKK1091 pKa = 10.63 NIYY1094 pKa = 10.0 SNNNMSVPNLARR1106 pKa = 11.84 DD1107 pKa = 3.59 SEE1109 pKa = 4.41 MLLGLSGTDD1118 pKa = 3.25 SDD1120 pKa = 4.04 PHH1122 pKa = 7.44 FSYY1125 pKa = 11.35 NLADD1129 pKa = 3.97 FVSIVANGYY1138 pKa = 9.24 NGHH1141 pKa = 6.32 NVLDD1145 pKa = 4.2 IKK1147 pKa = 10.69 RR1148 pKa = 11.84 SSGSGTLVAITAYY1161 pKa = 10.13 QNSVPGDD1168 pKa = 3.25 AWSMGFYY1175 pKa = 10.61 YY1176 pKa = 9.98 RR1177 pKa = 11.84 VLTDD1181 pKa = 3.25 TTFSNDD1187 pKa = 2.71 SSCYY1191 pKa = 9.17 FDD1193 pKa = 4.43 PRR1195 pKa = 11.84 TSTGTAATVQGKK1207 pKa = 8.13 TILTAGTTWRR1217 pKa = 11.84 YY1218 pKa = 9.76 VKK1220 pKa = 10.88 VEE1222 pKa = 4.09 DD1223 pKa = 4.3 AVMPSTTAKK1232 pKa = 9.84 VRR1234 pKa = 11.84 FRR1236 pKa = 11.84 FDD1238 pKa = 3.08 MLGTGHH1244 pKa = 6.63 IQIALPMMVKK1254 pKa = 10.24 AVKK1257 pKa = 10.31 AVDD1260 pKa = 3.88 YY1261 pKa = 11.24 VSDD1264 pKa = 4.18 TIDD1267 pKa = 3.14 VSKK1270 pKa = 10.52 WVATLPADD1278 pKa = 3.81 GKK1280 pKa = 10.48 SYY1282 pKa = 10.89 KK1283 pKa = 9.75 ATIKK1287 pKa = 10.68 APKK1290 pKa = 8.78 DD1291 pKa = 3.29 TTATILNFNMPTAIDD1306 pKa = 3.83 RR1307 pKa = 11.84 YY1308 pKa = 9.06 NFSFSFPAVMGNYY1321 pKa = 8.56 TLALSEE1327 pKa = 4.08 ATYY1330 pKa = 11.19 SFTVQQNLFPVTSNGYY1346 pKa = 8.43 VVSKK1350 pKa = 9.72 TGVISGKK1357 pKa = 10.37 APNNTSVIYY1366 pKa = 10.43 LLSSSGGSYY1375 pKa = 8.93 STGVQSDD1382 pKa = 3.85 GSFAIAVNTDD1392 pKa = 2.45 GSGYY1396 pKa = 8.74 TIHH1399 pKa = 7.56 AEE1401 pKa = 4.08 YY1402 pKa = 9.2 QQSYY1406 pKa = 10.69 LGLTDD1411 pKa = 3.04 WHH1413 pKa = 7.04 DD1414 pKa = 4.13 FSPDD1418 pKa = 3.45 LPASSYY1424 pKa = 10.74 ISANEE1429 pKa = 3.8 LVSGSKK1435 pKa = 10.52 VIEE1438 pKa = 4.07 VTNNTLYY1445 pKa = 11.16 ADD1447 pKa = 5.87 DD1448 pKa = 4.71 IPSFANVAISVGAQFKK1464 pKa = 10.56 LISGTAKK1471 pKa = 10.38 LAVVFYY1477 pKa = 11.01 KK1478 pKa = 10.77 NDD1480 pKa = 3.44 GTNLGTQSVSIAGSDD1495 pKa = 3.1 WTSFSLINIVTPANVDD1511 pKa = 3.71 YY1512 pKa = 10.94 IRR1514 pKa = 11.84 VMVQAPSGTVRR1525 pKa = 11.84 FTRR1528 pKa = 11.84 AILVFSASLPAYY1540 pKa = 8.58 TPGIGISGKK1549 pKa = 10.57 GVLGLFNNNYY1559 pKa = 8.81 VLGMLSNAGAVVSGINGNADD1579 pKa = 3.56 GLRR1582 pKa = 11.84 LTGKK1586 pKa = 8.76 TISLDD1591 pKa = 3.25 GDD1593 pKa = 3.93 TVATGDD1599 pKa = 3.8 FWASQINAIKK1609 pKa = 10.31 INAANIVAGEE1619 pKa = 4.06 LDD1621 pKa = 3.47 ANIIHH1626 pKa = 6.62 VIHH1629 pKa = 7.08 LDD1631 pKa = 3.58 VSSLTGDD1638 pKa = 2.67 ITSFIKK1644 pKa = 10.98 SNWADD1649 pKa = 3.41 PYY1651 pKa = 11.39 GNNIDD1656 pKa = 3.99 IEE1658 pKa = 4.32 GSEE1661 pKa = 4.38 ISLHH1665 pKa = 6.96 DD1666 pKa = 3.54 NQKK1669 pKa = 10.58 NYY1671 pKa = 10.52 EE1672 pKa = 4.09 MLIKK1676 pKa = 10.63 ASEE1679 pKa = 3.74 IDD1681 pKa = 3.73 INNLNDD1687 pKa = 3.4 GSYY1690 pKa = 10.49 TKK1692 pKa = 10.26 ISNGNIEE1699 pKa = 4.55 MANSSPTGHH1708 pKa = 6.68 IEE1710 pKa = 4.27 SVGGLMLGEE1719 pKa = 4.07 NVVDD1723 pKa = 3.69 EE1724 pKa = 4.71 RR1725 pKa = 11.84 LNGIYY1730 pKa = 10.51 LIANTYY1736 pKa = 9.86 GKK1738 pKa = 10.84 GGTNHH1743 pKa = 6.71 NIDD1746 pKa = 3.89 SDD1748 pKa = 4.66 SYY1750 pKa = 9.81 WAADD1754 pKa = 3.73 DD1755 pKa = 4.41 VGIAYY1760 pKa = 10.47 RR1761 pKa = 11.84 NDD1763 pKa = 3.24 STNEE1767 pKa = 3.83 NFGVAYY1773 pKa = 9.76 RR1774 pKa = 11.84 YY1775 pKa = 10.19 NVTSGLNLFFAPVDD1789 pKa = 3.87 FNGYY1793 pKa = 9.31 KK1794 pKa = 10.51 FNIQGAGEE1802 pKa = 4.38 TFNLTWVSWSDD1813 pKa = 3.67 LSSGWKK1819 pKa = 9.64 YY1820 pKa = 10.1 PAIHH1824 pKa = 6.84 SYY1826 pKa = 11.08 GSAAKK1831 pKa = 10.33 GGIAIGGNAVYY1842 pKa = 10.7 AFGLAGRR1849 pKa = 11.84 TQLII1853 pKa = 3.9
Molecular weight: 196.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.389
IPC2_protein 4.495
IPC_protein 4.52
Toseland 4.317
ProMoST 4.673
Dawson 4.507
Bjellqvist 4.66
Wikipedia 4.444
Rodwell 4.355
Grimsley 4.228
Solomon 4.507
Lehninger 4.469
Nozaki 4.622
DTASelect 4.902
Thurlkill 4.355
EMBOSS 4.457
Sillero 4.647
Patrickios 3.223
IPC_peptide 4.507
IPC2_peptide 4.635
IPC2.peptide.svr19 4.589
Protein with the highest isoelectric point:
>tr|V9QKW1|V9QKW1_9CAUD Uncharacterized protein OS=Oenococcus phage phi9805 OX=1435411 PE=4 SV=1
MM1 pKa = 8.05 AIPKK5 pKa = 9.39 YY6 pKa = 9.93 IRR8 pKa = 11.84 KK9 pKa = 8.99 QSEE12 pKa = 4.06 YY13 pKa = 10.89 TPDD16 pKa = 3.54 FVNKK20 pKa = 7.64 SHH22 pKa = 7.55 RR23 pKa = 11.84 LLNYY27 pKa = 10.65 LSDD30 pKa = 4.17 NLNKK34 pKa = 10.31 NYY36 pKa = 10.04 RR37 pKa = 11.84 SSEE40 pKa = 3.97 LGFLFTVKK48 pKa = 9.9 DD49 pKa = 3.46 TGRR52 pKa = 11.84 MLRR55 pKa = 11.84 YY56 pKa = 9.17 YY57 pKa = 10.58 SEE59 pKa = 4.04 YY60 pKa = 10.77 LLRR63 pKa = 11.84 LGLPISTGDD72 pKa = 3.4 YY73 pKa = 10.62 GYY75 pKa = 11.46 KK76 pKa = 9.23 FTDD79 pKa = 3.49 KK80 pKa = 11.24 PEE82 pKa = 4.13 DD83 pKa = 3.55 LYY85 pKa = 11.49 RR86 pKa = 11.84 SAKK89 pKa = 9.33 RR90 pKa = 11.84 DD91 pKa = 3.38 QAIGQGAMEE100 pKa = 5.32 RR101 pKa = 11.84 YY102 pKa = 8.88 HH103 pKa = 7.42 LKK105 pKa = 10.23 IKK107 pKa = 10.36 AARR110 pKa = 11.84 KK111 pKa = 9.75 LEE113 pKa = 4.01 RR114 pKa = 11.84 QQKK117 pKa = 10.03 KK118 pKa = 10.08 LLAATKK124 pKa = 9.61 SEE126 pKa = 3.95 KK127 pKa = 10.62 HH128 pKa = 6.49 PIHH131 pKa = 6.8
Molecular weight: 15.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.162
IPC2_protein 9.458
IPC_protein 9.458
Toseland 9.94
ProMoST 9.677
Dawson 10.175
Bjellqvist 9.853
Wikipedia 10.365
Rodwell 10.555
Grimsley 10.262
Solomon 10.204
Lehninger 10.16
Nozaki 9.897
DTASelect 9.867
Thurlkill 10.014
EMBOSS 10.35
Sillero 10.087
Patrickios 9.78
IPC_peptide 10.189
IPC2_peptide 8.419
IPC2.peptide.svr19 8.457
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
13982
42
1853
254.2
28.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.066 ± 0.521
0.393 ± 0.083
6.601 ± 0.355
4.964 ± 0.483
4.391 ± 0.314
6.272 ± 0.605
1.445 ± 0.152
7.646 ± 0.427
8.096 ± 0.611
8.554 ± 0.44
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.181 ± 0.204
6.265 ± 0.334
2.768 ± 0.152
4.277 ± 0.28
3.326 ± 0.337
8.339 ± 0.585
6.387 ± 0.636
5.743 ± 0.256
1.287 ± 0.164
3.998 ± 0.329
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here