Lederbergia galactosidilyticus
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4048 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q9XZZ1|A0A0Q9XZZ1_9BACI Uncharacterized protein OS=Lederbergia galactosidilyticus OX=217031 GN=ABB05_18285 PE=4 SV=1
MM1 pKa = 6.82 VQVKK5 pKa = 10.32 LFDD8 pKa = 4.9 CDD10 pKa = 3.85 HH11 pKa = 6.17 EE12 pKa = 4.44 QDD14 pKa = 4.98 LEE16 pKa = 4.3 EE17 pKa = 4.45 EE18 pKa = 4.22 MNEE21 pKa = 3.9 FLEE24 pKa = 4.61 QISPRR29 pKa = 11.84 DD30 pKa = 3.65 VVDD33 pKa = 3.52 IKK35 pKa = 11.66 YY36 pKa = 10.38 NVALIGDD43 pKa = 4.08 ASGEE47 pKa = 4.16 QVFCFSALIIYY58 pKa = 7.55 NTT60 pKa = 3.7
Molecular weight: 6.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.91
IPC_protein 3.795
Toseland 3.617
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.541
Solomon 3.757
Lehninger 3.706
Nozaki 3.91
DTASelect 4.062
Thurlkill 3.681
EMBOSS 3.719
Sillero 3.91
Patrickios 1.875
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.829
Protein with the highest isoelectric point:
>tr|A0A177ZGE5|A0A177ZGE5_9BACI Uncharacterized protein OS=Lederbergia galactosidilyticus OX=217031 GN=ABB05_22175 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPNKK9 pKa = 8.24 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 SKK14 pKa = 10.24 VHH16 pKa = 6.26 GFRR19 pKa = 11.84 QRR21 pKa = 11.84 MSTKK25 pKa = 10.0 NGRR28 pKa = 11.84 KK29 pKa = 8.83 ILAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.84 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.735
Grimsley 13.013
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.457
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4048
0
4048
1205544
26
1562
297.8
33.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.702 ± 0.035
0.684 ± 0.011
5.158 ± 0.031
7.639 ± 0.05
4.729 ± 0.036
6.717 ± 0.034
2.1 ± 0.018
8.195 ± 0.041
6.867 ± 0.033
9.894 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.79 ± 0.019
4.387 ± 0.025
3.674 ± 0.02
4.065 ± 0.024
3.919 ± 0.025
5.797 ± 0.028
5.328 ± 0.023
6.51 ± 0.029
1.188 ± 0.017
3.657 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here