Amino acid dipepetide frequency for Cyanophage MED4-117

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.975AlaAla: 8.975 ± 1.676
0.412AlaCys: 0.412 ± 0.172
4.611AlaAsp: 4.611 ± 0.664
4.364AlaGlu: 4.364 ± 0.589
3.294AlaPhe: 3.294 ± 0.488
6.34AlaGly: 6.34 ± 0.765
0.988AlaHis: 0.988 ± 0.353
5.846AlaIle: 5.846 ± 0.528
6.011AlaLys: 6.011 ± 1.088
6.34AlaLeu: 6.34 ± 0.779
1.317AlaMet: 1.317 ± 0.307
5.105AlaAsn: 5.105 ± 0.753
2.882AlaPro: 2.882 ± 0.335
3.294AlaGln: 3.294 ± 0.443
3.623AlaArg: 3.623 ± 0.631
5.434AlaSer: 5.434 ± 0.689
6.093AlaThr: 6.093 ± 0.924
5.187AlaVal: 5.187 ± 0.828
0.906AlaTrp: 0.906 ± 0.266
1.811AlaTyr: 1.811 ± 0.348
0.0AlaXaa: 0.0 ± 0.0
Cys
0.494CysAla: 0.494 ± 0.24
0.0CysCys: 0.0 ± 0.0
0.659CysAsp: 0.659 ± 0.216
0.247CysGlu: 0.247 ± 0.126
0.329CysPhe: 0.329 ± 0.2
0.576CysGly: 0.576 ± 0.255
0.329CysHis: 0.329 ± 0.165
0.823CysIle: 0.823 ± 0.247
0.906CysLys: 0.906 ± 0.332
0.906CysLeu: 0.906 ± 0.281
0.0CysMet: 0.0 ± 0.0
0.823CysAsn: 0.823 ± 0.261
0.247CysPro: 0.247 ± 0.136
0.329CysGln: 0.329 ± 0.138
0.741CysArg: 0.741 ± 0.293
0.494CysSer: 0.494 ± 0.256
0.823CysThr: 0.823 ± 0.249
0.329CysVal: 0.329 ± 0.162
0.082CysTrp: 0.082 ± 0.087
0.412CysTyr: 0.412 ± 0.205
0.0CysXaa: 0.0 ± 0.0
Asp
4.282AspAla: 4.282 ± 0.53
0.741AspCys: 0.741 ± 0.298
3.541AspAsp: 3.541 ± 0.726
2.717AspGlu: 2.717 ± 0.475
4.199AspPhe: 4.199 ± 0.602
4.94AspGly: 4.94 ± 1.039
0.823AspHis: 0.823 ± 0.198
4.776AspIle: 4.776 ± 0.735
3.211AspLys: 3.211 ± 0.68
5.352AspLeu: 5.352 ± 0.777
1.07AspMet: 1.07 ± 0.271
3.376AspAsn: 3.376 ± 0.65
2.388AspPro: 2.388 ± 0.404
1.811AspGln: 1.811 ± 0.345
2.882AspArg: 2.882 ± 0.491
3.211AspSer: 3.211 ± 0.575
4.364AspThr: 4.364 ± 0.555
3.788AspVal: 3.788 ± 0.448
0.494AspTrp: 0.494 ± 0.248
1.647AspTyr: 1.647 ± 0.399
0.0AspXaa: 0.0 ± 0.0
Glu
6.011GluAla: 6.011 ± 0.9
0.576GluCys: 0.576 ± 0.245
3.623GluAsp: 3.623 ± 0.697
4.035GluGlu: 4.035 ± 0.755
2.552GluPhe: 2.552 ± 0.458
3.211GluGly: 3.211 ± 0.608
0.247GluHis: 0.247 ± 0.138
4.117GluIle: 4.117 ± 0.661
4.446GluLys: 4.446 ± 0.822
6.422GluLeu: 6.422 ± 0.632
1.235GluMet: 1.235 ± 0.291
2.717GluAsn: 2.717 ± 0.552
2.552GluPro: 2.552 ± 0.508
3.047GluGln: 3.047 ± 0.535
1.729GluArg: 1.729 ± 0.374
3.705GluSer: 3.705 ± 0.553
4.858GluThr: 4.858 ± 0.846
3.87GluVal: 3.87 ± 0.432
0.906GluTrp: 0.906 ± 0.26
2.388GluTyr: 2.388 ± 0.443
0.0GluXaa: 0.0 ± 0.0
Phe
1.976PheAla: 1.976 ± 0.376
0.165PheCys: 0.165 ± 0.109
3.294PheAsp: 3.294 ± 0.56
3.129PheGlu: 3.129 ± 0.494
1.729PhePhe: 1.729 ± 0.332
3.376PheGly: 3.376 ± 0.616
0.906PheHis: 0.906 ± 0.244
2.8PheIle: 2.8 ± 0.58
2.388PheLys: 2.388 ± 0.508
2.47PheLeu: 2.47 ± 0.315
0.906PheMet: 0.906 ± 0.275
1.976PheAsn: 1.976 ± 0.396
1.811PhePro: 1.811 ± 0.378
1.235PheGln: 1.235 ± 0.292
2.552PheArg: 2.552 ± 0.42
3.705PheSer: 3.705 ± 0.816
3.788PheThr: 3.788 ± 0.693
2.8PheVal: 2.8 ± 0.638
0.412PheTrp: 0.412 ± 0.17
0.823PheTyr: 0.823 ± 0.268
0.0PheXaa: 0.0 ± 0.0
Gly
5.764GlyAla: 5.764 ± 0.844
0.576GlyCys: 0.576 ± 0.23
3.87GlyAsp: 3.87 ± 0.794
3.87GlyGlu: 3.87 ± 0.551
2.964GlyPhe: 2.964 ± 0.61
5.846GlyGly: 5.846 ± 1.087
1.07GlyHis: 1.07 ± 0.34
4.035GlyIle: 4.035 ± 0.819
3.541GlyLys: 3.541 ± 0.682
5.434GlyLeu: 5.434 ± 0.567
1.235GlyMet: 1.235 ± 0.495
3.623GlyAsn: 3.623 ± 0.52
0.741GlyPro: 0.741 ± 0.249
2.552GlyGln: 2.552 ± 0.44
2.223GlyArg: 2.223 ± 0.375
6.093GlySer: 6.093 ± 0.768
6.175GlyThr: 6.175 ± 1.118
4.364GlyVal: 4.364 ± 0.578
0.741GlyTrp: 0.741 ± 0.228
1.811GlyTyr: 1.811 ± 0.352
0.0GlyXaa: 0.0 ± 0.0
His
0.412HisAla: 0.412 ± 0.175
0.082HisCys: 0.082 ± 0.073
0.659HisAsp: 0.659 ± 0.286
0.741HisGlu: 0.741 ± 0.242
0.494HisPhe: 0.494 ± 0.166
0.988HisGly: 0.988 ± 0.318
0.082HisHis: 0.082 ± 0.082
0.576HisIle: 0.576 ± 0.226
1.07HisLys: 1.07 ± 0.317
1.564HisLeu: 1.564 ± 0.328
0.494HisMet: 0.494 ± 0.21
1.153HisAsn: 1.153 ± 0.335
0.494HisPro: 0.494 ± 0.212
0.906HisGln: 0.906 ± 0.259
0.741HisArg: 0.741 ± 0.202
0.906HisSer: 0.906 ± 0.199
1.07HisThr: 1.07 ± 0.335
0.823HisVal: 0.823 ± 0.237
0.082HisTrp: 0.082 ± 0.076
0.741HisTyr: 0.741 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
4.94IleAla: 4.94 ± 0.617
0.659IleCys: 0.659 ± 0.228
5.023IleAsp: 5.023 ± 0.595
4.529IleGlu: 4.529 ± 0.576
1.482IlePhe: 1.482 ± 0.306
3.87IleGly: 3.87 ± 0.953
1.07IleHis: 1.07 ± 0.247
3.047IleIle: 3.047 ± 0.457
5.434IleLys: 5.434 ± 0.748
4.117IleLeu: 4.117 ± 0.643
1.153IleMet: 1.153 ± 0.276
3.541IleAsn: 3.541 ± 0.532
3.376IlePro: 3.376 ± 0.421
2.388IleGln: 2.388 ± 0.415
2.388IleArg: 2.388 ± 0.492
4.364IleSer: 4.364 ± 0.712
7.081IleThr: 7.081 ± 0.916
3.705IleVal: 3.705 ± 0.548
0.247IleTrp: 0.247 ± 0.143
1.894IleTyr: 1.894 ± 0.383
0.0IleXaa: 0.0 ± 0.0
Lys
6.505LysAla: 6.505 ± 1.032
0.329LysCys: 0.329 ± 0.188
4.282LysAsp: 4.282 ± 0.57
4.776LysGlu: 4.776 ± 0.863
3.129LysPhe: 3.129 ± 0.559
3.541LysGly: 3.541 ± 0.815
0.741LysHis: 0.741 ± 0.241
5.187LysIle: 5.187 ± 0.527
8.152LysLys: 8.152 ± 1.416
6.011LysLeu: 6.011 ± 0.84
1.894LysMet: 1.894 ± 0.452
3.705LysAsn: 3.705 ± 0.627
1.647LysPro: 1.647 ± 0.42
2.882LysGln: 2.882 ± 0.543
4.035LysArg: 4.035 ± 0.805
2.882LysSer: 2.882 ± 0.519
4.693LysThr: 4.693 ± 0.531
4.693LysVal: 4.693 ± 0.761
0.329LysTrp: 0.329 ± 0.161
2.717LysTyr: 2.717 ± 0.563
0.0LysXaa: 0.0 ± 0.0
Leu
6.834LeuAla: 6.834 ± 0.954
1.153LeuCys: 1.153 ± 0.328
4.94LeuAsp: 4.94 ± 0.778
5.846LeuGlu: 5.846 ± 0.866
3.294LeuPhe: 3.294 ± 0.512
5.187LeuGly: 5.187 ± 0.648
0.988LeuHis: 0.988 ± 0.334
5.517LeuIle: 5.517 ± 0.498
5.599LeuLys: 5.599 ± 0.714
4.94LeuLeu: 4.94 ± 0.52
1.4LeuMet: 1.4 ± 0.339
4.446LeuAsn: 4.446 ± 0.619
4.035LeuPro: 4.035 ± 0.597
3.541LeuGln: 3.541 ± 0.543
2.882LeuArg: 2.882 ± 0.429
6.093LeuSer: 6.093 ± 0.76
6.422LeuThr: 6.422 ± 0.738
4.199LeuVal: 4.199 ± 0.6
0.659LeuTrp: 0.659 ± 0.2
2.141LeuTyr: 2.141 ± 0.406
0.0LeuXaa: 0.0 ± 0.0
Met
2.388MetAla: 2.388 ± 0.498
0.247MetCys: 0.247 ± 0.132
1.07MetAsp: 1.07 ± 0.229
1.729MetGlu: 1.729 ± 0.4
0.494MetPhe: 0.494 ± 0.173
0.659MetGly: 0.659 ± 0.241
0.412MetHis: 0.412 ± 0.174
0.906MetIle: 0.906 ± 0.279
1.894MetLys: 1.894 ± 0.44
1.4MetLeu: 1.4 ± 0.412
0.494MetMet: 0.494 ± 0.179
0.823MetAsn: 0.823 ± 0.255
0.494MetPro: 0.494 ± 0.169
0.741MetGln: 0.741 ± 0.256
0.823MetArg: 0.823 ± 0.267
1.647MetSer: 1.647 ± 0.277
1.235MetThr: 1.235 ± 0.308
0.494MetVal: 0.494 ± 0.188
0.576MetTrp: 0.576 ± 0.217
0.576MetTyr: 0.576 ± 0.18
0.0MetXaa: 0.0 ± 0.0
Asn
4.94AsnAla: 4.94 ± 0.594
1.564AsnCys: 1.564 ± 0.354
2.8AsnAsp: 2.8 ± 0.402
2.305AsnGlu: 2.305 ± 0.367
1.482AsnPhe: 1.482 ± 0.326
4.035AsnGly: 4.035 ± 0.585
0.741AsnHis: 0.741 ± 0.305
4.035AsnIle: 4.035 ± 0.532
3.211AsnLys: 3.211 ± 0.692
4.693AsnLeu: 4.693 ± 0.641
0.988AsnMet: 0.988 ± 0.32
3.541AsnAsn: 3.541 ± 0.634
2.305AsnPro: 2.305 ± 0.513
2.717AsnGln: 2.717 ± 0.433
2.305AsnArg: 2.305 ± 0.429
3.788AsnSer: 3.788 ± 0.621
3.294AsnThr: 3.294 ± 0.474
4.035AsnVal: 4.035 ± 0.565
0.329AsnTrp: 0.329 ± 0.154
1.564AsnTyr: 1.564 ± 0.401
0.0AsnXaa: 0.0 ± 0.0
Pro
2.635ProAla: 2.635 ± 0.311
0.247ProCys: 0.247 ± 0.195
2.964ProAsp: 2.964 ± 0.666
3.87ProGlu: 3.87 ± 0.697
1.4ProPhe: 1.4 ± 0.309
0.0ProGly: 0.0 ± 0.0
0.576ProHis: 0.576 ± 0.189
1.564ProIle: 1.564 ± 0.353
2.964ProLys: 2.964 ± 0.527
2.058ProLeu: 2.058 ± 0.417
0.741ProMet: 0.741 ± 0.233
2.141ProAsn: 2.141 ± 0.335
1.811ProPro: 1.811 ± 0.399
1.564ProGln: 1.564 ± 0.408
1.564ProArg: 1.564 ± 0.303
3.211ProSer: 3.211 ± 0.621
3.294ProThr: 3.294 ± 0.513
2.717ProVal: 2.717 ± 0.481
0.0ProTrp: 0.0 ± 0.0
1.317ProTyr: 1.317 ± 0.301
0.0ProXaa: 0.0 ± 0.0
Gln
3.458GlnAla: 3.458 ± 0.551
0.412GlnCys: 0.412 ± 0.208
1.647GlnAsp: 1.647 ± 0.42
1.729GlnGlu: 1.729 ± 0.429
2.058GlnPhe: 2.058 ± 0.426
2.717GlnGly: 2.717 ± 0.507
0.659GlnHis: 0.659 ± 0.239
3.458GlnIle: 3.458 ± 0.592
3.211GlnLys: 3.211 ± 0.644
3.788GlnLeu: 3.788 ± 0.586
1.153GlnMet: 1.153 ± 0.33
2.717GlnAsn: 2.717 ± 0.447
1.235GlnPro: 1.235 ± 0.255
1.729GlnGln: 1.729 ± 0.347
2.388GlnArg: 2.388 ± 0.37
2.47GlnSer: 2.47 ± 0.407
2.882GlnThr: 2.882 ± 0.381
2.141GlnVal: 2.141 ± 0.45
0.165GlnTrp: 0.165 ± 0.117
1.317GlnTyr: 1.317 ± 0.35
0.0GlnXaa: 0.0 ± 0.0
Arg
3.211ArgAla: 3.211 ± 0.596
0.247ArgCys: 0.247 ± 0.118
2.388ArgAsp: 2.388 ± 0.364
2.717ArgGlu: 2.717 ± 0.603
2.058ArgPhe: 2.058 ± 0.496
2.141ArgGly: 2.141 ± 0.435
0.494ArgHis: 0.494 ± 0.189
2.882ArgIle: 2.882 ± 0.477
3.788ArgLys: 3.788 ± 0.678
3.541ArgLeu: 3.541 ± 0.524
0.988ArgMet: 0.988 ± 0.319
2.223ArgAsn: 2.223 ± 0.452
1.4ArgPro: 1.4 ± 0.296
2.552ArgGln: 2.552 ± 0.512
1.976ArgArg: 1.976 ± 0.42
3.047ArgSer: 3.047 ± 0.6
2.223ArgThr: 2.223 ± 0.404
3.211ArgVal: 3.211 ± 0.428
0.741ArgTrp: 0.741 ± 0.273
1.317ArgTyr: 1.317 ± 0.435
0.0ArgXaa: 0.0 ± 0.0
Ser
6.834SerAla: 6.834 ± 0.918
0.659SerCys: 0.659 ± 0.176
3.705SerAsp: 3.705 ± 0.392
3.376SerGlu: 3.376 ± 0.594
3.705SerPhe: 3.705 ± 0.534
7.575SerGly: 7.575 ± 0.938
0.906SerHis: 0.906 ± 0.256
3.376SerIle: 3.376 ± 0.452
4.611SerLys: 4.611 ± 0.678
5.352SerLeu: 5.352 ± 0.57
1.482SerMet: 1.482 ± 0.279
3.458SerAsn: 3.458 ± 0.394
2.058SerPro: 2.058 ± 0.453
2.47SerGln: 2.47 ± 0.408
2.882SerArg: 2.882 ± 0.414
4.776SerSer: 4.776 ± 0.788
4.94SerThr: 4.94 ± 0.872
4.199SerVal: 4.199 ± 0.571
1.07SerTrp: 1.07 ± 0.336
1.729SerTyr: 1.729 ± 0.428
0.0SerXaa: 0.0 ± 0.0
Thr
6.011ThrAla: 6.011 ± 0.764
0.165ThrCys: 0.165 ± 0.104
3.952ThrAsp: 3.952 ± 0.505
5.023ThrGlu: 5.023 ± 0.919
3.541ThrPhe: 3.541 ± 0.402
5.928ThrGly: 5.928 ± 0.823
0.823ThrHis: 0.823 ± 0.294
4.858ThrIle: 4.858 ± 0.687
5.27ThrLys: 5.27 ± 0.615
7.41ThrLeu: 7.41 ± 0.998
1.07ThrMet: 1.07 ± 0.281
3.87ThrAsn: 3.87 ± 0.504
3.541ThrPro: 3.541 ± 0.584
3.129ThrGln: 3.129 ± 0.523
2.552ThrArg: 2.552 ± 0.493
5.681ThrSer: 5.681 ± 1.076
7.987ThrThr: 7.987 ± 1.292
6.093ThrVal: 6.093 ± 1.026
0.494ThrTrp: 0.494 ± 0.204
2.058ThrTyr: 2.058 ± 0.434
0.0ThrXaa: 0.0 ± 0.0
Val
4.529ValAla: 4.529 ± 0.666
0.988ValCys: 0.988 ± 0.306
3.87ValAsp: 3.87 ± 0.586
4.035ValGlu: 4.035 ± 0.473
2.635ValPhe: 2.635 ± 0.426
3.211ValGly: 3.211 ± 0.422
1.235ValHis: 1.235 ± 0.405
4.776ValIle: 4.776 ± 0.761
3.952ValLys: 3.952 ± 0.617
5.023ValLeu: 5.023 ± 0.688
0.906ValMet: 0.906 ± 0.29
2.964ValAsn: 2.964 ± 0.56
1.894ValPro: 1.894 ± 0.46
2.882ValGln: 2.882 ± 0.45
2.47ValArg: 2.47 ± 0.534
4.858ValSer: 4.858 ± 0.596
5.681ValThr: 5.681 ± 1.024
4.858ValVal: 4.858 ± 0.486
0.741ValTrp: 0.741 ± 0.196
1.976ValTyr: 1.976 ± 0.374
0.0ValXaa: 0.0 ± 0.0
Trp
0.494TrpAla: 0.494 ± 0.193
0.0TrpCys: 0.0 ± 0.0
0.494TrpAsp: 0.494 ± 0.181
0.412TrpGlu: 0.412 ± 0.175
0.576TrpPhe: 0.576 ± 0.264
0.741TrpGly: 0.741 ± 0.258
0.329TrpHis: 0.329 ± 0.16
0.247TrpIle: 0.247 ± 0.146
0.741TrpLys: 0.741 ± 0.23
0.906TrpLeu: 0.906 ± 0.29
0.247TrpMet: 0.247 ± 0.147
0.494TrpAsn: 0.494 ± 0.176
0.329TrpPro: 0.329 ± 0.157
0.329TrpGln: 0.329 ± 0.147
0.576TrpArg: 0.576 ± 0.221
1.153TrpSer: 1.153 ± 0.301
0.412TrpThr: 0.412 ± 0.19
0.741TrpVal: 0.741 ± 0.192
0.165TrpTrp: 0.165 ± 0.102
0.082TrpTyr: 0.082 ± 0.075
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.141TyrAla: 2.141 ± 0.386
0.329TyrCys: 0.329 ± 0.161
2.223TyrAsp: 2.223 ± 0.408
2.8TyrGlu: 2.8 ± 0.511
0.823TyrPhe: 0.823 ± 0.292
1.647TyrGly: 1.647 ± 0.283
0.659TyrHis: 0.659 ± 0.23
1.564TyrIle: 1.564 ± 0.294
1.729TyrLys: 1.729 ± 0.383
2.388TyrLeu: 2.388 ± 0.486
0.247TyrMet: 0.247 ± 0.133
1.976TyrAsn: 1.976 ± 0.399
1.4TyrPro: 1.4 ± 0.383
1.235TyrGln: 1.235 ± 0.332
1.811TyrArg: 1.811 ± 0.371
1.647TyrSer: 1.647 ± 0.299
2.141TyrThr: 2.141 ± 0.458
1.235TyrVal: 1.235 ± 0.352
0.329TyrTrp: 0.329 ± 0.162
1.564TyrTyr: 1.564 ± 0.451
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (12146 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski