Vibrio phage Valm-yong1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Yongunavirus; Vibrio virus Yong1

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3A3H8|A0A6M3A3H8_9CAUD Uncharacterized protein OS=Vibrio phage Valm-yong1 OX=2660715 PE=4 SV=1
MM1 pKa = 8.03SEE3 pKa = 3.58TTYY6 pKa = 11.51NKK8 pKa = 9.68TKK10 pKa = 10.71LEE12 pKa = 4.2HH13 pKa = 5.6LTDD16 pKa = 4.43YY17 pKa = 10.67IVSHH21 pKa = 6.63LNSNVLDD28 pKa = 3.92NKK30 pKa = 9.97IDD32 pKa = 3.28AWQEE36 pKa = 3.64NGSIVPNGEE45 pKa = 3.63DD46 pKa = 3.62RR47 pKa = 11.84GNGGYY52 pKa = 8.99IACYY56 pKa = 7.87WKK58 pKa = 10.69YY59 pKa = 9.89NAVVSIEE66 pKa = 4.2EE67 pKa = 4.22FPHH70 pKa = 6.69RR71 pKa = 11.84LLDD74 pKa = 3.91PRR76 pKa = 11.84CLLALVACWLSDD88 pKa = 3.28YY89 pKa = 9.43DD90 pKa = 3.73TTRR93 pKa = 11.84NEE95 pKa = 4.87DD96 pKa = 3.51EE97 pKa = 5.76LGDD100 pKa = 4.22PDD102 pKa = 5.43LSVDD106 pKa = 3.99VISSEE111 pKa = 4.24VADD114 pKa = 3.67VAIEE118 pKa = 4.1LEE120 pKa = 4.1MMEE123 pKa = 5.21PIEE126 pKa = 5.74LIPDD130 pKa = 4.04PAGMITWRR138 pKa = 11.84GEE140 pKa = 4.1TYY142 pKa = 10.22RR143 pKa = 11.84VQAVEE148 pKa = 4.07IYY150 pKa = 8.82TAEE153 pKa = 3.98EE154 pKa = 4.07AEE156 pKa = 4.56LVNEE160 pKa = 4.28ATNN163 pKa = 3.42

Molecular weight: 18.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3A3D7|A0A6M3A3D7_9CAUD Tail tube protein OS=Vibrio phage Valm-yong1 OX=2660715 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 9.83PQIKK6 pKa = 10.33VNEE9 pKa = 3.93RR10 pKa = 11.84DD11 pKa = 3.61VLNMQEE17 pKa = 4.23KK18 pKa = 10.29LAMLALPPKK27 pKa = 9.59KK28 pKa = 9.97RR29 pKa = 11.84VWILKK34 pKa = 8.76TLGRR38 pKa = 11.84WEE40 pKa = 4.03KK41 pKa = 10.88ANTRR45 pKa = 11.84KK46 pKa = 10.34RR47 pKa = 11.84IRR49 pKa = 11.84SQKK52 pKa = 10.17DD53 pKa = 2.97INGSALQPRR62 pKa = 11.84KK63 pKa = 9.72GKK65 pKa = 10.68KK66 pKa = 8.92KK67 pKa = 10.08GKK69 pKa = 7.82VLKK72 pKa = 10.7RR73 pKa = 11.84MAKK76 pKa = 10.02GLTPYY81 pKa = 9.92VRR83 pKa = 11.84NTNQLDD89 pKa = 4.07LTWSNKK95 pKa = 7.4LTAKK99 pKa = 10.03IAARR103 pKa = 11.84HH104 pKa = 5.53HH105 pKa = 6.33VGQKK109 pKa = 9.86QKK111 pKa = 7.51MTKK114 pKa = 10.08RR115 pKa = 11.84QMQKK119 pKa = 8.91RR120 pKa = 11.84WGKK123 pKa = 8.69PDD125 pKa = 3.3YY126 pKa = 10.6SAPCTKK132 pKa = 10.42GQARR136 pKa = 11.84KK137 pKa = 9.53LRR139 pKa = 11.84EE140 pKa = 3.72LGYY143 pKa = 8.73TVPRR147 pKa = 11.84KK148 pKa = 9.57SGKK151 pKa = 8.95GRR153 pKa = 11.84KK154 pKa = 8.94KK155 pKa = 9.72PSLRR159 pKa = 11.84EE160 pKa = 3.66LMATITHH167 pKa = 6.26GQAGQLIRR175 pKa = 11.84EE176 pKa = 5.01LSNQPNITSWDD187 pKa = 3.21IPLAEE192 pKa = 3.92RR193 pKa = 11.84QILGSKK199 pKa = 8.34EE200 pKa = 4.01RR201 pKa = 11.84EE202 pKa = 4.1VNRR205 pKa = 11.84QLIKK209 pKa = 10.45IFEE212 pKa = 4.04QAKK215 pKa = 8.65QRR217 pKa = 11.84KK218 pKa = 7.98

Molecular weight: 25.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

10085

37

910

210.1

23.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.26 ± 0.504

1.031 ± 0.108

5.711 ± 0.349

7.07 ± 0.439

4.135 ± 0.322

6.267 ± 0.283

1.874 ± 0.192

5.801 ± 0.249

6.515 ± 0.409

8.964 ± 0.397

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.846 ± 0.203

4.839 ± 0.209

3.877 ± 0.223

4.333 ± 0.32

4.74 ± 0.317

6.366 ± 0.321

5.959 ± 0.298

6.663 ± 0.321

1.715 ± 0.136

3.034 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski