Micrococcus terreus
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2759 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I7ML73|A0A1I7ML73_9MICC Nucleoside-diphosphate-sugar epimerase OS=Micrococcus terreus OX=574650 GN=SAMN04487966_104247 PE=4 SV=1
MM1 pKa = 6.63 TTAHH5 pKa = 7.64 CIRR8 pKa = 11.84 HH9 pKa = 5.15 TMTALGAVTALILMTGCADD28 pKa = 3.27 QFRR31 pKa = 11.84 AIDD34 pKa = 3.72 SADD37 pKa = 3.52 SEE39 pKa = 4.92 GPSAHH44 pKa = 6.7 GAQSSTNEE52 pKa = 3.95 SRR54 pKa = 11.84 LEE56 pKa = 4.08 TIKK59 pKa = 10.33 TSGVLNACTTGDD71 pKa = 3.85 YY72 pKa = 11.12 PPFTEE77 pKa = 4.29 DD78 pKa = 3.11 TGDD81 pKa = 3.83 GEE83 pKa = 4.51 LTGIDD88 pKa = 3.21 VDD90 pKa = 4.1 MAADD94 pKa = 3.58 LAEE97 pKa = 4.11 TLEE100 pKa = 4.83 VDD102 pKa = 4.33 IEE104 pKa = 4.08 WHH106 pKa = 4.36 QTSWGDD112 pKa = 4.47 LMDD115 pKa = 4.67 TFLSDD120 pKa = 3.47 CDD122 pKa = 3.48 IAVGGISMNPPRR134 pKa = 11.84 AEE136 pKa = 3.51 QVFFSDD142 pKa = 6.18 PILEE146 pKa = 4.63 DD147 pKa = 3.27 GKK149 pKa = 9.63 TSIARR154 pKa = 11.84 CDD156 pKa = 3.41 EE157 pKa = 4.05 VQNYY161 pKa = 6.89 QTIEE165 pKa = 4.96 DD166 pKa = 3.8 INQPNVTSIFPEE178 pKa = 4.98 GGTNEE183 pKa = 3.89 AFARR187 pKa = 11.84 EE188 pKa = 4.23 HH189 pKa = 6.49 FPNGDD194 pKa = 3.68 LKK196 pKa = 10.18 THH198 pKa = 7.12 DD199 pKa = 4.13 NLTIFDD205 pKa = 4.08 EE206 pKa = 4.73 LVAGGADD213 pKa = 3.49 VMTTDD218 pKa = 3.77 RR219 pKa = 11.84 AEE221 pKa = 4.14 VLYY224 pKa = 10.24 IAHH227 pKa = 7.25 EE228 pKa = 4.02 YY229 pKa = 11.19 DD230 pKa = 3.58 EE231 pKa = 5.13 LCAVNPDD238 pKa = 3.22 EE239 pKa = 5.1 TYY241 pKa = 10.95 DD242 pKa = 3.93 FSVMGYY248 pKa = 7.76 MLPQGDD254 pKa = 4.43 VVFKK258 pKa = 10.6 EE259 pKa = 5.38 YY260 pKa = 10.92 VDD262 pKa = 3.4 QWLTIALKK270 pKa = 10.7 DD271 pKa = 3.53 GTYY274 pKa = 11.05 DD275 pKa = 3.89 EE276 pKa = 5.35 IAEE279 pKa = 4.11 PWVGNVEE286 pKa = 5.61 LIPEE290 pKa = 4.37 DD291 pKa = 3.36
Molecular weight: 31.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.783
IPC_protein 3.795
Toseland 3.579
ProMoST 3.923
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.617
Grimsley 3.49
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.113
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.91
Patrickios 1.265
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A1I7MSA4|A0A1I7MSA4_9MICC Adenylosuccinate synthetase OS=Micrococcus terreus OX=574650 GN=purA PE=3 SV=1
MM1 pKa = 7.64 PLKK4 pKa = 10.75 NLFRR8 pKa = 11.84 RR9 pKa = 11.84 AQSFARR15 pKa = 11.84 SPEE18 pKa = 3.84 GKK20 pKa = 10.33 RR21 pKa = 11.84 MINQAGRR28 pKa = 11.84 AARR31 pKa = 11.84 SRR33 pKa = 11.84 GGRR36 pKa = 11.84 RR37 pKa = 11.84 GGTRR41 pKa = 11.84 PAGGGLMGLAQQFLGRR57 pKa = 11.84 GRR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 3.22
Molecular weight: 6.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.464
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.076
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.14
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2759
0
2759
930413
41
2071
337.2
36.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.403 ± 0.053
0.568 ± 0.01
5.925 ± 0.043
6.22 ± 0.047
2.79 ± 0.03
9.045 ± 0.039
2.317 ± 0.02
3.821 ± 0.036
1.994 ± 0.032
10.095 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.075 ± 0.022
1.907 ± 0.023
5.744 ± 0.037
3.872 ± 0.028
7.158 ± 0.05
5.719 ± 0.032
6.344 ± 0.038
8.599 ± 0.039
1.533 ± 0.019
1.87 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here