Amino acid dipepetide frequency for Rhodobacterales bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.158AlaAla: 14.158 ± 0.122	1.048AlaCys: 1.048 ± 0.026	6.734AlaAsp: 6.734 ± 0.068	7.421AlaGlu: 7.421 ± 0.077	4.432AlaPhe: 4.432 ± 0.056	9.997AlaGly: 9.997 ± 0.091	1.966AlaHis: 1.966 ± 0.036	6.055AlaIle: 6.055 ± 0.06	3.668AlaLys: 3.668 ± 0.055	11.983AlaLeu: 11.983 ± 0.096	3.204AlaMet: 3.204 ± 0.049	2.887AlaAsn: 2.887 ± 0.045	4.75AlaPro: 4.75 ± 0.065	3.338AlaGln: 3.338 ± 0.047	7.272AlaArg: 7.272 ± 0.072	6.483AlaSer: 6.483 ± 0.068	5.431AlaThr: 5.431 ± 0.071	8.161AlaVal: 8.161 ± 0.074	1.225AlaTrp: 1.225 ± 0.028	2.505AlaTyr: 2.505 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	0.963CysAla: 0.963 ± 0.025	0.129CysCys: 0.129 ± 0.008	0.562CysAsp: 0.562 ± 0.018	0.526CysGlu: 0.526 ± 0.018	0.356CysPhe: 0.356 ± 0.014	0.963CysGly: 0.963 ± 0.029	0.233CysHis: 0.233 ± 0.013	0.405CysIle: 0.405 ± 0.016	0.226CysLys: 0.226 ± 0.012	0.979CysLeu: 0.979 ± 0.024	0.17CysMet: 0.17 ± 0.01	0.235CysAsn: 0.235 ± 0.012	0.442CysPro: 0.442 ± 0.016	0.237CysGln: 0.237 ± 0.012	0.613CysArg: 0.613 ± 0.022	0.541CysSer: 0.541 ± 0.021	0.419CysThr: 0.419 ± 0.016	0.637CysVal: 0.637 ± 0.019	0.107CysTrp: 0.107 ± 0.009	0.2CysTyr: 0.2 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	6.468AspAla: 6.468 ± 0.078	0.537AspCys: 0.537 ± 0.017	3.43AspAsp: 3.43 ± 0.066	3.819AspGlu: 3.819 ± 0.047	2.415AspPhe: 2.415 ± 0.044	5.443AspGly: 5.443 ± 0.101	1.23AspHis: 1.23 ± 0.033	3.461AspIle: 3.461 ± 0.045	1.952AspLys: 1.952 ± 0.035	6.809AspLeu: 6.809 ± 0.071	1.563AspMet: 1.563 ± 0.034	1.515AspAsn: 1.515 ± 0.032	3.488AspPro: 3.488 ± 0.046	1.813AspGln: 1.813 ± 0.033	4.173AspArg: 4.173 ± 0.046	2.364AspSer: 2.364 ± 0.043	3.013AspThr: 3.013 ± 0.05	4.421AspVal: 4.421 ± 0.056	0.997AspTrp: 0.997 ± 0.025	1.635AspTyr: 1.635 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	7.533GluAla: 7.533 ± 0.076	0.382GluCys: 0.382 ± 0.019	3.645GluAsp: 3.645 ± 0.045	4.024GluGlu: 4.024 ± 0.063	1.985GluPhe: 1.985 ± 0.034	4.592GluGly: 4.592 ± 0.054	1.185GluHis: 1.185 ± 0.032	3.965GluIle: 3.965 ± 0.05	2.888GluLys: 2.888 ± 0.042	5.715GluLeu: 5.715 ± 0.066	1.677GluMet: 1.677 ± 0.034	2.136GluAsn: 2.136 ± 0.037	2.746GluPro: 2.746 ± 0.05	2.187GluGln: 2.187 ± 0.042	4.542GluArg: 4.542 ± 0.056	2.577GluSer: 2.577 ± 0.046	4.458GluThr: 4.458 ± 0.054	4.018GluVal: 4.018 ± 0.048	0.68GluTrp: 0.68 ± 0.02	1.033GluTyr: 1.033 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.375PheAla: 4.375 ± 0.055	0.451PheCys: 0.451 ± 0.017	2.763PheAsp: 2.763 ± 0.05	2.471PheGlu: 2.471 ± 0.037	1.578PhePhe: 1.578 ± 0.033	3.773PheGly: 3.773 ± 0.055	0.791PheHis: 0.791 ± 0.02	1.812PheIle: 1.812 ± 0.039	1.174PheLys: 1.174 ± 0.034	3.885PheLeu: 3.885 ± 0.06	0.899PheMet: 0.899 ± 0.024	1.125PheAsn: 1.125 ± 0.027	1.634PhePro: 1.634 ± 0.034	1.18PheGln: 1.18 ± 0.025	2.277PheArg: 2.277 ± 0.039	2.633PheSer: 2.633 ± 0.04	2.037PheThr: 2.037 ± 0.042	2.894PheVal: 2.894 ± 0.047	0.559PheTrp: 0.559 ± 0.018	0.958PheTyr: 0.958 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	8.582GlyAla: 8.582 ± 0.084	0.861GlyCys: 0.861 ± 0.025	4.639GlyAsp: 4.639 ± 0.09	5.062GlyGlu: 5.062 ± 0.068	3.709GlyPhe: 3.709 ± 0.05	7.082GlyGly: 7.082 ± 0.1	1.822GlyHis: 1.822 ± 0.038	4.702GlyIle: 4.702 ± 0.055	3.513GlyLys: 3.513 ± 0.056	8.947GlyLeu: 8.947 ± 0.086	2.255GlyMet: 2.255 ± 0.041	2.452GlyAsn: 2.452 ± 0.053	3.276GlyPro: 3.276 ± 0.045	2.722GlyGln: 2.722 ± 0.042	5.344GlyArg: 5.344 ± 0.067	5.058GlySer: 5.058 ± 0.068	4.906GlyThr: 4.906 ± 0.078	5.873GlyVal: 5.873 ± 0.071	1.282GlyTrp: 1.282 ± 0.029	2.442GlyTyr: 2.442 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	1.942HisAla: 1.942 ± 0.035	0.233HisCys: 0.233 ± 0.013	1.211HisAsp: 1.211 ± 0.027	1.116HisGlu: 1.116 ± 0.028	0.886HisPhe: 0.886 ± 0.022	1.798HisGly: 1.798 ± 0.035	0.541HisHis: 0.541 ± 0.02	0.921HisIle: 0.921 ± 0.024	0.578HisLys: 0.578 ± 0.021	2.098HisLeu: 2.098 ± 0.034	0.525HisMet: 0.525 ± 0.016	0.428HisAsn: 0.428 ± 0.017	1.248HisPro: 1.248 ± 0.026	0.581HisGln: 0.581 ± 0.015	1.254HisArg: 1.254 ± 0.029	0.983HisSer: 0.983 ± 0.023	0.842HisThr: 0.842 ± 0.025	1.489HisVal: 1.489 ± 0.033	0.284HisTrp: 0.284 ± 0.012	0.523HisTyr: 0.523 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	6.824IleAla: 6.824 ± 0.062	0.637IleCys: 0.637 ± 0.02	3.723IleAsp: 3.723 ± 0.051	3.52IleGlu: 3.52 ± 0.043	1.941IlePhe: 1.941 ± 0.041	5.036IleGly: 5.036 ± 0.063	0.936IleHis: 0.936 ± 0.02	2.31IleIle: 2.31 ± 0.045	1.515IleLys: 1.515 ± 0.033	5.086IleLeu: 5.086 ± 0.063	1.111IleMet: 1.111 ± 0.029	1.482IleAsn: 1.482 ± 0.027	2.362IlePro: 2.362 ± 0.038	1.346IleGln: 1.346 ± 0.03	3.317IleArg: 3.317 ± 0.041	3.592IleSer: 3.592 ± 0.053	2.779IleThr: 2.779 ± 0.04	4.249IleVal: 4.249 ± 0.063	0.652IleTrp: 0.652 ± 0.019	1.253IleTyr: 1.253 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	4.424LysAla: 4.424 ± 0.056	0.208LysCys: 0.208 ± 0.012	2.152LysAsp: 2.152 ± 0.043	1.963LysGlu: 1.963 ± 0.034	1.026LysPhe: 1.026 ± 0.025	2.873LysGly: 2.873 ± 0.047	0.635LysHis: 0.635 ± 0.023	1.984LysIle: 1.984 ± 0.037	1.5LysLys: 1.5 ± 0.039	3.501LysLeu: 3.501 ± 0.044	0.884LysMet: 0.884 ± 0.022	0.998LysAsn: 0.998 ± 0.024	1.952LysPro: 1.952 ± 0.042	1.081LysGln: 1.081 ± 0.031	2.43LysArg: 2.43 ± 0.041	2.291LysSer: 2.291 ± 0.036	2.31LysThr: 2.31 ± 0.041	2.767LysVal: 2.767 ± 0.054	0.38LysTrp: 0.38 ± 0.014	0.714LysTyr: 0.714 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	12.22LeuAla: 12.22 ± 0.1	0.981LeuCys: 0.981 ± 0.027	6.049LeuAsp: 6.049 ± 0.071	6.301LeuGlu: 6.301 ± 0.066	3.836LeuPhe: 3.836 ± 0.06	8.071LeuGly: 8.071 ± 0.086	1.76LeuHis: 1.76 ± 0.036	5.155LeuIle: 5.155 ± 0.07	4.291LeuLys: 4.291 ± 0.058	9.313LeuLeu: 9.313 ± 0.097	2.432LeuMet: 2.432 ± 0.044	2.935LeuAsn: 2.935 ± 0.039	5.35LeuPro: 5.35 ± 0.053	2.988LeuGln: 2.988 ± 0.046	6.079LeuArg: 6.079 ± 0.072	7.244LeuSer: 7.244 ± 0.067	5.859LeuThr: 5.859 ± 0.084	7.499LeuVal: 7.499 ± 0.079	1.074LeuTrp: 1.074 ± 0.028	2.131LeuTyr: 2.131 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	2.851MetAla: 2.851 ± 0.044	0.169MetCys: 0.169 ± 0.01	1.274MetAsp: 1.274 ± 0.029	1.347MetGlu: 1.347 ± 0.027	0.767MetPhe: 0.767 ± 0.021	1.753MetGly: 1.753 ± 0.032	0.431MetHis: 0.431 ± 0.016	1.518MetIle: 1.518 ± 0.031	1.112MetLys: 1.112 ± 0.024	2.397MetLeu: 2.397 ± 0.035	0.67MetMet: 0.67 ± 0.018	0.79MetAsn: 0.79 ± 0.02	1.443MetPro: 1.443 ± 0.03	0.793MetGln: 0.793 ± 0.021	1.725MetArg: 1.725 ± 0.037	1.908MetSer: 1.908 ± 0.037	1.972MetThr: 1.972 ± 0.03	1.729MetVal: 1.729 ± 0.033	0.199MetTrp: 0.199 ± 0.01	0.325MetTyr: 0.325 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	3.196AsnAla: 3.196 ± 0.05	0.254AsnCys: 0.254 ± 0.013	1.577AsnAsp: 1.577 ± 0.039	1.518AsnGlu: 1.518 ± 0.033	1.041AsnPhe: 1.041 ± 0.03	2.589AsnGly: 2.589 ± 0.052	0.525AsnHis: 0.525 ± 0.016	1.531AsnIle: 1.531 ± 0.032	0.849AsnLys: 0.849 ± 0.023	2.783AsnLeu: 2.783 ± 0.044	0.698AsnMet: 0.698 ± 0.02	0.773AsnAsn: 0.773 ± 0.02	1.84AsnPro: 1.84 ± 0.034	0.832AsnGln: 0.832 ± 0.024	1.888AsnArg: 1.888 ± 0.034	1.499AsnSer: 1.499 ± 0.033	1.369AsnThr: 1.369 ± 0.032	2.116AsnVal: 2.116 ± 0.039	0.461AsnTrp: 0.461 ± 0.017	0.71AsnTyr: 0.71 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	5.242ProAla: 5.242 ± 0.066	0.32ProCys: 0.32 ± 0.017	3.914ProAsp: 3.914 ± 0.053	3.957ProGlu: 3.957 ± 0.053	2.042ProPhe: 2.042 ± 0.032	4.212ProGly: 4.212 ± 0.057	0.987ProHis: 0.987 ± 0.027	2.21ProIle: 2.21 ± 0.038	1.737ProLys: 1.737 ± 0.034	4.427ProLeu: 4.427 ± 0.055	1.09ProMet: 1.09 ± 0.029	1.286ProAsn: 1.286 ± 0.029	2.057ProPro: 2.057 ± 0.044	1.587ProGln: 1.587 ± 0.032	2.423ProArg: 2.423 ± 0.043	2.773ProSer: 2.773 ± 0.044	2.281ProThr: 2.281 ± 0.033	4.247ProVal: 4.247 ± 0.052	0.575ProTrp: 0.575 ± 0.018	1.198ProTyr: 1.198 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.627GlnAla: 3.627 ± 0.051	0.188GlnCys: 0.188 ± 0.01	1.786GlnAsp: 1.786 ± 0.03	1.849GlnGlu: 1.849 ± 0.032	1.115GlnPhe: 1.115 ± 0.028	2.169GlnGly: 2.169 ± 0.034	0.584GlnHis: 0.584 ± 0.02	1.923GlnIle: 1.923 ± 0.034	1.284GlnLys: 1.284 ± 0.03	2.836GlnLeu: 2.836 ± 0.036	0.885GlnMet: 0.885 ± 0.022	0.935GlnAsn: 0.935 ± 0.023	1.533GlnPro: 1.533 ± 0.034	1.141GlnGln: 1.141 ± 0.034	2.07GlnArg: 2.07 ± 0.037	2.011GlnSer: 2.011 ± 0.035	1.936GlnThr: 1.936 ± 0.032	2.21GlnVal: 2.21 ± 0.036	0.32GlnTrp: 0.32 ± 0.014	0.581GlnTyr: 0.581 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	6.181ArgAla: 6.181 ± 0.069	0.491ArgCys: 0.491 ± 0.018	3.786ArgAsp: 3.786 ± 0.043	3.967ArgGlu: 3.967 ± 0.055	2.762ArgPhe: 2.762 ± 0.044	4.17ArgGly: 4.17 ± 0.047	1.504ArgHis: 1.504 ± 0.034	3.868ArgIle: 3.868 ± 0.05	2.843ArgLys: 2.843 ± 0.047	7.16ArgLeu: 7.16 ± 0.081	1.773ArgMet: 1.773 ± 0.033	1.91ArgAsn: 1.91 ± 0.033	3.051ArgPro: 3.051 ± 0.048	2.522ArgGln: 2.522 ± 0.045	4.691ArgArg: 4.691 ± 0.071	3.828ArgSer: 3.828 ± 0.055	3.296ArgThr: 3.296 ± 0.043	4.138ArgVal: 4.138 ± 0.052	0.789ArgTrp: 0.789 ± 0.024	1.546ArgTyr: 1.546 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	6.54SerAla: 6.54 ± 0.058	0.462SerCys: 0.462 ± 0.017	3.759SerAsp: 3.759 ± 0.053	3.636SerGlu: 3.636 ± 0.061	2.616SerPhe: 2.616 ± 0.042	6.29SerGly: 6.29 ± 0.082	1.16SerHis: 1.16 ± 0.025	3.002SerIle: 3.002 ± 0.044	1.851SerLys: 1.851 ± 0.035	5.957SerLeu: 5.957 ± 0.065	1.432SerMet: 1.432 ± 0.029	1.613SerAsn: 1.613 ± 0.032	2.784SerPro: 2.784 ± 0.043	1.826SerGln: 1.826 ± 0.034	3.838SerArg: 3.838 ± 0.049	3.657SerSer: 3.657 ± 0.054	2.854SerThr: 2.854 ± 0.049	4.494SerVal: 4.494 ± 0.054	0.799SerTrp: 0.799 ± 0.021	1.447SerTyr: 1.447 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	6.053ThrAla: 6.053 ± 0.063	0.447ThrCys: 0.447 ± 0.017	3.136ThrAsp: 3.136 ± 0.053	2.95ThrGlu: 2.95 ± 0.048	2.195ThrPhe: 2.195 ± 0.04	5.462ThrGly: 5.462 ± 0.077	1.049ThrHis: 1.049 ± 0.025	3.086ThrIle: 3.086 ± 0.058	1.489ThrLys: 1.489 ± 0.029	6.092ThrLeu: 6.092 ± 0.079	1.224ThrMet: 1.224 ± 0.025	1.416ThrAsn: 1.416 ± 0.034	3.066ThrPro: 3.066 ± 0.046	1.471ThrGln: 1.471 ± 0.031	3.23ThrArg: 3.23 ± 0.049	3.275ThrSer: 3.275 ± 0.046	2.791ThrThr: 2.791 ± 0.052	4.577ThrVal: 4.577 ± 0.059	0.689ThrTrp: 0.689 ± 0.022	1.32ThrTyr: 1.32 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	8.148ValAla: 8.148 ± 0.072	0.727ValCys: 0.727 ± 0.02	4.221ValAsp: 4.221 ± 0.053	4.595ValGlu: 4.595 ± 0.054	3.028ValPhe: 3.028 ± 0.047	5.327ValGly: 5.327 ± 0.062	1.373ValHis: 1.373 ± 0.026	4.119ValIle: 4.119 ± 0.052	2.376ValLys: 2.376 ± 0.041	7.621ValLeu: 7.621 ± 0.076	1.861ValMet: 1.861 ± 0.034	2.052ValAsn: 2.052 ± 0.036	3.75ValPro: 3.75 ± 0.049	2.002ValGln: 2.002 ± 0.037	4.503ValArg: 4.503 ± 0.061	5.102ValSer: 5.102 ± 0.062	4.588ValThr: 4.588 ± 0.076	5.633ValVal: 5.633 ± 0.068	0.925ValTrp: 0.925 ± 0.025	1.596ValTyr: 1.596 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.078TrpAla: 1.078 ± 0.026	0.147TrpCys: 0.147 ± 0.009	0.641TrpAsp: 0.641 ± 0.02	0.561TrpGlu: 0.561 ± 0.021	0.536TrpPhe: 0.536 ± 0.018	0.849TrpGly: 0.849 ± 0.025	0.29TrpHis: 0.29 ± 0.014	0.682TrpIle: 0.682 ± 0.018	0.483TrpLys: 0.483 ± 0.017	1.546TrpLeu: 1.546 ± 0.033	0.334TrpMet: 0.334 ± 0.014	0.441TrpAsn: 0.441 ± 0.018	0.652TrpPro: 0.652 ± 0.019	0.574TrpGln: 0.574 ± 0.016	0.92TrpArg: 0.92 ± 0.024	0.809TrpSer: 0.809 ± 0.024	0.732TrpThr: 0.732 ± 0.024	0.758TrpVal: 0.758 ± 0.022	0.198TrpTrp: 0.198 ± 0.011	0.312TrpTyr: 0.312 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.313TyrAla: 2.313 ± 0.042	0.271TyrCys: 0.271 ± 0.011	1.542TyrAsp: 1.542 ± 0.029	1.363TyrGlu: 1.363 ± 0.029	1.002TyrPhe: 1.002 ± 0.026	2.078TyrGly: 2.078 ± 0.035	0.481TyrHis: 0.481 ± 0.016	0.964TyrIle: 0.964 ± 0.023	0.746TyrLys: 0.746 ± 0.022	2.434TyrLeu: 2.434 ± 0.037	0.487TyrMet: 0.487 ± 0.018	0.641TyrAsn: 0.641 ± 0.019	1.138TyrPro: 1.138 ± 0.028	0.762TyrGln: 0.762 ± 0.022	1.687TyrArg: 1.687 ± 0.035	1.391TyrSer: 1.391 ± 0.031	1.143TyrThr: 1.143 ± 0.028	1.636TyrVal: 1.636 ± 0.032	0.351TyrTrp: 0.351 ± 0.015	0.635TyrTyr: 0.635 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5345 proteins (1748253 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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