Amino acid dipepetide frequency for Escherichia phage ST20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.589AlaAla: 10.589 ± 1.774
0.901AlaCys: 0.901 ± 0.289
6.083AlaAsp: 6.083 ± 0.721
7.885AlaGlu: 7.885 ± 0.994
3.98AlaPhe: 3.98 ± 0.502
6.609AlaGly: 6.609 ± 0.74
1.427AlaHis: 1.427 ± 0.293
5.632AlaIle: 5.632 ± 0.748
4.806AlaLys: 4.806 ± 0.745
8.261AlaLeu: 8.261 ± 0.834
2.403AlaMet: 2.403 ± 0.489
3.755AlaAsn: 3.755 ± 0.511
3.83AlaPro: 3.83 ± 0.481
3.154AlaGln: 3.154 ± 0.617
4.205AlaArg: 4.205 ± 0.539
5.332AlaSer: 5.332 ± 0.672
6.834AlaThr: 6.834 ± 0.856
7.134AlaVal: 7.134 ± 0.833
1.352AlaTrp: 1.352 ± 0.342
3.379AlaTyr: 3.379 ± 0.439
0.0AlaXaa: 0.0 ± 0.0
Cys
0.976CysAla: 0.976 ± 0.286
0.0CysCys: 0.0 ± 0.0
1.277CysAsp: 1.277 ± 0.396
0.976CysGlu: 0.976 ± 0.276
0.451CysPhe: 0.451 ± 0.185
0.826CysGly: 0.826 ± 0.277
0.3CysHis: 0.3 ± 0.144
0.751CysIle: 0.751 ± 0.259
0.451CysLys: 0.451 ± 0.155
0.601CysLeu: 0.601 ± 0.226
0.0CysMet: 0.0 ± 0.0
0.526CysAsn: 0.526 ± 0.206
0.3CysPro: 0.3 ± 0.15
0.375CysGln: 0.375 ± 0.166
0.901CysArg: 0.901 ± 0.294
0.3CysSer: 0.3 ± 0.179
0.676CysThr: 0.676 ± 0.264
0.526CysVal: 0.526 ± 0.226
0.225CysTrp: 0.225 ± 0.134
0.601CysTyr: 0.601 ± 0.262
0.0CysXaa: 0.0 ± 0.0
Asp
7.209AspAla: 7.209 ± 0.659
0.676AspCys: 0.676 ± 0.252
5.407AspAsp: 5.407 ± 0.618
4.881AspGlu: 4.881 ± 0.621
2.854AspPhe: 2.854 ± 0.356
6.533AspGly: 6.533 ± 0.939
0.676AspHis: 0.676 ± 0.214
3.98AspIle: 3.98 ± 0.477
2.854AspLys: 2.854 ± 0.441
4.581AspLeu: 4.581 ± 0.621
1.802AspMet: 1.802 ± 0.318
2.403AspAsn: 2.403 ± 0.493
1.802AspPro: 1.802 ± 0.344
0.751AspGln: 0.751 ± 0.227
2.704AspArg: 2.704 ± 0.554
2.854AspSer: 2.854 ± 0.526
3.53AspThr: 3.53 ± 0.498
4.281AspVal: 4.281 ± 0.533
1.352AspTrp: 1.352 ± 0.259
3.229AspTyr: 3.229 ± 0.57
0.0AspXaa: 0.0 ± 0.0
Glu
6.458GluAla: 6.458 ± 0.848
0.601GluCys: 0.601 ± 0.249
4.055GluAsp: 4.055 ± 0.651
5.182GluGlu: 5.182 ± 1.216
3.379GluPhe: 3.379 ± 0.498
5.407GluGly: 5.407 ± 0.791
1.051GluHis: 1.051 ± 0.303
3.53GluIle: 3.53 ± 0.536
4.806GluLys: 4.806 ± 0.764
6.083GluLeu: 6.083 ± 0.611
2.553GluMet: 2.553 ± 0.557
2.253GluAsn: 2.253 ± 0.429
1.727GluPro: 1.727 ± 0.498
3.605GluGln: 3.605 ± 0.78
3.379GluArg: 3.379 ± 0.661
2.854GluSer: 2.854 ± 0.507
3.304GluThr: 3.304 ± 0.461
5.257GluVal: 5.257 ± 0.711
1.502GluTrp: 1.502 ± 0.386
2.553GluTyr: 2.553 ± 0.394
0.0GluXaa: 0.0 ± 0.0
Phe
2.478PheAla: 2.478 ± 0.552
0.451PheCys: 0.451 ± 0.193
3.53PheAsp: 3.53 ± 0.591
2.704PheGlu: 2.704 ± 0.446
0.526PhePhe: 0.526 ± 0.219
2.628PheGly: 2.628 ± 0.472
0.375PheHis: 0.375 ± 0.184
2.854PheIle: 2.854 ± 0.461
2.478PheLys: 2.478 ± 0.444
2.178PheLeu: 2.178 ± 0.426
0.601PheMet: 0.601 ± 0.219
2.028PheAsn: 2.028 ± 0.388
1.277PhePro: 1.277 ± 0.305
1.277PheGln: 1.277 ± 0.254
1.953PheArg: 1.953 ± 0.302
2.628PheSer: 2.628 ± 0.583
2.403PheThr: 2.403 ± 0.313
2.478PheVal: 2.478 ± 0.465
0.601PheTrp: 0.601 ± 0.245
1.577PheTyr: 1.577 ± 0.367
0.0PheXaa: 0.0 ± 0.0
Gly
7.36GlyAla: 7.36 ± 0.816
1.051GlyCys: 1.051 ± 0.264
3.905GlyAsp: 3.905 ± 0.553
6.308GlyGlu: 6.308 ± 0.77
3.154GlyPhe: 3.154 ± 0.5
5.933GlyGly: 5.933 ± 0.684
1.427GlyHis: 1.427 ± 0.395
2.628GlyIle: 2.628 ± 0.416
4.806GlyLys: 4.806 ± 0.679
5.257GlyLeu: 5.257 ± 0.65
2.328GlyMet: 2.328 ± 0.555
3.605GlyAsn: 3.605 ± 0.657
2.178GlyPro: 2.178 ± 0.412
2.854GlyGln: 2.854 ± 0.465
3.53GlyArg: 3.53 ± 0.361
5.107GlySer: 5.107 ± 0.655
4.956GlyThr: 4.956 ± 0.872
5.107GlyVal: 5.107 ± 0.571
1.202GlyTrp: 1.202 ± 0.269
3.079GlyTyr: 3.079 ± 0.523
0.0GlyXaa: 0.0 ± 0.0
His
1.126HisAla: 1.126 ± 0.355
0.375HisCys: 0.375 ± 0.169
1.352HisAsp: 1.352 ± 0.32
0.751HisGlu: 0.751 ± 0.242
0.826HisPhe: 0.826 ± 0.262
1.126HisGly: 1.126 ± 0.327
0.676HisHis: 0.676 ± 0.307
0.601HisIle: 0.601 ± 0.191
1.126HisLys: 1.126 ± 0.3
1.202HisLeu: 1.202 ± 0.391
0.375HisMet: 0.375 ± 0.14
0.526HisAsn: 0.526 ± 0.207
0.901HisPro: 0.901 ± 0.304
0.676HisGln: 0.676 ± 0.215
0.901HisArg: 0.901 ± 0.287
0.601HisSer: 0.601 ± 0.23
0.751HisThr: 0.751 ± 0.301
1.352HisVal: 1.352 ± 0.371
0.075HisTrp: 0.075 ± 0.08
0.375HisTyr: 0.375 ± 0.167
0.0HisXaa: 0.0 ± 0.0
Ile
4.506IleAla: 4.506 ± 0.66
0.826IleCys: 0.826 ± 0.257
4.205IleAsp: 4.205 ± 0.519
3.454IleGlu: 3.454 ± 0.424
1.051IlePhe: 1.051 ± 0.309
3.304IleGly: 3.304 ± 0.486
0.526IleHis: 0.526 ± 0.22
2.929IleIle: 2.929 ± 0.414
3.68IleLys: 3.68 ± 0.536
3.68IleLeu: 3.68 ± 0.499
0.826IleMet: 0.826 ± 0.28
2.478IleAsn: 2.478 ± 0.378
3.079IlePro: 3.079 ± 0.409
1.877IleGln: 1.877 ± 0.336
2.553IleArg: 2.553 ± 0.418
3.98IleSer: 3.98 ± 0.731
5.332IleThr: 5.332 ± 0.764
3.53IleVal: 3.53 ± 0.492
1.051IleTrp: 1.051 ± 0.266
1.953IleTyr: 1.953 ± 0.422
0.0IleXaa: 0.0 ± 0.0
Lys
5.858LysAla: 5.858 ± 1.232
0.601LysCys: 0.601 ± 0.221
4.356LysAsp: 4.356 ± 0.5
3.98LysGlu: 3.98 ± 0.975
1.953LysPhe: 1.953 ± 0.335
3.68LysGly: 3.68 ± 0.501
1.202LysHis: 1.202 ± 0.258
1.877LysIle: 1.877 ± 0.455
3.605LysLys: 3.605 ± 0.634
4.956LysLeu: 4.956 ± 0.662
2.628LysMet: 2.628 ± 0.604
2.328LysAsn: 2.328 ± 0.401
2.628LysPro: 2.628 ± 0.564
2.028LysGln: 2.028 ± 0.554
3.755LysArg: 3.755 ± 0.683
3.004LysSer: 3.004 ± 0.544
3.68LysThr: 3.68 ± 0.503
3.454LysVal: 3.454 ± 0.65
0.451LysTrp: 0.451 ± 0.171
2.478LysTyr: 2.478 ± 0.364
0.0LysXaa: 0.0 ± 0.0
Leu
7.96LeuAla: 7.96 ± 0.957
0.826LeuCys: 0.826 ± 0.318
4.205LeuAsp: 4.205 ± 0.447
5.257LeuGlu: 5.257 ± 0.917
2.328LeuPhe: 2.328 ± 0.494
5.182LeuGly: 5.182 ± 0.543
1.352LeuHis: 1.352 ± 0.277
4.731LeuIle: 4.731 ± 0.596
4.581LeuLys: 4.581 ± 0.619
5.182LeuLeu: 5.182 ± 0.65
1.427LeuMet: 1.427 ± 0.31
3.68LeuAsn: 3.68 ± 0.603
3.83LeuPro: 3.83 ± 0.567
3.004LeuGln: 3.004 ± 0.527
4.281LeuArg: 4.281 ± 0.66
3.98LeuSer: 3.98 ± 0.529
5.858LeuThr: 5.858 ± 0.692
4.431LeuVal: 4.431 ± 0.511
0.901LeuTrp: 0.901 ± 0.287
2.103LeuTyr: 2.103 ± 0.422
0.0LeuXaa: 0.0 ± 0.0
Met
2.854MetAla: 2.854 ± 0.414
0.526MetCys: 0.526 ± 0.172
0.451MetAsp: 0.451 ± 0.244
1.352MetGlu: 1.352 ± 0.332
0.601MetPhe: 0.601 ± 0.217
1.953MetGly: 1.953 ± 0.49
0.375MetHis: 0.375 ± 0.162
1.502MetIle: 1.502 ± 0.337
1.652MetLys: 1.652 ± 0.387
1.727MetLeu: 1.727 ± 0.362
0.375MetMet: 0.375 ± 0.157
1.051MetAsn: 1.051 ± 0.32
1.502MetPro: 1.502 ± 0.401
0.901MetGln: 0.901 ± 0.273
1.577MetArg: 1.577 ± 0.298
2.028MetSer: 2.028 ± 0.341
1.877MetThr: 1.877 ± 0.311
1.577MetVal: 1.577 ± 0.35
0.15MetTrp: 0.15 ± 0.102
0.451MetTyr: 0.451 ± 0.228
0.0MetXaa: 0.0 ± 0.0
Asn
4.431AsnAla: 4.431 ± 0.403
0.601AsnCys: 0.601 ± 0.211
2.253AsnAsp: 2.253 ± 0.458
2.253AsnGlu: 2.253 ± 0.374
0.901AsnPhe: 0.901 ± 0.218
3.98AsnGly: 3.98 ± 0.558
0.375AsnHis: 0.375 ± 0.163
2.178AsnIle: 2.178 ± 0.451
1.953AsnLys: 1.953 ± 0.319
2.854AsnLeu: 2.854 ± 0.432
1.126AsnMet: 1.126 ± 0.319
2.103AsnAsn: 2.103 ± 0.437
2.403AsnPro: 2.403 ± 0.433
1.502AsnGln: 1.502 ± 0.348
1.877AsnArg: 1.877 ± 0.377
2.704AsnSer: 2.704 ± 0.404
2.854AsnThr: 2.854 ± 0.569
4.281AsnVal: 4.281 ± 0.451
0.601AsnTrp: 0.601 ± 0.226
1.427AsnTyr: 1.427 ± 0.38
0.0AsnXaa: 0.0 ± 0.0
Pro
3.68ProAla: 3.68 ± 0.533
0.375ProCys: 0.375 ± 0.161
3.004ProAsp: 3.004 ± 0.43
3.755ProGlu: 3.755 ± 0.643
2.028ProPhe: 2.028 ± 0.421
2.328ProGly: 2.328 ± 0.43
0.451ProHis: 0.451 ± 0.157
1.652ProIle: 1.652 ± 0.365
1.727ProLys: 1.727 ± 0.429
2.929ProLeu: 2.929 ± 0.463
0.826ProMet: 0.826 ± 0.259
1.727ProAsn: 1.727 ± 0.397
0.976ProPro: 0.976 ± 0.343
1.202ProGln: 1.202 ± 0.334
1.953ProArg: 1.953 ± 0.418
2.628ProSer: 2.628 ± 0.376
1.652ProThr: 1.652 ± 0.388
4.13ProVal: 4.13 ± 0.564
0.375ProTrp: 0.375 ± 0.162
1.126ProTyr: 1.126 ± 0.331
0.0ProXaa: 0.0 ± 0.0
Gln
3.454GlnAla: 3.454 ± 0.694
0.375GlnCys: 0.375 ± 0.17
2.028GlnAsp: 2.028 ± 0.392
2.253GlnGlu: 2.253 ± 0.451
1.277GlnPhe: 1.277 ± 0.298
1.652GlnGly: 1.652 ± 0.309
0.676GlnHis: 0.676 ± 0.235
2.178GlnIle: 2.178 ± 0.372
2.178GlnLys: 2.178 ± 0.464
3.53GlnLeu: 3.53 ± 0.483
0.976GlnMet: 0.976 ± 0.263
1.577GlnAsn: 1.577 ± 0.363
1.352GlnPro: 1.352 ± 0.322
2.253GlnGln: 2.253 ± 0.656
2.779GlnArg: 2.779 ± 0.365
2.178GlnSer: 2.178 ± 0.497
1.727GlnThr: 1.727 ± 0.467
2.178GlnVal: 2.178 ± 0.516
0.751GlnTrp: 0.751 ± 0.217
1.352GlnTyr: 1.352 ± 0.297
0.0GlnXaa: 0.0 ± 0.0
Arg
4.055ArgAla: 4.055 ± 0.588
0.676ArgCys: 0.676 ± 0.236
2.253ArgAsp: 2.253 ± 0.401
3.605ArgGlu: 3.605 ± 0.602
1.877ArgPhe: 1.877 ± 0.332
3.905ArgGly: 3.905 ± 0.457
1.051ArgHis: 1.051 ± 0.288
3.53ArgIle: 3.53 ± 0.451
4.581ArgLys: 4.581 ± 0.706
3.53ArgLeu: 3.53 ± 0.413
1.577ArgMet: 1.577 ± 0.329
2.929ArgAsn: 2.929 ± 0.381
1.502ArgPro: 1.502 ± 0.382
2.704ArgGln: 2.704 ± 0.445
4.055ArgArg: 4.055 ± 0.526
2.929ArgSer: 2.929 ± 0.382
2.929ArgThr: 2.929 ± 0.372
3.229ArgVal: 3.229 ± 0.379
0.601ArgTrp: 0.601 ± 0.253
1.577ArgTyr: 1.577 ± 0.327
0.0ArgXaa: 0.0 ± 0.0
Ser
5.032SerAla: 5.032 ± 0.565
0.601SerCys: 0.601 ± 0.15
3.53SerAsp: 3.53 ± 0.47
3.379SerGlu: 3.379 ± 0.503
2.628SerPhe: 2.628 ± 0.49
6.083SerGly: 6.083 ± 0.801
0.976SerHis: 0.976 ± 0.29
3.154SerIle: 3.154 ± 0.865
3.454SerLys: 3.454 ± 0.534
4.055SerLeu: 4.055 ± 0.603
1.126SerMet: 1.126 ± 0.282
2.478SerAsn: 2.478 ± 0.507
2.103SerPro: 2.103 ± 0.363
2.028SerGln: 2.028 ± 0.449
3.079SerArg: 3.079 ± 0.496
3.605SerSer: 3.605 ± 0.775
4.506SerThr: 4.506 ± 0.78
4.281SerVal: 4.281 ± 0.575
0.451SerTrp: 0.451 ± 0.153
1.953SerTyr: 1.953 ± 0.462
0.0SerXaa: 0.0 ± 0.0
Thr
7.96ThrAla: 7.96 ± 0.808
0.451ThrCys: 0.451 ± 0.177
4.055ThrAsp: 4.055 ± 0.436
3.755ThrGlu: 3.755 ± 0.57
3.004ThrPhe: 3.004 ± 0.583
6.233ThrGly: 6.233 ± 1.143
0.976ThrHis: 0.976 ± 0.351
4.205ThrIle: 4.205 ± 0.523
3.229ThrLys: 3.229 ± 0.425
5.407ThrLeu: 5.407 ± 0.672
0.901ThrMet: 0.901 ± 0.226
1.953ThrAsn: 1.953 ± 0.493
3.83ThrPro: 3.83 ± 0.598
1.577ThrGln: 1.577 ± 0.298
2.478ThrArg: 2.478 ± 0.316
3.454ThrSer: 3.454 ± 0.682
3.379ThrThr: 3.379 ± 0.668
4.431ThrVal: 4.431 ± 0.712
0.826ThrTrp: 0.826 ± 0.287
2.854ThrTyr: 2.854 ± 0.479
0.0ThrXaa: 0.0 ± 0.0
Val
6.759ValAla: 6.759 ± 0.549
0.451ValCys: 0.451 ± 0.183
4.281ValAsp: 4.281 ± 0.63
4.806ValGlu: 4.806 ± 0.669
1.802ValPhe: 1.802 ± 0.391
4.431ValGly: 4.431 ± 0.496
0.826ValHis: 0.826 ± 0.22
5.032ValIle: 5.032 ± 0.67
3.304ValLys: 3.304 ± 0.481
5.032ValLeu: 5.032 ± 0.577
1.427ValMet: 1.427 ± 0.363
3.229ValAsn: 3.229 ± 0.477
1.502ValPro: 1.502 ± 0.409
2.779ValGln: 2.779 ± 0.45
4.431ValArg: 4.431 ± 0.54
5.407ValSer: 5.407 ± 0.568
5.858ValThr: 5.858 ± 0.863
5.933ValVal: 5.933 ± 0.866
0.901ValTrp: 0.901 ± 0.289
2.779ValTyr: 2.779 ± 0.475
0.0ValXaa: 0.0 ± 0.0
Trp
1.277TrpAla: 1.277 ± 0.376
0.15TrpCys: 0.15 ± 0.108
1.051TrpAsp: 1.051 ± 0.238
0.451TrpGlu: 0.451 ± 0.201
0.976TrpPhe: 0.976 ± 0.324
0.826TrpGly: 0.826 ± 0.301
0.375TrpHis: 0.375 ± 0.207
0.225TrpIle: 0.225 ± 0.141
0.901TrpLys: 0.901 ± 0.241
1.502TrpLeu: 1.502 ± 0.27
0.526TrpMet: 0.526 ± 0.179
0.676TrpAsn: 0.676 ± 0.253
0.601TrpPro: 0.601 ± 0.257
0.676TrpGln: 0.676 ± 0.242
0.826TrpArg: 0.826 ± 0.276
0.601TrpSer: 0.601 ± 0.251
0.676TrpThr: 0.676 ± 0.212
1.202TrpVal: 1.202 ± 0.272
0.375TrpTrp: 0.375 ± 0.167
0.225TrpTyr: 0.225 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.53TyrAla: 3.53 ± 0.537
0.526TyrCys: 0.526 ± 0.204
3.079TyrAsp: 3.079 ± 0.799
2.553TyrGlu: 2.553 ± 0.429
1.427TyrPhe: 1.427 ± 0.406
3.154TyrGly: 3.154 ± 0.585
0.601TyrHis: 0.601 ± 0.206
1.652TyrIle: 1.652 ± 0.331
2.328TyrLys: 2.328 ± 0.365
2.553TyrLeu: 2.553 ± 0.395
0.601TyrMet: 0.601 ± 0.2
1.352TyrAsn: 1.352 ± 0.308
1.202TyrPro: 1.202 ± 0.353
1.502TyrGln: 1.502 ± 0.337
1.953TyrArg: 1.953 ± 0.467
2.328TyrSer: 2.328 ± 0.325
2.178TyrThr: 2.178 ± 0.451
2.178TyrVal: 2.178 ± 0.418
0.375TyrTrp: 0.375 ± 0.154
1.502TyrTyr: 1.502 ± 0.28
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (13317 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski