Gyrovirus GyV7-SF
Average proteome isoelectric point is 7.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A089RS37|A0A089RS37_9VIRU Dual specificity protein phosphatase VP2 OS=Gyrovirus GyV7-SF OX=1548711 PE=3 SV=1
MM1 pKa = 7.6 LSGGVEE7 pKa = 4.14 DD8 pKa = 4.5 TQPGCSIPAGGSEE21 pKa = 4.35 LPFRR25 pKa = 11.84 QEE27 pKa = 3.84 GQRR30 pKa = 11.84 GPSGAGGPGKK40 pKa = 8.25 TLNSHH45 pKa = 5.95 GTPIHH50 pKa = 6.6 EE51 pKa = 5.01 NGSTPQTPVTPSPRR65 pKa = 11.84 AHH67 pKa = 6.21 IRR69 pKa = 11.84 KK70 pKa = 7.87 TYY72 pKa = 10.3 GDD74 pKa = 3.33 VRR76 pKa = 11.84 ASNKK80 pKa = 9.32 FVSVGWDD87 pKa = 3.11 SLEE90 pKa = 5.15 RR91 pKa = 11.84 DD92 pKa = 3.95 PNWYY96 pKa = 9.31 RR97 pKa = 11.84 VNYY100 pKa = 9.84 NYY102 pKa = 10.44 TIATWLRR109 pKa = 11.84 NCSRR113 pKa = 11.84 THH115 pKa = 7.85 DD116 pKa = 4.5 EE117 pKa = 3.88 ICTCGSFRR125 pKa = 11.84 SHH127 pKa = 6.58 WFQEE131 pKa = 4.55 CSGLTDD137 pKa = 3.43 ACSQTEE143 pKa = 3.9 KK144 pKa = 11.21 DD145 pKa = 3.43 LGRR148 pKa = 11.84 LLSEE152 pKa = 4.75 GKK154 pKa = 9.64 RR155 pKa = 11.84 AKK157 pKa = 10.29 QKK159 pKa = 7.47 TTLPCNPTPHH169 pKa = 6.8 IRR171 pKa = 11.84 RR172 pKa = 11.84 KK173 pKa = 6.15 TVRR176 pKa = 11.84 WQDD179 pKa = 3.58 EE180 pKa = 4.17 QDD182 pKa = 4.1 DD183 pKa = 4.28 LADD186 pKa = 4.94 DD187 pKa = 4.27 STLSTEE193 pKa = 4.72 DD194 pKa = 3.34 DD195 pKa = 3.45 GGYY198 pKa = 8.77 TGDD201 pKa = 4.16 EE202 pKa = 4.49 DD203 pKa = 4.83 DD204 pKa = 4.82 FADD207 pKa = 3.87 IEE209 pKa = 4.46 PVNFDD214 pKa = 5.47 LDD216 pKa = 3.78 PQDD219 pKa = 4.42 AFIAALRR226 pKa = 11.84 GDD228 pKa = 4.1 SSTQGPAPTWW238 pKa = 3.31
Molecular weight: 26.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.847
IPC2_protein 4.876
IPC_protein 4.825
Toseland 4.711
ProMoST 4.978
Dawson 4.825
Bjellqvist 4.952
Wikipedia 4.724
Rodwell 4.711
Grimsley 4.622
Solomon 4.825
Lehninger 4.774
Nozaki 4.94
DTASelect 5.156
Thurlkill 4.724
EMBOSS 4.762
Sillero 4.991
Patrickios 3.948
IPC_peptide 4.825
IPC2_peptide 4.978
IPC2.peptide.svr19 4.962
Protein with the highest isoelectric point:
>tr|A0A089QEX9|A0A089QEX9_9VIRU CA1 OS=Gyrovirus GyV7-SF OX=1548711 PE=3 SV=1
MM1 pKa = 7.26 AHH3 pKa = 7.48 LYY5 pKa = 8.78 TRR7 pKa = 11.84 TVLRR11 pKa = 11.84 HH12 pKa = 5.82 KK13 pKa = 10.34 LLSRR17 pKa = 11.84 QAPEE21 pKa = 4.09 HH22 pKa = 5.51 TSGRR26 pKa = 11.84 PMEE29 pKa = 4.37 TSEE32 pKa = 4.47 QVTNSSALGGTVSKK46 pKa = 10.5 EE47 pKa = 3.4 ILIGIGSITITLSLPGSATVRR68 pKa = 11.84 VLTTRR73 pKa = 11.84 SAPVDD78 pKa = 3.69 LSEE81 pKa = 4.67 ATGSKK86 pKa = 10.23 NALDD90 pKa = 4.29 LRR92 pKa = 11.84 MPAAKK97 pKa = 9.7 RR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 ILVDD104 pKa = 3.26 CSVKK108 pKa = 10.62 EE109 pKa = 3.95 NEE111 pKa = 4.16 QNRR114 pKa = 11.84 KK115 pKa = 8.91 LRR117 pKa = 11.84 YY118 pKa = 9.07 LATQLLIYY126 pKa = 10.21 AARR129 pKa = 11.84 LL130 pKa = 3.41
Molecular weight: 14.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.648
IPC_protein 10.423
Toseland 10.657
ProMoST 10.335
Dawson 10.76
Bjellqvist 10.452
Wikipedia 10.965
Rodwell 10.965
Grimsley 10.804
Solomon 10.862
Lehninger 10.833
Nozaki 10.628
DTASelect 10.452
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 10.716
IPC_peptide 10.877
IPC2_peptide 9.37
IPC2.peptide.svr19 8.642
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
833
130
465
277.7
31.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.083 ± 0.707
1.681 ± 0.479
5.162 ± 1.547
3.962 ± 0.862
3.601 ± 1.168
6.963 ± 1.177
2.281 ± 0.102
3.481 ± 0.53
4.082 ± 0.274
7.443 ± 1.695
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.681 ± 0.476
4.202 ± 0.422
7.683 ± 1.123
3.842 ± 0.309
9.124 ± 0.915
8.403 ± 0.459
7.683 ± 1.089
5.042 ± 0.635
2.761 ± 0.837
3.842 ± 0.974
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here