Pan troglodytes polyomavirus 4
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7QKH3|K7QKH3_9POLY Minor capsid protein OS=Pan troglodytes polyomavirus 4 OX=1891736 PE=3 SV=1
MM1 pKa = 7.47 GGVLSIIVDD10 pKa = 4.37 IIEE13 pKa = 4.02 LTAEE17 pKa = 3.97 VTAATGFSLEE27 pKa = 4.31 AIINGEE33 pKa = 3.67 ALAAVEE39 pKa = 4.4 AQLSSLATVEE49 pKa = 4.17 GLSGVEE55 pKa = 4.03 ALASLGITTEE65 pKa = 4.02 QYY67 pKa = 11.46 SFLTSVPGMLSNAVGLGVVFQTVSGASALVSAGIATSYY105 pKa = 10.59 AKK107 pKa = 10.13 EE108 pKa = 3.83 VSVVNRR114 pKa = 11.84 NMALVPWRR122 pKa = 11.84 DD123 pKa = 2.93 PDD125 pKa = 3.7 YY126 pKa = 11.54 YY127 pKa = 11.05 DD128 pKa = 3.18 ILFPGVQSFSYY139 pKa = 10.2 AINVVADD146 pKa = 3.62 WSGSLFHH153 pKa = 7.57 AIGRR157 pKa = 11.84 YY158 pKa = 8.79 IWDD161 pKa = 3.83 TVTSEE166 pKa = 3.58 ARR168 pKa = 11.84 RR169 pKa = 11.84 QIGHH173 pKa = 7.72 ASNQVIVRR181 pKa = 11.84 GTHH184 pKa = 6.53 AFQDD188 pKa = 3.55 SLARR192 pKa = 11.84 ILEE195 pKa = 3.92 NARR198 pKa = 11.84 WVVQAGPTNIYY209 pKa = 10.79 NFLDD213 pKa = 3.23 NYY215 pKa = 10.15 YY216 pKa = 10.67 RR217 pKa = 11.84 EE218 pKa = 4.71 LPPVNPPQARR228 pKa = 11.84 QMYY231 pKa = 9.35 RR232 pKa = 11.84 RR233 pKa = 11.84 LGQKK237 pKa = 9.79 YY238 pKa = 7.99 RR239 pKa = 11.84 FARR242 pKa = 11.84 EE243 pKa = 3.82 AYY245 pKa = 9.82 QIPDD249 pKa = 3.79 RR250 pKa = 11.84 YY251 pKa = 10.88 NLANSEE257 pKa = 4.38 TEE259 pKa = 3.74 SGEE262 pKa = 3.98 TVEE265 pKa = 5.56 FYY267 pKa = 10.85 KK268 pKa = 10.99 HH269 pKa = 6.39 PGGANQRR276 pKa = 11.84 VTPDD280 pKa = 2.51 WMLPLILGLYY290 pKa = 10.53 GDD292 pKa = 4.64 ITPTWSTYY300 pKa = 6.34 VHH302 pKa = 6.85 EE303 pKa = 4.89 IEE305 pKa = 5.82 EE306 pKa = 4.25 EE307 pKa = 4.12 DD308 pKa = 3.49 EE309 pKa = 4.32 SQKK312 pKa = 10.88 KK313 pKa = 9.04 KK314 pKa = 9.72 RR315 pKa = 11.84 RR316 pKa = 11.84 RR317 pKa = 11.84 YY318 pKa = 9.21
Molecular weight: 35.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.999
IPC2_protein 5.118
IPC_protein 5.041
Toseland 4.978
ProMoST 5.156
Dawson 5.016
Bjellqvist 5.143
Wikipedia 4.863
Rodwell 4.94
Grimsley 4.902
Solomon 5.003
Lehninger 4.965
Nozaki 5.13
DTASelect 5.245
Thurlkill 4.965
EMBOSS 4.902
Sillero 5.207
Patrickios 3.745
IPC_peptide 5.016
IPC2_peptide 5.207
IPC2.peptide.svr19 5.174
Protein with the highest isoelectric point:
>tr|A0A5J6XVL7|A0A5J6XVL7_9POLY Middle T antigen OS=Pan troglodytes polyomavirus 4 OX=1891736 PE=4 SV=1
MM1 pKa = 7.72 GPRR4 pKa = 11.84 NLRR7 pKa = 11.84 SGGMHH12 pKa = 6.62 NFHH15 pKa = 7.16 PVHH18 pKa = 7.02 PSTSAPQLTTDD29 pKa = 3.64 EE30 pKa = 5.03 EE31 pKa = 4.33 EE32 pKa = 4.4 DD33 pKa = 3.15 PTYY36 pKa = 11.05 IVMKK40 pKa = 10.28 SLNPVMKK47 pKa = 9.97 KK48 pKa = 10.44 DD49 pKa = 3.67 PQPAPKK55 pKa = 9.45 PVSSDD60 pKa = 3.26 EE61 pKa = 4.2 EE62 pKa = 4.34 RR63 pKa = 11.84 PPTPPPRR70 pKa = 11.84 RR71 pKa = 11.84 IFSPLLPRR79 pKa = 11.84 PRR81 pKa = 11.84 RR82 pKa = 11.84 VPLLHH87 pKa = 6.4 MPEE90 pKa = 4.3 IPGGTLPARR99 pKa = 11.84 LTSPLTSLHH108 pKa = 6.49 RR109 pKa = 11.84 PPNVLPLNLLEE120 pKa = 6.15 KK121 pKa = 10.31 EE122 pKa = 4.08 IQTLRR127 pKa = 11.84 RR128 pKa = 11.84 QVSLAHH134 pKa = 7.16 RR135 pKa = 11.84 RR136 pKa = 11.84 RR137 pKa = 11.84 QRR139 pKa = 11.84 DD140 pKa = 3.25 LKK142 pKa = 10.88 FIMILSIFQILFLIILVMLYY162 pKa = 9.98 IVIKK166 pKa = 10.36 HH167 pKa = 5.54
Molecular weight: 19.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.706
IPC_protein 10.774
Toseland 11.023
ProMoST 11.023
Dawson 11.067
Bjellqvist 10.862
Wikipedia 11.359
Rodwell 11.155
Grimsley 11.111
Solomon 11.33
Lehninger 11.272
Nozaki 11.008
DTASelect 10.862
Thurlkill 11.008
EMBOSS 11.462
Sillero 11.023
Patrickios 10.906
IPC_peptide 11.345
IPC2_peptide 9.926
IPC2.peptide.svr19 8.842
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2108
167
730
351.3
40.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.602 ± 1.019
2.324 ± 0.608
5.455 ± 0.518
5.93 ± 0.359
4.317 ± 0.505
5.218 ± 0.77
2.277 ± 0.414
5.171 ± 0.384
6.072 ± 0.972
10.769 ± 0.904
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.226 ± 0.605
4.886 ± 0.473
7.258 ± 1.058
4.696 ± 0.274
5.977 ± 1.008
7.163 ± 0.671
4.934 ± 0.843
5.455 ± 0.85
1.091 ± 0.287
3.178 ± 0.409
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here